Results 201 to 210 of about 17,633 (227)

Artificial oocyte activation with CALCIUM IONOPHORE (A+23187) following ICSI fertilization failure

Fertility and Sterility, 2015
masturbation following 2-5 days abstinence and subjected to computer-assisted sperm analysis (CASA), Kremer penetration testing, calcium response recording (FLUOstar), PLCz immunocytochemistry (anti-human PLCz; Cova-Lab UK), and electrophysiology studies using single-cell patch clamping(4).
A. Sdrigotti, G.J. Rey Valzacchi, F.A. Leocata Nieto, V.E. Canada   +3 more
openaire   +1 more source

Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes

Genes to Cells
Abstract In eukaryotes, DNA is housed within the cell nucleus. Molecules required for the formation of a nucleus have been identified using in vitro systems with frog egg extracts and in vivo imaging of somatic cells. However, little is known about the physicochemical factors and conditions required for nuclear formation in mouse ...
Nao Yonezawa, Tomoko Shindo, Haruka Oda, Hiroshi Kimura, Yasushi Hiraoka, Tokuko Haraguchi, Kazuo Yamagata   +6 more
openaire   +2 more sources

Intracellular Ca2+ during fertilization and artificial activation in mouse oocytes.

Zhongguo yao li xue bao = Acta pharmacologica Sinica, 1999
To study the mechanism of oocyte activation in mammals.Mouse oocytes arrested at metaphase of the second meiotic division were loaded with Fura 2-AM and then activated with ethanol, calcimycin, electric pulse or fertilization. Intracellular free Ca2+ during activation were measured by Spex AR-CM-MIC cation system.
M Q, Deng, B Q, Fan
openaire   +1 more source

[A control study of artificial oocyte activation in the same period].

Zhonghua yi xue za zhi, 2017
Objective: To compare the fertility rate and embryo outcome between normal fertilization and the use of Calcium ionophore A23187 on the same period of the same cycle of human ovum for artificial activation. Methods: Patients who conducted the intracytoplasmic sperm injection (ICSI) cycle in vitro fertilization assisted reproductive from January 2015 to
D Y, Hao, R, Zhang, X, Zhang, C Y, Shen, Y, Yang, W B, Wu, Y L, Liu, J, Liu, J R, Zhang, X L, Wang   +9 more
openaire   +1 more source

Artificial Oocyte Activation After ICSI

2021
openaire   +1 more source

Artificial oocyte activation with ionomycin compared with A23187 among patients at risk of failed or impaired fertilization

Reproductive BioMedicine Online, 2023
Xiaoyan Liang
exaly  

Artificial oocyte activation in ICSI cycles using testicular spermatozoa

Fertility and Sterility, 2016
A. Sdrigotti, F.A. Leocata Nieto, L. Dacharry, G. Rey Valzacchi   +3 more
openaire   +1 more source

Use of phospholipase C zeta analysis to identify candidates for artificial oocyte activation: a case series of clinical pregnancies and a proposed algorithm for patient management

Fertility and Sterility, 2020
Xin Meng, Pedro Melo, Ginny Mounce
exaly  

Artificial Activation of Mammalian Oocytes for Cloning

2014
Takuya Wakai, Junya Ito, Rafael A. Fissore   +2 more
openaire   +1 more source

Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis

Fertility and Sterility, 2017
Srdjan Saso,, Mrcog, Mrcs, Benjamin Jones, Timothy Bracewell-Milnes   +2 more
exaly