Results 11 to 20 of about 19,280 (250)

The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing -(1 -pentynyl)-2′-deoxyuridine [PDF]

Nucleic Acids Research, 1994
Combinatorial libraries of nucleic acids are developing into novel sources for lead compounds in drug development. In order to diversify the pool of ss DNA sequences, we have used a modified nucleotide, 5-(1-pentynyl)-2'-deoxyuridine, in place of thymidine in a random nucleic acid library and screened this library against human thrombin. Previously, we
John A. Latham, Richard Johnson, John J. Toole   +2 more
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Nucleotide Bias Observed with a Short SELEX RNA Aptamer Library [PDF]

Nucleic Acid Therapeutics, 2011
Systematic evolution of ligands by exponential enrichment (SELEX) is a powerful in vitro selection process used for over 2 decades to identify oligonucleotide sequences (aptamers) with desired properties (usually high affinity for a protein target) from randomized nucleic acid libraries.
William H, Thiel, Thomas, Bair, Kristina, Wyatt Thiel, Justin P, Dassie, William M, Rockey, Craig A, Howell, Xiuying Y, Liu, Adam J, Dupuy, Lingyan, Huang, Richard, Owczarzy, Mark A, Behlke, James O, McNamara, Paloma H, Giangrande   +12 more
openaire   +2 more sources

A novel artificial intelligence-based approach for identification of deoxynucleotide aptamers.

PLoS Computational Biology, 2021
The selection of a DNA aptamer through the Systematic Evolution of Ligands by EXponential enrichment (SELEX) method involves multiple binding steps, in which a target and a library of randomized DNA sequences are mixed for selection of a single ...
Frances L Heredia, Abiel Roche-Lima, Elsie I Parés-Matos   +2 more
doaj   +1 more source

Analysis of interaction between RNA aptamer and protein using nucleotide analogs [PDF]

Nucleic Acids Symposium Series, 2000
Non-structural protein 3 (NS3) derived from Hepatitis C virus (HCV) is essential for viral proliferation and has two functional domains; trypsin-like serine protease and helicase. Recently we obtained three types of RNA aptamers (G9-I, -II and -III) bound to NS3 protease domain (delta NS3) by in vitro selection and confirmed their strong inhibition for
S, Sekiya, K, Fukuda, J, Hwang, N, Kakiuchi, K, Taira, I, Kusakabe, S, Nishikawa   +6 more
openaire   +2 more sources

Interrogating Aptamer Chemical Space Through Modified Nucleotide Substitution Facilitated by Enzymatic DNA Synthesis

ChemBioChem, 2023
AbstractChemical modification of aptamers is an important step to improve their performance and stability in biological media. This can be performed either during their identification (mod‐SELEX) or after the in vitro selection process (post‐SELEX).
Germain Niogret, Alix Bouvier‐Müller, Chiara Figazzolo, Jack M. Joyce, Frédéric Bonhomme, Patrick England, Olena Mayboroda, Riccardo Pellarin, Gilles Gasser, James H. R. Tucker, Julian A. Tanner, G. Paul Savage, Marcel Hollenstein   +12 more
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Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Baugh, Bauke W. Dijkstra, Bing, Bock, Cao, Chayen, Convery, Doudna, Ellington, Friedmann, Garber, Hauke Smidt, Hermann, Hianik, Hoggan, Hollis, Horn, Huang, Huang, Hwang, Jiang, Johan Hekelaar, John van der Oost, Kaur, Ke, Kelly, Kikin, Krauss, Kwan, Laing, Lebruska, Lee, Long, Lupold, Macaya, Mark Levisson, Mascini, McPherson, Mehta, Miyakawa, Moorthy, Murai, Nix, Nomura, Orlova, Padmanabhan, Padmanabhan, Paige, Parisien, Poniková, Reinemann, Reinstein, Renault, Rivas, Rowsell, Ruigrok, Sekiya, Shum, Skrzypczak-Jankun, Snyder, Someya, Stoltenburg, Sugiyama, Sussman, Tereshko, Tuerk, Vincent J. B. Ruigrok, Wang, Wilson, Win, Wochner, Yan, Yee, Zuker   +73 more
core   +12 more sources

Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides

Nucleic Acids Research, 2020
AbstractGuanine-rich regions of the human genome can adopt non-canonical secondary structures. Their role in regulating gene expression has turned them into promising targets for therapeutic intervention. Ligands based on polyaromatic moieties are especially suitable for targeting G-quadruplexes utilizing their size complementarity to interact with the
Janez Plavec, Naoki Sugimoto, Shuntaro Takahashi, Hisae Tateishi-Karimata, Peter Podbevšek, Matic Kovačič   +5 more
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Structure–Activity Relationship Study of a Potent α‐Thrombin Binding Aptamer Incorporating Hexitol Nucleotides [PDF]

Chemistry – A European Journal, 2020
AbstractThe replacement of one or more nucleotide residues in the potent α‐thrombin‐binding aptamer NU172 with hexitol‐based nucleotides has been devised to study the effect of these substitutions on the physicochemical and functional properties of the anticoagulant agent.
De Fenza, Maria, Eremeeva, Elena, Troisi, Romualdo, Yang, Hui, Esposito, Anna, Sica, Filomena, Herdewijn, Piet, D'Alonzo, Daniele, Guaragna, Annalisa   +8 more
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Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases

ChemBioChem, 2020
AbstractOne of the pivotal steps in aptamer selection is the amplification of target‐specific oligonucleotides by thermophilic DNA polymerases; it can be a challenging task if nucleic acids possessing modified nucleotides are to be amplified. Hence, the identification of compatible DNA polymerase and modified nucleotide pairs is necessary for effective
Krisztina Percze, Tamás Mészáros
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Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Heliyon, 2023
The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide.
Zahra Sadat Moshref, Tahmineh Jalali, Razieh Rezaei Adriani, Elahe Soltati, Seyed Latif Mousavi Gargari   +4 more
doaj   +1 more source