Results 191 to 200 of about 17,633 (227)

Activation of polyphosphoinositide metabolism at artificial maturation of Patella vulgata oocytes

Developmental Biology, 1992
The metabolism of polyphosphoinositides (PPI) has been investigated during the meiosis reinitiation of the oocytes of a prosobranch mollusk, the limpet Patella vulgata. Meiosis reinitiation which leads to germinal vesicle breakdown (GVBD) and metaphase-1 spindle formation was artificially induced by treating the prophase-blocked oocytes with 10 mM ...
B, Borg, G, De Renzis, P, Payan, B, Ciapa   +3 more
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Respiration of starfish oocytes during meiosis, fertilization, and artificial activation

Experimental Cell Research, 1974
Abstract Rates of oxygen consumption were measured in oocytes of the starfish Patiria miniata prior to and after the initiation of meiotic maturation in response to 1-methyladenine. No significant change in the rate of respiration was noted until after the completion of meiosis, at which point a two-fold increase in the rate of respiration was ...
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Culture conditions affect Ca2+ release in artificially activated mouse and human oocytes

Reproduction, Fertility and Development, 2018
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of
Yuechao, Lu, Davina, Bonte, Minerva, Ferrer-Buitrago, Mina, Popovic, Jitesh, Neupane, Margot, Van der Jeught, Luc, Leybaert, Petra, De Sutter, Björn, Heindryckx   +8 more
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Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes

2022
Parthenogenesis is a common reproductive strategy among lower animals that involves the development of an embryo from an oocyte, without any contribution from spermatozoon. This phenomenon does not occur naturally in placental mammals. However, the mammalian oocytes can be artificially activated in vitro using mechanical, electrical, and chemical ...
Daphne Norma, Crasta, Satish Kumar, Adiga, Nagarajan, Kannan, Guruprasad, Kalthur   +3 more
openaire   +2 more sources

Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI

Journal of Mammalian Ova Research, 2015
Abstract: At our clinic, a successfully fertilized human oocyte is defined as one with two polar bodies and two pronuclei (PN) 18 h after ICSI, but when some oocytes do not form PN. Recently, a successful pregnancy and live birth following artificial oocyte activation (AOA; using Ca2+ ionophore) of ICSI-treated one-day-old unfertilized oocytes was ...
Masahiro Sakurai, Shinichi Watanabe, Toyomi Tanaka, Rie Matsunaga, Naoko Yamanaka, Minako Kamihata, Chikako Kani, Akiko Kuwahata, Masanori Ochi, Toshitaka Horiuchi   +9 more
openaire   +1 more source

Conditions for in vitro maturation and artificial activation of ferret oocytes.

Biology of reproduction, 2002
The ferret represents an attractive species for animal modeling of lung diseases because of the similarity between ferret and human lung biology and its relatively small size and short gestation time. In an effort to establish experimental protocols necessary for cloning ferrets, optimized conditions for in vitro maturation and artificial activation of
Ziyi, Li, Qinshi, Jiang, Maryam, Rezaei Sabet, Yulong, Zhang, Teresa C, Ritchie, John F, Engelhardt   +5 more
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Artificial Oocyte Activation to Improve Blastocyst Development

Reproductive BioMedicine Online, 2022
S. Montgomery, S. Boardman, A. Campbell
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Comparison of clinical artificial oocyte activation protocols on mouse egg activation and embryo development

Reproduction
In briefArtificial oocyte activation (AOA) with Ca2+ ionophores is a valuable tool in assisted reproduction but may affect cellular events critical for embryo development. This study shows the impact of different AOA protocols on embryonic development efficiency and meiotic progression.AbstractAOA with Ca2+ ionophores is an experimental procedure that ...
Lucas N González, Valeria Sulzyk, Patricia S Cuasnicú, Débora J Cohen   +3 more
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Effect of culture conditions on artificial activation of porcine oocytes matured in vitro

Reproduction, Fertility and Development, 1996
The effects of culture media used and culture period for in vitro maturation of porcine oocytes on their subsequent response to chemical and electrical activation, were investigated. Activated oocytes were identified by the presence of a pronucleus(ei) or cleavage.
N, Yamauchi, H, Sasada, S, Sugawara, T, Nagai   +3 more
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Effect of high fat diet on artificial oocyte activation following superovulation in mice

Zygote, 2015
SummaryThe aim of the present study was to determine the effects of increased dietary intake and high fat diet (HFD) in mice on artificial oocyte activation by using puromycin or roscovitine. Six-week-old mice were fed as either a control diet group, an increased dietary intake group or an HFD group for 4 weeks.
Daisuke, Yamamoto, Toshiyuki, Yasui, Chika, Kobayashi, Takane, Kitazato, Takeshi, Iwasa, Minoru, Irahara   +5 more
openaire   +2 more sources