Incorporation of aptamers in the terminal loop of shRNAs yields an effective and novel combinatorial targeting strategy. [PDF]
, 2017 Gene therapy by engineering patient's own blood cells to confer HIV resistance can potentially lead to a functional cure for AIDS. Toward this goal, we have previously developed an anti-HIV lentivirus vector that deploys a combination of shRNA, ribozyme ...
Castanotto, Daniela +4 more
core +1 more source
DNA module platform for developing colorimetric aptamer sensors
BioTechniques, 2016 Here we present a DNA module platform for developing simple aptamer sensors based on a microarray format combined with secondary structure prediction in silico.
Yasuyuki Tomita +3 more
doaj +1 more source
Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity. [PDF]
, 2010 The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of ...
Carothers, James M +4 more
core +2 more sources
Aptamer-based multiplexed proteomic technology for biomarker discovery [PDF]
, 2010 Interrogation of the human proteome in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology. We present a new aptamer-based proteomic technology for biomarker discovery capable of simultaneously measuring thousands
A Keller +115 more
core +13 more sources
Enzyme-mimic activity study of superstable and ultrasmall graphene encapsuled CoRu nanocrystal
APL Materials, 2021 Nanozymes hold huge potential in various fields, but their much lower catalytic activities than natural enzymes greatly limit their extensive applications. Recent studies indicated that an enhanced enzyme-like activity could be obtained by decreasing the
Phouphien Keoingthong +7 more
doaj +1 more source
HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors [PDF]
, 2005 RNA and DNA aptamers specific for HIV-1 reverse transcriptase (RT) can inhibit reverse transcription in vitro. RNA aptamers have been shown to potently block HIV-1 replication in culture.
Fisher, Timothy S +2 more
core +3 more sources
Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays. [PDF]
, 2015 A strategy to extend the detection range of weakly-binding targets is reported that takes advantage of fluorescence resonance energy transfer (FRET)-based bioassays based on molecular beacon aptamers (MBAs) and cationic conjugated polyelectrolytes (CPEs).
Bazan, Guillermo C +5 more
core +1 more source
Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer
Molecular Therapy: Nucleic Acids, 2015 Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter
Felipe Opazo +6 more
doaj +1 more source
Advanced Science, 2022 Increasing antibiotic resistance becomes a serious threat to public health. Photothermal therapy (PTT) and antibacterial enzyme‐based therapy are promising nonresistant strategies for efficiently killing drug‐resistant bacteria.
Zhangkun Liu +6 more
doaj +1 more source
Design principles for riboswitch function [PDF]
, 2009 Scientific and technological advances that enable the tuning of integrated regulatory components to match network and system requirements are critical to reliably control the function of biological systems. RNA provides a promising building block for the
A Belle +62 more
core +4 more sources