Results 111 to 120 of about 42,927 (266)

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

Advanced Science, Volume 12, Issue 29, August 7, 2025.
This work presents a strategy for engineering thermophilic Ago proteins to obtain wide catalytic temperature range and broad substrate spectrum. This strategy is applied to engineer PfAgo and TtdAgo, and the biochemical properties of the engineered PfAgo are systematically characterized. The study further elucidates the structural differences involved.
Longyu Wang, Xiaochen Xie, Fuyong Huang, Qiang Wei, Tianxin Cai, Na Yu, Shi Chen, Fei Wang, Wanping Chen, Chin‐yu Chen, Chunhua Li, Lixin Ma   +11 more
wiley   +1 more source

Structure and Function of Argonaute Proteins [PDF]

Structure, 2005
Argonaute (Ago) family proteins are multidomain proteins expressed in prokaryotic and eukaryotic organisms. In eukaryotes, Ago proteins are most well known for their roles in RNA silencing. In prokaryotes, the functions of Ago proteins are unknown, but based on their similarity to eukaryotic Ago proteins, they could be involved in nucleic acid-directed
openaire   +3 more sources

Analysis of wheat SAGE tags reveals evidence for widespread antisense transcription [PDF]

, 2008
BACKGROUND: Serial Analysis of Gene Expression (SAGE) is a powerful tool for genome-wide transcription studies. Unlike microarrays, it has the ability to detect novel forms of RNA such as alternatively spliced and antisense transcripts, without the need ...
Barker, Gary L A, Berry, Simon, Biggi, Gaia F, Coghill, Jane, Dunwell, Jim, Edwards, Keith J, Gibbings, George, Poole, Rebecca L, Werner, Kay   +8 more
core   +2 more sources

The Role of PIWI‐Interacting RNA in Urologic Carcinoma and Its Clinical Implications

Cancer Reports, Volume 8, Issue 8, August 2025.
ABSTRACT Piwi‐interacting RNA (piRNA) represents a class of small non‐coding RNA molecules, typically ranging in length from 18 to 35 nucleotides. These molecules are critically involved in the preservation of genomic integrity and the regulation of protein translation processes.
Jiajia Cai, Shirui Jiang, Dongdong Lu, Ghulam Murtaza, Mingbang Wang, Yuqing Li, Da Shi, Yingrui Li, Hu Fang, Song Wu   +9 more
wiley   +1 more source

Notes on the Argonaut [PDF]

The American Naturalist
THE Argonaut, or Paper-sailor, is familiar to all who live in seaports; its elegant form and delicate texture making it deservedly a favorite ornament for table or mantel; and certainly nothing can be more exquisite than a perfect specimen of one of the larger species.
openaire   +2 more sources

Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi

Nature Communications, 2017
While RNA interference is a highly conserved mechanism of gene regulation, how Argonaute-bound small RNAs are targeted for degradation is not well understood.
Paola Pisacane, Mario Halic
doaj   +1 more source

A robust machine learning model based on ribosomal‐subunit‐derived piRNAs for diagnostic potential of nonsmall cell lung cancer across multicentre, large‐scale of sequencing data

Clinical and Translational Medicine, Volume 15, Issue 8, August 2025.
NSCLC lacks robust noninvasive biomarkers. We curated 2050 invasive and noninvasive samples, integrating public and in‐house plasma/exosome data. A machine learning model using five tumour‐specific piRNA signatures formed the piRNA‐Based Tumour Probability Index (pi‐TPI), achieving AUC values up to .96, demonstrating strong potential for NSCLC ...
Zitong Gao, Masaki Nasu, Gehan Devendra, Ayman A. Abdul‐Ghani, Anthony J. Herrera, Jeffrey A. Borgia, Christopher W. Seder, Donna Lee Kuehu, Zhuokun Feng, Yu Chen, Ting Gong, Zao Zhang, Owen Chan, Hua Yang, Jianhua Yu, Yuanyuan Fu, Lang Wu, Youping Deng   +17 more
wiley   +1 more source

Argonaute bypasses cellular obstacles without hindrance during target search

Nature Communications, 2019
Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and ...
Tao Ju Cui, Misha Klein, Jorrit W. Hegge, Stanley D. Chandradoss, John van der Oost, Martin Depken, Chirlmin Joo   +6 more
doaj   +1 more source

A virulent strain of Deformed Wing Virus (DWV) of Honeybees (Apis mellifera) prevails after Varroa destructor-mediated, or in vitro, transmission [PDF]

, 2014
This work was supported by the Biotechnology and Biological Sciences Research Council, the Department for Environment, Food and Rural Affairs, the Natural Environment Research Council, the Scottish Government and the Wellcome Trust, under the Insect ...
Bull, James C., Burroughs, Nigel, Chandler, Dave, Evans, David J., Fannon, Jessica M., Mead, Andrew, Moore, Jonathan D., Ryabov, Eugene V., Wood, Graham R.   +8 more
core   +3 more sources

Exomeres From Adventitial Fibroblasts of Spontaneously Hypertensive Rats Promote Vascular Remodelling via Transferring Osteopontin

Journal of Extracellular Vesicles, Volume 14, Issue 8, August 2025.
ABSTRACT Vascular adventitial fibroblasts (VAFs) contribute to vascular remodelling in hypertension. However, the mechanisms by which VAFs regulate vascular smooth muscle cells (VSMCs) in vascular remodelling are not well known. Here we report the crucial roles of extracellular nanoparticles exomeres (EMs) derived from VAFs in promoting VSMCs ...
Jing‐Xiao Wang, Xiao‐Yu Xu, Hong‐Ke Dong, Yi‐Ming Wang, Min Dai, Bing Zhou, Yue‐Hua Li, Guo‐Qing Zhu, Xiao‐Qing Xiong   +8 more
wiley   +1 more source