A New method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte [PDF]
Introduction: We propose a new method for the selective labeling, isolation and electrophoretic analysis of the Plasmodium falciparum protein exposed on the erythrocyte cell surface.
Ferru, Emanuela +2 more
core
ISOLATION AND PURIFICATION OF AVIDIN FROM EGG WHITE BY IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
Lysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme in pure form by immobilized metal affinity chromatography column (1.5 x 10cm) loaded with copper ions ...
Shalan Alwan Al-Mashikhi
doaj +1 more source
Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery
Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues.
Chenzhen Zhang +3 more
doaj +1 more source
Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help. [PDF]
Past work has shown that a subset of effector T cells with unique characteristics could abrogate hapten- or antigen-induced tolerance, and the reconstitution of this immune response has been termed contrasuppression.
Aicher, WK +6 more
core
Abstract Autonomic nerves are crucial in cardiac function and pathology. However, data on the distribution of cholinergic and noradrenergic nerves in normal and pathologic human hearts is lacking. Nonfailing donor hearts were pressure‐perfusion fixed, imaged, and dissected. Left ventricular cardiomyopathy samples were also obtained.
Peter Hanna +16 more
wiley +1 more source
Background The quantification of surface groups attached to non-woven fibers is an important step in developing nanofiber biosensing detection technologies.
Magnone Joshua +4 more
doaj +1 more source
Microtopography enhances intestinal drug absorption via Piezo1‐mediated tight junction modulation
Microtopography‐driven enhancement of oral peptide drug delivery by activating Piezo1 channels significantly improves paracellular permeability and bioavailability, offering a novel approach for optimizing macromolecule absorption in pharmaceutical formulations.
Yu Hu +12 more
wiley +1 more source
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on ...
Robert H. Batchelor +3 more
doaj +1 more source
Adipocytes in Aortic Stenosis: Association With Clinical and Morphological Indices
ABSTRACT Recently, great attention has been given to understanding the new pathogenetic mechanisms underlying aortic stenosis (AS). The study aims to understand the role of mature adipocytes in AS and their association with histologic, clinical, and echocardiographic data, an area previously overlooked in AS research.
Elena Zoico +9 more
wiley +1 more source
Background A chicken egg contains several biotin-binding proteins (BBPs), whose complete DNA and amino acid sequences are not known. In order to identify and characterise these genes and proteins we studied chicken cDNAs and genes available in the NCBI ...
Nordlund Henri R +5 more
doaj +1 more source

