Results 81 to 90 of about 90,586 (267)
BioTechniquesAvidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin ...
Sonia Mukherjee +3 more
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Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter
BioTechniques, 2004 Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide
Arthur Karlin +3 more
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Exploration, EarlyView.Targeting eEF1A1 with baicalein disrupts the eEF1A1‐Prdx4 interaction to prevent RNF14‐mediated ubiquitination and degradation of Prdx4. This stabilizes Prdx4 to suppress NF‐κB, thereby alleviating sepsis‐induced acute lung injury. ABSTRACT Sepsis is a systemic inflammatory response syndrome triggered by an uncontrolled host response to infection ...
Jing Chang +9 more
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ISOLATION AND PURIFICATION OF AVIDIN FROM EGG WHITE BY IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
The Iraqi Journal of Veterinary Medicine, 1996 Lysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme in pure form by immobilized metal affinity chromatography column (1.5 x 10cm) loaded with copper ions ...
Shalan Alwan Al-Mashikhi
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Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery
MethodsX, 2020 Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues.
Chenzhen Zhang +3 more
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The use of avidin-biotin interaction in immunoenzymatic techniques.
, 1979 Biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested.
S Avrameas, T Ternynck, J L Guesdon
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Interdisciplinary Medicine, EarlyView.The graphical abstract outlines a novel multifunctional ultrasound contrast agent for the diagnosis and treatment of ischemic diseases, along with innovative strategies to enhance its targeting efficiency. Abstract Ischemic diseases, such as myocardial infarction, stroke, and peripheral ischemia, are leading causes of death and disability worldwide ...
Yangyang Zhou +3 more
wiley +1 more source
Journal of Nanobiotechnology, 2011 Background The quantification of surface groups attached to non-woven fibers is an important step in developing nanofiber biosensing detection technologies.
Magnone Joshua +4 more
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Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation [PDF]
, 2016 Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine.
Firakania, Cicia +3 more
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The Kaohsiung Journal of Medical Sciences, EarlyView.ABSTRACT This study aimed to elucidate the role of the RNA‐binding protein embryonic lethality abnormal vision‐like protein 1 (ELAVL1) in the pathogenesis of allergic rhinitis (AR), specifically by investigating its potential to promote T helper 2 (Th2) cell differentiation by regulating the stability of carboxypeptidase A3 (CPA3) mRNA.
Meng‐Ya Liu +2 more
wiley +1 more source