Results 11 to 20 of about 132,737 (315)

Construction of Chimeric Dual-Chain Avidin by Tandem Fusion of the Related Avidins [PDF]

open access: yesPLoS ONE, 2011
Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction. Avidin is a homotetrameric protein, which promotes its modification to symmetrical entities.
Thomas K.M. Nyholm   +8 more
openaire   +8 more sources

Leishmania Antigen Nonspecific Binding to Avidin in Biotin-Avidin Immunoassays [PDF]

open access: greenClinical Diagnostic Laboratory Immunology, 2000
One of the most prevalent uses of avidin-biotin technology in recent years has been their use in immunoassays. In many cases, the signal is enhanced 10-fold or more, owing to the four biotin-binding sites of avidin and the multiple biotinyl groups on the derivatized antibody ([4][1], [5][2 ...
M. Portús, S. Agudelo
openaire   +5 more sources

The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation

open access: yesNanomaterials, 2021
The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the
Yanchao Lyu   +4 more
doaj   +1 more source

INVESTIGATIONS ON THE STABILITY OF AVIDIN

open access: hybridJournal of Biological Chemistry, 1942
Catharine S. Rose   +2 more
openaire   +3 more sources

A Systematic Study and Potential Limitations of Proton-ELISA Platform for α-Synuclein Antigen Detection

open access: yesChemosensors, 2021
To evaluate point-of-care testing (POCT) for the potential early detection of biomarkers of Parkinson’s disease, a systematic investigation of portable and low-cost platforms is performed based on the Proton-enzyme-linked immunosorbent assay (Proton ...
Chia-Ming Yang   +3 more
doaj   +1 more source

Biotinylated Quinone as a Chemiluminescence Sensor for Biotin-Avidin Interaction and Biotin Detection Application

open access: yesSensors, 2023
Biotin, or vitamin B7, is essential for metabolic reactions. It must be obtained from external sources such as food and biotin/vitamin supplements because it is not biosynthesized by mammals.
Fatema Kaladari   +4 more
doaj   +1 more source

Pedestal High-Contrast Gratings for Biosensing

open access: yesNanomaterials, 2022
High-contrast gratings (HCG) are an excellent candidate for label-free detection of various kinds of biomarkers because they exhibit sharp and sensitive optical resonances.
Leonid Yu. Beliaev   +8 more
doaj   +1 more source

An Analysis of the Biotin–(Strept)avidin System in Immunoassays: Interference and Mitigation Strategies

open access: yesCurrent Issues in Molecular Biology, 2023
An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer   +1 more
doaj   +1 more source

Synthesis of N-peptide-6-amino-D-luciferin Conjugates

open access: yesFrontiers in Chemistry, 2018
A general strategy for the synthesis of N-peptide-6-amino-D-luciferin conjugates has been developed. The applicability of the strategy was demonstrated with the preparation of a known substrate, N-Z-Asp-Glu-Val-Asp-6-amino-D-luciferin (N-Z-DEVD-aLuc).
Anita K. Kovács   +8 more
doaj   +1 more source

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