Results 11 to 20 of about 19,186 (244)

Aptamers and Cancer

Asian Pacific Journal of Cancer Biology, 2016
Oligonucleotides Aptamers are single strands of DNA and RNA with the length of 20-100 nucleotides or peptides and unique three dimensional structure that is affected by nucleotide sequence. The structure exclusively influences aptamers’ bindings with its
Nasrin Yazdanpanahi, Mehrdad Hashemi, Abolfazl Movafagh   +2 more
doaj   +3 more sources

A novel artificial intelligence-based approach for identification of deoxynucleotide aptamers.

PLoS Computational Biology, 2021
The selection of a DNA aptamer through the Systematic Evolution of Ligands by EXponential enrichment (SELEX) method involves multiple binding steps, in which a target and a library of randomized DNA sequences are mixed for selection of a single ...
Frances L Heredia, Abiel Roche-Lima, Elsie I Parés-Matos   +2 more
doaj   +1 more source

Analysis of interaction between RNA aptamer and protein using nucleotide analogs [PDF]

Nucleic Acids Symposium Series, 2000
Non-structural protein 3 (NS3) derived from Hepatitis C virus (HCV) is essential for viral proliferation and has two functional domains; trypsin-like serine protease and helicase. Recently we obtained three types of RNA aptamers (G9-I, -II and -III) bound to NS3 protease domain (delta NS3) by in vitro selection and confirmed their strong inhibition for
S, Sekiya, K, Fukuda, J, Hwang, N, Kakiuchi, K, Taira, I, Kusakabe, S, Nishikawa   +6 more
openaire   +2 more sources

Interrogating Aptamer Chemical Space Through Modified Nucleotide Substitution Facilitated by Enzymatic DNA Synthesis

ChemBioChem, 2023
AbstractChemical modification of aptamers is an important step to improve their performance and stability in biological media. This can be performed either during their identification (mod‐SELEX) or after the in vitro selection process (post‐SELEX).
Germain Niogret, Alix Bouvier‐Müller, Chiara Figazzolo, Jack M. Joyce, Frédéric Bonhomme, Patrick England, Olena Mayboroda, Riccardo Pellarin, Gilles Gasser, James H. R. Tucker, Julian A. Tanner, G. Paul Savage, Marcel Hollenstein   +12 more
openaire   +3 more sources

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Baugh, Bauke W. Dijkstra, Bing, Bock, Cao, Chayen, Convery, Doudna, Ellington, Friedmann, Garber, Hauke Smidt, Hermann, Hianik, Hoggan, Hollis, Horn, Huang, Huang, Hwang, Jiang, Johan Hekelaar, John van der Oost, Kaur, Ke, Kelly, Kikin, Krauss, Kwan, Laing, Lebruska, Lee, Long, Lupold, Macaya, Mark Levisson, Mascini, McPherson, Mehta, Miyakawa, Moorthy, Murai, Nix, Nomura, Orlova, Padmanabhan, Padmanabhan, Paige, Parisien, Poniková, Reinemann, Reinstein, Renault, Rivas, Rowsell, Ruigrok, Sekiya, Shum, Skrzypczak-Jankun, Snyder, Someya, Stoltenburg, Sugiyama, Sussman, Tereshko, Tuerk, Vincent J. B. Ruigrok, Wang, Wilson, Win, Wochner, Yan, Yee, Zuker   +73 more
core   +12 more sources

Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides

Nucleic Acids Research, 2020
AbstractGuanine-rich regions of the human genome can adopt non-canonical secondary structures. Their role in regulating gene expression has turned them into promising targets for therapeutic intervention. Ligands based on polyaromatic moieties are especially suitable for targeting G-quadruplexes utilizing their size complementarity to interact with the
Janez Plavec, Naoki Sugimoto, Shuntaro Takahashi, Hisae Tateishi-Karimata, Peter Podbevšek, Matic Kovačič   +5 more
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Structure–Activity Relationship Study of a Potent α‐Thrombin Binding Aptamer Incorporating Hexitol Nucleotides [PDF]

Chemistry – A European Journal, 2020
AbstractThe replacement of one or more nucleotide residues in the potent α‐thrombin‐binding aptamer NU172 with hexitol‐based nucleotides has been devised to study the effect of these substitutions on the physicochemical and functional properties of the anticoagulant agent.
De Fenza, Maria, Eremeeva, Elena, Troisi, Romualdo, Yang, Hui, Esposito, Anna, Sica, Filomena, Herdewijn, Piet, D'Alonzo, Daniele, Guaragna, Annalisa   +8 more
openaire   +5 more sources

Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases

ChemBioChem, 2020
AbstractOne of the pivotal steps in aptamer selection is the amplification of target‐specific oligonucleotides by thermophilic DNA polymerases; it can be a challenging task if nucleic acids possessing modified nucleotides are to be amplified. Hence, the identification of compatible DNA polymerase and modified nucleotide pairs is necessary for effective
Krisztina Percze, Tamás Mészáros
openaire   +2 more sources

Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Heliyon, 2023
The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide.
Zahra Sadat Moshref, Tahmineh Jalali, Razieh Rezaei Adriani, Elahe Soltati, Seyed Latif Mousavi Gargari   +4 more
doaj   +1 more source

Kinetic Exclusion Assay of Biomolecules by Aptamer Capture

Sensors, 2020
DNA aptamers are short nucleotide oligomers selected to bind a target ligand with affinity and specificity rivaling that of antibodies. These remarkable features recommend aptamers as candidates for analytical and therapeutic applications that ...
Mark H. Smith, Daniel Fologea
doaj   +1 more source