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An Analysis of the Biotin–(Strept)avidin System in Immunoassays: Interference and Mitigation Strategies [PDF]
An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer +1 more
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Selective Targeting of Proteins by Hybrid Polyoxometalates: Interaction Between a Bis-Biotinylated Hybrid Conjugate and Avidin [PDF]
The Keggin-type polyoxometalate [γ-SiW10O36]8− was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique were ...
Valeria A. Zamolo +12 more
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Construction of chimeric dual-chain avidin by tandem fusion of the related avidins. [PDF]
BACKGROUND: Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction.
Tiina A Riihimäki +6 more
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Electrochemical sensing of biotin-avidin interaction was investigated on gold electrode modified with 3-mercaptopropanoic acid (MPA) successively co-assembled with cysteamine functionalized silver nanoparticles and biotin or avidin.
Nirgaman Bage, Pradip Kar
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Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis [PDF]
Impaired endothelialization of endovascular stents has been established as a major cause of in-stent restenosis and late stent thrombosis. Attempts to enhance endothelialization of inner stent surfaces by pre-seeding the stents with endothelial cells in ...
Ivan S. Alferiev +7 more
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Fool's gold standard? Immunoperoxidase staining with the mouse monoclonal antibody (Clone 22C11) for detecting axonal pathology after traumatic brain injury [PDF]
Guoxiang Xiong +3 more
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AVIDIN. 2. PURIFICATION AND COMPOSITION [PDF]
MD Melamed, NM GREEN
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The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the
Yanchao Lyu +4 more
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Pedestal High-Contrast Gratings for Biosensing
High-contrast gratings (HCG) are an excellent candidate for label-free detection of various kinds of biomarkers because they exhibit sharp and sensitive optical resonances.
Leonid Yu. Beliaev +8 more
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The challenge of effective integration and use of artificial organelles with orthogonal‐responsive membranes and their communication in eukaryotic protocells is to understand the intrinsic membrane characteristics.
Xueyi Wang +7 more
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