Results 31 to 40 of about 11,891 (170)

Beyond Autophagy: The Expanding Roles of ATG8 Proteins

Trends in Biochemical Sciences, 2021
The ATG8 family proteins are critical players in autophagy, a cytoprotective process that mediates degradation of cytosolic cargo. During autophagy, ATG8s conjugate to autophagosome membranes to facilitate cargo recruitment, autophagosome biogenesis, transport, and fusion with lysosomes, for cargo degradation.
Nieto-Torres, Jose L, Leidal, Andrew M, Debnath, Jayanta, Hansen, Malene   +3 more
openaire   +4 more sources

In vitro systems for Atg8 lipidation

Methods, 2015
Macroautophagy is a major bulk degradation pathway for cytoplasmic material in eukaryotic cells. During macroautophagy, double membrane-bound organelles called autophagosomes are formed in a de novo manner. In the course of their formation autophagosomes capture cytoplasmic material, which is subsequently degraded upon fusion with the lysosomal system ...
Bettina Zens, Justyna Sawa-Makarska, Sascha Martens   +2 more
openaire   +2 more sources

A role for Atg8–PE deconjugation in autophagosome biogenesis [PDF]

Autophagy, 2012
Formation of the autophagosome is likely the most complex step of macroautophagy, and indeed it is the morphological and functional hallmark of this process; accordingly, it is critical to understand the corresponding molecular mechanism. Atg8 is the only known autophagy-related (Atg) protein required for autophagosome formation that remains associated
Nair, Usha, Yen, Wei-Lien, Mari, Muriel, Cao, Yang, Xie, Zhiping, Baba, Misuzu, Reggiori, Fulvio, Klionsky, Daniel J   +7 more
openaire   +3 more sources

Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein

eLife, 2018
The ubiquitin-like protein Atg8, in its lipidated form, plays central roles in autophagy. Yet, remarkably, Atg8 also carries out lipidation-independent functions in non-autophagic processes. How Atg8 performs its moonlighting roles is unclear.
Xiao-Man Liu, Akinori Yamasaki, Xiao-Min Du, Valerie C Coffman, Yoshinori Ohsumi, Hitoshi Nakatogawa, Jian-Qiu Wu, Nobuo N Noda, Li-Lin Du   +8 more
doaj   +1 more source

Characterization of the autophagy marker protein Atg8 reveals atypical features of autophagy in Plasmodium falciparum. [PDF]

PLoS ONE, 2014
Conventional autophagy is a lysosome-dependent degradation process that has crucial homeostatic and regulatory functions in eukaryotic organisms. As malaria parasites must dispose a number of self and host cellular contents, we investigated if autophagy ...
Rahul Navale, Atul, Aparna Devi Allanki, Puran Singh Sijwali   +3 more
doaj   +1 more source

PI3P binding by Atg21 organises Atg8 lipidation [PDF]

The EMBO Journal, 2015
Autophagosome biogenesis requires two ubiquitin-like conjugation systems. One couples ubiquitin-like Atg8 to phosphatidylethanolamine, and the other couples ubiquitin-like Atg12 to Atg5. Atg12~Atg5 then forms a heterodimer with Atg16. Membrane recruitment of the Atg12~Atg5/Atg16 complex defines the Atg8 lipidation site.
Juris, Lisa, Montino, Marco, Rube, Peter, Schlotterhose, Petra, Thumm, Michael, Krick, Roswitha   +5 more
openaire   +3 more sources

A unifying model for the role of the ATG8 system in autophagy

Journal of Cell Science, 2022
ABSTRACT The formation of autophagosomes and their fusion with lysosomes are key events that underpin autophagic degradation of cargoes. The core ATG8 system, which consists of the ATG8 family of ubiquitin-like proteins and the machineries that conjugate them onto autophagosomal membranes, are among the most-studied autophagy components.
Thanh Ngoc Nguyen, Michael Lazarou
openaire   +2 more sources

AlphaFold2-multimer guided high-accuracy prediction of typical and atypical ATG8-binding motifs

PLoS Biology, 2023
Macroautophagy/autophagy is an intracellular degradation process central to cellular homeostasis and defense against pathogens in eukaryotic cells. Regulation of autophagy relies on hierarchical binding of autophagy cargo receptors and adaptors to ATG8 ...
Tarhan Ibrahim, Virendrasinh Khandare, Federico Gabriel Mirkin, Yasin Tumtas, Doryen Bubeck, Tolga O. Bozkurt   +5 more
doaj   +2 more sources

Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase

BMC Biology, 2021
Background The vacuole/lysosome is the final destination of autophagic pathways, but can also itself be degraded in whole or in part by selective macroautophagic or microautophagic processes.
Cheng-Wen He, Xue-Fei Cui, Shao-Jie Ma, Qin Xu, Yan-Peng Ran, Wei-Zhi Chen, Jun-Xi Mu, Hui Li, Jing Zhu, Qingqiu Gong, Zhiping Xie   +10 more
doaj   +1 more source

Infection Dynamics of ATG8 in Leishmania: Balancing Autophagy for Therapeutics

Molecules, 2022
In many regions of the world, Leishmaniasis is a cause of substantial mortality and ailment. Due to impediment in available treatment, development of novel and effective treatments is indispensable.
Vrushali Guhe, Farah Anjum, Alaa Shafie, Md Imtaiyaz Hassan, Visweswara Rao Pasupuleti, Shailza Singh   +5 more
doaj   +1 more source