Results 81 to 90 of about 68,358 (287)

Aptamers as Therapeutics [PDF]

Annual Review of Pharmacology and Toxicology, 2017
Aptamers are single-stranded nucleic acid molecules that bind to and inhibit proteins and are commonly produced by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers undergo extensive pharmacological revision, which alters affinity, specificity, and therapeutic half-life, tailoring each drug for a specific clinical need.
Shahid M, Nimjee, Rebekah R, White, Richard C, Becker, Bruce A, Sullenger   +3 more
openaire   +2 more sources

Unlocking the Potential of MXene‐Based Electrochemical Biosensors: A Review of Biofunctionalization Strategies and Biosensing Principles

Advanced Materials Technologies, EarlyView.
ABSTRACT Electrochemical biosensors enable the accurate and timely detection of clinical biomarkers, improving healthcare and precision medicine. MXene nanosheets, a class of 2D transition metal carbides, nitrides, and carbonitrides, are promising materials for developing next‐generation electrochemical biosensors due to their unique physicochemical ...
Muhsin Ali, Sharat Chandra Barman, Isobel Steer, Aya H. Alshareef, Dana Alsulaiman   +4 more
wiley   +1 more source

Phosphorylated lipid-conjugated oligonucleotide selectively anchors on cell membranes with high alkaline phosphatase expression

Nature Communications, 2019
Membrane-anchored DNA probes have been used to study molecular interactions and control cell assembly, but are not selective for different cell membranes. Here the authors develop a lipid-conjugated oligonucleotide for alkaline phosphatase-dependent cell
Cheng Jin, Jiaxuan He, Jianmei Zou, Wenjing Xuan, Ting Fu, Ruowen Wang, Weihong Tan   +6 more
doaj   +1 more source

Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer

Current Research in Structural Biology, 2020
The high-resolution crystal structure of HIV-1 reverse transcriptase (RT) bound to a 38-mer DNA hairpin aptamer with low pM affinity was previously described.
Steve Tuske, Jie Zheng, Erik D. Olson, Francesc X. Ruiz, Bruce D. Pascal, Anthony Hoang, Joseph D. Bauman, Kalyan Das, Jeffrey J. DeStefano, Karin Musier-Forsyth, Patrick R. Griffin, Eddy Arnold   +11 more
doaj   +1 more source

Methods and Applications of In Silico Aptamer Design and Modeling

International Journal of Molecular Sciences, 2020
Aptamers are nucleic acid analogues of antibodies with high affinity to different targets, such as cells, viruses, proteins, inorganic materials, and coenzymes. Empirical approaches allow the design of in vitro aptamers that bind particularly to a target
A. A. Buglak, A. Samokhvalov, A. Zherdev, B. Dzantiev   +3 more
semanticscholar   +1 more source

Preservation of Extracellular Vesicle Integrity and Cargo Using Spiral Microfluidic Chip‐Based Separation

Advanced Materials Technologies, EarlyView.
A spiral microfluidic chip is compared with ultrafiltration for the separation of extracellular vesicles (EVs). The method preserves miRNA cargo and membrane integrity more effectively, resulting in intact vesicle morphology and consistent surface biomarker expression.
Huiseop Lee, Sehyeon Kim, Dasom Kong, Hye‐min Park, Hyun‐Ji Park, Jinsik Kim   +5 more
wiley   +1 more source

Engineering Osteoimmune Responses with Functionalized Orthopedic Implants for Post‐Operative Osteosarcoma Treatment

Advanced Science, EarlyView.
Osteosarcoma is the most common primary bone tumor with limited treatment options and a terrible prognosis. This review provides a comprehensive summary of the recent development of osteoimmunomodulatory implants for post‐operative osteosarcoma treatment, of which the potential utility in evoking durable anti‐osteosarcoma immunity and accelerating bone
Yilong Dong, Lingshuang Wang, Hui Zhang, Menghuan Li, Zhong Luo, Yan Hu, Chunyuan Cai   +6 more
wiley   +1 more source

Recent Advances in Aptamer-Based Point-of-Care Testing

Sensors
Aptamers refer to a class of oligonucleotide probes that have demonstrated remarkable capabilities beyond mere genetic coding, including the unique ability to recognize and selectively bind to specific molecular targets.
Senlin Luo, Xiaotian Zhang, Yuzhuo Zhang, Xiaofeng Qu, Qiru Sun, Tianhuan Peng, Quan Yuan   +6 more
doaj   +1 more source

Aptamer-based lateral flow assay for point of care cortisol detection in sweat

Sensors and Actuators B: Chemical, 2019
A new aptamer-based lateral flow strip assay has been designed and developed for on-site rapid detection of cortisol in sweat. Cortisol in sweat has been identified as a key biomarker to monitor physiological stress.
Shima Dalirirad, A. Steckl
semanticscholar   +1 more source

APOE‐stratified Proteomic and Metabolomic Analysis Reveals Mitochondrial Dysfunction Inflammation and Lipid Dysregulation in Alzheimer's Disease

Advanced Science, EarlyView.
A large‐scale multiomic dataset (proteomic and metabolomic) comprising 3,060 plasma samples were analyzed to identify proteins, metabolites, pathways, and protein‐associated drugs linked to Alzheimer’s Disease (AD) independently of apolipoprotein E (APOE). AD was associated with a distinct molecular signature that captures.
Fuhai Li, Yike Chen, Daniel Western, Muhammad Ali, Menghan Liu, Katherine Gong, Ying Xu, Joseph Lowery, David M. Holtzman, Chloe Robins, John D. Eicher, Yen‐Ning Huang, ShiWei Liu, Tamina Park, Andrew J. Saykin, Kwangsik Nho, Mahdi Moqri, Richard C. Mohs, Amelia Farinas, Patricia Moran‐Losada, Hamilton Se‐Hwee Oh, Tony Wyss‐Coray, Carlos Cruchaga   +22 more
wiley   +1 more source