Antibody Affinity Shapes the Choice between Memory and Germinal Center B Cell Fates. [PDF]
Cell, 2020 Viant C +7 more
europepmc +2 more sources
AID activity in B cells strongly correlates with polyclonal antibody affinity maturation in-vivo following pandemic 2009-H1N1 vaccination in humans. [PDF]
PLoS Pathogens, 2012 The role of Activation-Induced Cytidine Deaminase (AID) in somatic hypermutation and polyclonal antibody affinity maturation has not been shown for polyclonal responses in humans. We investigated whether AID induction in human B cells following H1N1pdm09
Surender Khurana +3 more
doaj +1 more source
Antibodies with infinite affinity [PDF]
Proceedings of the National Academy of Sciences, 2001 Here we report an approach to the design and production of antibody/ligand pairs, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate.
A J, Chmura, M S, Orton, C F, Meares
openaire +2 more sources
Non-Affinity Purification of Antibodies
Antibodies, 2023 Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review.
Tsutomu Arakawa +7 more
openaire +3 more sources
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. [PDF]
PLoS ONE, 2014 The optimization of antibodies is a desirable goal towards the development of better therapeutic strategies. The antibody 11K2 was previously developed as a therapeutic tool for inflammatory diseases, and displays very high affinity (4.6 pM) for its ...
Masato Kiyoshi +8 more
doaj +1 more source
Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal [PDF]
, 2016 A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein.
A Hayhurst +38 more
core +13 more sources
Alternative Hapten Design for Zearalenone Immunoreagent Generation
Toxins, 2022 Appropriate hapten design and synthesis have been identified as critical steps to generate high-performance immunoreagents and to develop sensitive and selective immunoanalytical methods.
Antonio Abad-Fuentes +5 more
doaj +1 more source
Effect of β-alanine on humoral immune response in low-dose allergy model
Медицинская иммунология, 2021 At the present time, the efforts of many research groups around the world are aimed at finding new factors triggering the allergic sensitization process linked with IgE synthesis to harmless allergens.
D. B. Chudakov +4 more
doaj +1 more source
Effect of trifluoroethanol on antibody reactivity against corresponding and nonrelated antigens [PDF]
The Ukrainian Biochemical Journal, 2018 The ability of antibodies to switch between specific and nonspecific recognition of antigens under various factors is the key issue. Here we demonstrate that 2,2,2-trifluoroethanol (TFE) is one of these factors influencing the ability of monoclonal ...
S. A. Bobrovnik +2 more
doaj +1 more source
Higher Affinity Antibodies Bind With Lower Hydration and Flexibility in Large Scale Simulations
Frontiers in Immunology, 2022 We have carried out a long-timescale simulation study on crystal structures of nine antibody-antigen pairs, in antigen-bound and antibody-only forms, using molecular dynamics with enhanced sampling and an explicit water model to explore interface ...
Mabel T. Y. Wong +5 more
doaj +1 more source