Sense and antisense RNA are not toxic in Drosophila models of C9orf72-associated ALS/FTD
Acta Neuropathologica, 2018 A GGGGCC hexanucleotide repeat expansion in the C9orf72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia.
T. Moens +11 more
semanticscholar +1 more source
Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay
BioTechniques, 1997 Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR ...
Mary Jo J. Davis +2 more
doaj +1 more source
Antisense RNA Design, Delivery, and Analysis [PDF]
, 2022 This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention.
core +2 more sources
The application of antisense technology for crop improvement: A review
Cogent Food & Agriculture, 2021 The global population is increasing alarmingly, which calls for efficient methods of food production. Since domestication, people have been working on improving food crops for production and productivity.
Tadesse Tilahun +3 more
doaj +1 more source
Cell Transplantation, 2012 Tissue factor (TF) expression on islets has been shown to trigger instant blood-mediated inflammatory reaction (IBMIR), leading to rapid islet loss in portal vein islet transplantation.
Xiaoqian Ma +8 more
doaj +1 more source
Retrovirology, 2006 Background While viruses have long been shown to capitalize on their limited genomic size by utilizing both strands of DNA or complementary DNA/RNA intermediates to code for viral proteins, it has been assumed that human retroviruses have all their major
Hsiao Chiu-Bin +6 more
doaj +1 more source
Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA
Nature Communications, 2023 The expanded hexanucleotide GGGGCC repeat mutation in the C9orf72 gene is the main genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia.
Mirjana Malnar Črnigoj +10 more
doaj +1 more source
Programmable control of bacterial gene expression with the combined CRISPR and antisense RNA system
Nucleic Acids Research, 2016 A central goal of synthetic biology is to implement diverse cellular functions by predictably controlling gene expression. Though research has focused more on protein regulators than RNA regulators, recent advances in our understanding of RNA folding and
Young-Je Lee +3 more
semanticscholar +1 more source
Meeting review : ESF workshop on "Impact of nucleic acid chemistry on gene function analysis: antisense, aptamers, ribozymes and RNAi" [PDF]
, 2008 The shortage of functional information compared to the abundance of sequence information characterizes today’s situation in functional genomics. For many years the knock-down of a gene’s product has been the most powerful way of analysing its function ...
Prydz, Hans +3 more
core +1 more source
An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]
, 2013 Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Darren M. Gowers (485354) +29 more
core +2 more sources