Biotin, or vitamin B7, is essential for metabolic reactions. It must be obtained from external sources such as food and biotin/vitamin supplements because it is not biosynthesized by mammals.
Fatema Kaladari +4 more
doaj +1 more source
To evaluate point-of-care testing (POCT) for the potential early detection of biomarkers of Parkinson’s disease, a systematic investigation of portable and low-cost platforms is performed based on the Proton-enzyme-linked immunosorbent assay (Proton ...
Chia-Ming Yang +3 more
doaj +1 more source
Synthesis and Cytoprotective Characterization of 8-Hydroxyquinoline Betti Products
The 8-hydroxyquinoline pharmacophore scaffold has been shown to possess a range of activities as metal chelation, enzyme inhibition, cytotoxicity, and cytoprotection.
Iván Kanizsai +6 more
doaj +1 more source
Protein-mediated dethreading of a biotin-functionalised pseudorotaxane [PDF]
In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques.
Caldwell, S.T. +11 more
core +3 more sources
Synthesis of N-peptide-6-amino-D-luciferin Conjugates
A general strategy for the synthesis of N-peptide-6-amino-D-luciferin conjugates has been developed. The applicability of the strategy was demonstrated with the preparation of a known substrate, N-Z-Asp-Glu-Val-Asp-6-amino-D-luciferin (N-Z-DEVD-aLuc).
Anita K. Kovács +8 more
doaj +1 more source
Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system [PDF]
Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody ...
Andrew Thompsett +22 more
core +1 more source
Effects of Biotin-Avidin Interactions on Hydrogel Swelling
Affinity interactions between the small molecule biotin and the protein avidin have been used extensively to functionalize biomaterials. More recently, researchers have leveraged the changes in biotin-avidin affinity that occur upon biotin conjugation to
Talaial B. Alina +3 more
doaj +1 more source
Charge based intra-cartilage delivery of single dose dexamethasone using Avidin nano-carriers suppresses cytokine-induced catabolism long term [PDF]
Objective: Avidin exhibits ideal characteristics for targeted intra-cartilage drug delivery: its small size and optimal positive charge enable rapid penetration through full-thickness cartilage and electrostatic binding interactions that give long half ...
Bajpayee, Ambika Goel +3 more
core +1 more source
A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk [PDF]
Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological ...
Brocchi, Emiliana +7 more
core +4 more sources
Purification and crystallization of avidin [PDF]
An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1μg of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx.
N M, Green, E J, Toms
openaire +2 more sources

