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An Analysis of the Biotin–(Strept)avidin System in Immunoassays: Interference and Mitigation Strategies [PDF]

open access: goldCurrent Issues in Molecular Biology, 2023
An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer   +1 more
doaj   +2 more sources

Selective Targeting of Proteins by Hybrid Polyoxometalates: Interaction Between a Bis-Biotinylated Hybrid Conjugate and Avidin [PDF]

open access: yesFrontiers in Chemistry, 2018
The Keggin-type polyoxometalate [γ-SiW10O36]8− was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique were ...
Valeria A. Zamolo   +12 more
doaj   +4 more sources

Construction of chimeric dual-chain avidin by tandem fusion of the related avidins. [PDF]

open access: yesPLoS ONE, 2011
BACKGROUND: Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction.
Tiina A Riihimäki   +6 more
doaj   +7 more sources

Electrochemical sensing of biotin-avidin interaction on gold electrode modified by silver nanoparticles through covalent co-assembling

open access: goldSensors International, 2022
Electrochemical sensing of biotin-avidin interaction was investigated on gold electrode modified with 3-mercaptopropanoic acid (MPA) successively co-assembled with cysteamine functionalized silver nanoparticles and biotin or avidin.
Nirgaman Bage, Pradip Kar
doaj   +2 more sources

Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis [PDF]

open access: greenScientific Reports, 2022
Impaired endothelialization of endovascular stents has been established as a major cause of in-stent restenosis and late stent thrombosis. Attempts to enhance endothelialization of inner stent surfaces by pre-seeding the stents with endothelial cells in ...
Ivan S. Alferiev   +7 more
doaj   +2 more sources

The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation

open access: yesNanomaterials, 2021
The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the
Yanchao Lyu   +4 more
doaj   +1 more source

Pedestal High-Contrast Gratings for Biosensing

open access: yesNanomaterials, 2022
High-contrast gratings (HCG) are an excellent candidate for label-free detection of various kinds of biomarkers because they exhibit sharp and sensitive optical resonances.
Leonid Yu. Beliaev   +8 more
doaj   +1 more source

Artificial Organelles with Orthogonal‐Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes

open access: yesAdvanced Science, 2021
The challenge of effective integration and use of artificial organelles with orthogonal‐responsive membranes and their communication in eukaryotic protocells is to understand the intrinsic membrane characteristics.
Xueyi Wang   +7 more
doaj   +1 more source

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