Results 41 to 50 of about 136,222 (342)

Immunostaining of skeletal tissues [PDF]

open access: yes, 2011
Peer ...
De Bari, Cosimo, Kurth, Tobias B
core   +1 more source

Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin.

open access: yesExperimental Biology and Medicine, 1964
SummaryAn assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable.
R D, WEI, L D, WRIGHT
openaire   +2 more sources

Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum

open access: yesBiosensors, 2012
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the ...
Mathieu Lazerges   +3 more
doaj   +1 more source

Synthesis and In Vitro Cytotoxicity and Antibacterial Activity of Novel 1,2,3-Triazol-5-yl-Phosphonates

open access: yesMolecules, 2020
Novel 1,2,3-triazol-5-yl-phosphonates were prepared by the copper(I)-catalyzed domino reaction of phenylacetylene, organic azides and dialkyl phosphites.
Anna Tripolszky   +6 more
doaj   +1 more source

Adhesion-Induced Lateral Phase Separation in Membranes [PDF]

open access: yes, 2000
Adhesion between membranes is studied using a phenomenological model, where the inter-membrane distance is coupled to the concentration of sticker molecules on the membranes.
Andelman, David, Komura, Shigeyuki
core   +2 more sources

Avidin acylation prevents the complement-dependent lysis of avidin-carrying erythrocytes [PDF]

open access: yesBiochemical Journal, 1991
Non-covalent binding of avidin to biotinylated erythrocytes results in complement-dependent haemolysis. Biotinylated erythrocytes, as well as native cells, are not lysed by complement. Complement activation requires a tight contact between avidin and the erythrocyte membrane, since avidin does not in itself activate complement and does not inhibit ...
V R, Muzykantov   +2 more
openaire   +2 more sources

Mutations in human dynamin block an intermediate stage in coated vesicle formation [PDF]

open access: yes, 1993
The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to ...
Damke, Hanna   +5 more
core   +3 more sources

Thermochemistry of the Avidin‐Biotin Reaction [PDF]

open access: yesEuropean Journal of Biochemistry, 1972
The enthalpy for the binding of biotin to avidin has been determined at 25, 34 and 43°C (pH 9, ammonium acetate buffer): ΔH(25°C) =–22.5 ± 0.1 kcal/mol biotin; ΔCp=–237 ± 12 cal × mol biotin−1× K−1.Solubilities and enthalpies of solution of biotin in water and in ethanol were determined at 25°C. The transfer process for biotin between water and ethanol
J, Suurkuusk, I, Wadsö
openaire   +2 more sources

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

open access: yesAdvanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer   +7 more
wiley   +1 more source

Affinity of Avidin for Biocytin

open access: yesExperimental Biology and Medicine, 1950
SummaryAvidin has a greater affinity for biotin than for biocytin. The affinity ratio for biocytin, as previously defined, is about 4.
L D, WRIGHT   +4 more
openaire   +2 more sources

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