Results 121 to 130 of about 30,324 (235)

Simulasi protein argonaute dalam pelarut air eksplisit [PDF]

, 2010
Terapi gen merupakan terobosan baru dalam dunia pengobatan. Salah satu mekanismenya adalah membungkam gen agar tidak mengekspresikan gen yang jelek, abnormal, atau menyebabkan penyakit.
Gianto, Agus
core  

4′‐ThioRNA Substitution and Sense‐Strand Segmentation for the Design of Functional Fully Modified siRNAs Targeting SARS‐CoV‐2

Chemistry – A European Journal, Volume 32, Issue 40, 27 October 2026.
4′‐ThioRNA was broadly tolerated across multiple positions in SARS‐CoV‐2‐targeting siRNAs, but full 4'‐thio modification of both strands caused a reduction in RNAi activity. Sense‐strand segmentation improved RNAi and antiviral activity, particularly when the nick was located at position 10 from the 5′‐end of the sense strand, thereby unlocking the ...
Yuhei Nogi, Jun Tsukimoto, Noriko Saito‐Tarashima, Takaaki Koma, Masako Nomaguchi, Noriaki Minakawa   +5 more
wiley   +1 more source

Argonaute proteins - Methods and protocols

, 2012
Before reading these few lines reviewing the Argonaute family proteins (what they are and what they do) I strongly advise the reader to go through both the greek myth and two posters: Production and actions of small RNAs by V.
Manuela Monti
core   +1 more source

Argonaute bypasses cellular obstacles without hindrance during target search

Nature Communications, 2019
Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and ...
Tao Ju Cui, Misha Klein, Jorrit W. Hegge, Stanley D. Chandradoss, John van der Oost, Martin Depken, Chirlmin Joo   +6 more
doaj   +1 more source

Quantitation of functional transfected miRNA mimic by deep sequencing of RNA from Argonaute immunoprecipitation.

, 2013
A) MDA-MB-231 cells were transfected with miR-200a, with scrambled control, or were untransfected, then subjected to UV-crosslinking and Argonaute immunoprecipitation followed by deep sequencing of the Argonaute-bound small RNA pool. The levels of miRNAs
Jan M. Szubert (287169), Gregory J. Goodall (287170), Cameron P. Bracken (287167), Daniel W. Thomson (287165)   +3 more
core   +1 more source

Asgard archaea defense systems and their roles in the origin of eukaryotic immunity

Nature Communications
Dozens of new antiviral systems have been recently characterized in bacteria. Some of these systems are present in eukaryotes and appear to have originated in prokaryotes, but little is known about these defense mechanisms in archaea.
Pedro Leão, Mary E. Little, Kathryn E. Appler, Daphne Sahaya, Emily Aguilar-Pine, Kathryn Currie, Ilya J. Finkelstein, Valerie De Anda, Brett J. Baker   +8 more
doaj   +1 more source

Drosha, Dicer-1 and Argonaute-1 in the desert locust: Phylogenetic analyses, transcript profiling and regulation during phase transition and feeding

, 2015
In this article, we identify and characterise the miRNA machinery components Drosha, Dicer-1 and Argonaute-1 of the desert locust. By means of phylogenetic analyses, we reveal important insights in the evolutionary context of these components.
Wynant, Niels, Verlinden, Heleen, Santos, Dulce, Vanden Broeck, Jozef, Subramanyam, Sudheendra Hebbar   +4 more
core   +1 more source

Search for the Argonaute protein that governs miRNA regulation in Dictyostelium discoideum

, 2021
MicroRNAs are small non-coding RNAs that regulate gene expression through RNA interference. These small RNAs enact gene silencing by forming a RNA-inducing silencing complex together with the effector protein Argonaute.
Åström, Miranda
core  

Detection of Argonaute (Ago) protein associated MiRNA by combining anti-ago antibody recognition with real-time PCR [PDF]

, 2014
MicroRNAs (miRNAs) are small molecules of noncoding RNA that range between ~19-22 nucleotides in length. In recent years, scientists have observed that these small RNA molecules exist in the extracellular environment within eukaryotic organisms ...
Coley, Brian D., NC DOCKS at The University of North Carolina at Greensboro   +1 more
core  

Transfer RNA and Origins of RNA Interference

Frontiers in Molecular Biosciences, 2021
Andrey Grigoriev
doaj   +1 more source