Results 1 to 10 of about 751,337 (181)

Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling [PDF]

STAR Protocols, 2023
Summary: Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to obtain
Marc Emmenegger, Roland Worth, Sebastian Fiedler, Sean R.A. Devenish, Tuomas P.J. Knowles, Adriano Aguzzi   +5 more
doaj   +5 more sources

DG-Affinity: predicting antigen–antibody affinity with language models from sequences [PDF]

BMC Bioinformatics, 2023
Background Antibody-mediated immune responses play a crucial role in the immune defense of human body. The evolution of bioengineering has led the progress of antibody-derived drugs, showing promising efficacy in cancer and autoimmune disease therapy.
Ye Yuan, Qushuo Chen, Jun Mao, Guipeng Li, Xiaoyong Pan   +4 more
doaj   +2 more sources

Microfluidic antibody profiling after repeated SARS-CoV-2 vaccination links antibody affinity and concentration to impaired immunity and variant escape in patients on anti-CD20 therapy [PDF]

Frontiers in Immunology, 2023
BackgroundPatients with autoimmune/inflammatory conditions on anti-CD20 therapies, such as rituximab, have suboptimal humoral responses to vaccination and are vulnerable to poorer clinical outcomes following SARS-CoV-2 infection.
Ashley Priddey, Michael Xin Hua Chen-Xu, Michael Xin Hua Chen-Xu, Daniel James Cooper, Daniel James Cooper, Serena MacMillan, Georg Meisl, Catherine K. Xu, Myra Hosmillo, Ian G. Goodfellow, Rafael Kollyfas, Rainer Doffinger, John R. Bradley, John R. Bradley, Irina I. Mohorianu, Rachel Jones, Rachel Jones, Tuomas P. J. Knowles, Tuomas P. J. Knowles, Rona Smith, Rona Smith, Vasilis Kosmoliaptsis, Vasilis Kosmoliaptsis   +22 more
doaj   +2 more sources

Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sex [PDF]

EBioMedicine, 2021
Background: Limited knowledge exists regarding antibody affinity maturation following mRNA vaccination in naïve vs. COVID-19 recovered individuals and potential sex differences. Methods: We elucidated post-vaccination antibody profiles of 69 naïve and 17
Juanjie Tang, Gabrielle Grubbs, Youri Lee, Chang Huang, Supriya Ravichandran, David Forgacs, Hana Golding, Ted M Ross, Surender Khurana   +8 more
doaj   +2 more sources

Enhancing antibody affinity through experimental sampling of non-deleterious CDR mutations predicted by machine learning [PDF]

Communications Chemistry, 2023
The application of machine learning (ML) models to optimize antibody affinity to an antigen is gaining prominence. Unfortunately, the small and biased nature of the publicly available antibody-antigen interaction datasets makes it challenging to build an
Thomas Clark, Vidya Subramanian, Akila Jayaraman, Emmett Fitzpatrick, Ranjani Gopal, Niharika Pentakota, Troy Rurak, Shweta Anand, Alexander Viglione, Rahul Raman, Kannan Tharakaraman, Ram Sasisekharan   +11 more
doaj   +2 more sources

Optimizing Antibody Affinity and Developability Using a Framework–CDR Shuffling Approach—Application to an Anti-SARS-CoV-2 Antibody [PDF]

Viruses, 2022
The computational methods used for engineering antibodies for clinical development have undergone a transformation from three-dimensional structure-guided approaches to artificial-intelligence- and machine-learning-based approaches that leverage the ...
Ranjani Gopal, Emmett Fitzpatrick, Niharika Pentakota, Akila Jayaraman, Kannan Tharakaraman, Ishan Capila   +5 more
doaj   +2 more sources

Antibody affinity versus dengue morphology influences neutralization. [PDF]

PLoS Pathogens, 2021
Different strains within a dengue serotype (DENV1-4) can have smooth, or "bumpy" surface morphologies with different antigenic characteristics at average body temperature (37°C).
Guntur Fibriansah, Elisa X Y Lim, Jan K Marzinek, Thiam-Seng Ng, Joanne L Tan, Roland G Huber, Xin-Ni Lim, Valerie S Y Chew, Victor A Kostyuchenko, Jian Shi, Ganesh S Anand, Peter J Bond, James E Crowe, Shee-Mei Lok   +13 more
doaj   +2 more sources

Effect of trifluoroethanol on antibodies binding properties [PDF]

The Ukrainian Biochemical Journal, 2023
The studies on the influence of organic co-solvents on the structure and function of antibodies are of key interest, especially in view of antibodies broad use as recognizing elements in different analytical systems.
S. A. Bobrovnik, M. O. Demchenko, S. V. Komisarenko   +2 more
doaj   +1 more source

The prominent role of a CDR1 somatic hypermutation for convergent IGHV3-53/3-66 antibodies in binding to SARS-CoV-2

Emerging Microbes and Infections, 2022
In the fight against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), monoclonal antibodies (mAbs) serve as key strategies for the rapid prevention and treatment of COVID-19. However, analysis to fully characterize functional SARS-CoV-2 mAbs
Xiaolong Tian, Xiaoyi Zhu, Wenping Song, Zhenlin Yang, Yanling Wu, Tianlei Ying   +5 more
doaj   +1 more source

Simultaneous Maturation of Single Chain Antibody Stability and Affinity by CHO Cell Display

Bioengineering, 2022
Antibody stability and affinity are two important features of its applications in therapy and diagnosis. Antibody display technologies such as yeast and bacterial displays have been successfully used for improving both affinity and stability.
Ruiqi Luo, Baole Qu, Lili An, Yun Zhao, Yang Cao, Peng Ren, Haiying Hang   +6 more
doaj   +1 more source