Results 71 to 80 of about 136,886 (329)

A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

BioTechniques
Avidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin ...
Sonia Mukherjee, Pierre Leblanc, Mark C Poznansky, Ann E Sluder   +3 more
doaj   +1 more source

Avidin related protein 2 shows unique structural and functional features among the avidin protein family

BMC Biotechnology, 2005
Background The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in ...
Salminen Tiina A, Johnson Mark S, Nyholm Thomas KM, Kulomaa Tuomas, Hörhä Jarno, Halling Katrin K, Kidron Heidi, Määttä Juha AE, Hytönen Vesa P, Kulomaa Markku S, Airenne Tomi T   +10 more
doaj   +1 more source

Microparticle assembly pathways on lipid membranes [PDF]

, 2017
Understanding interactions between microparticles and lipid membranes is of increasing importance, especially for unraveling the influence of microplastics on our health and environment.
Heinrich, Doris, Kraft, Daniela J., van der Wel, Casper   +2 more
core   +3 more sources

Engineered GM1 Intersects Between Mitochondrial and Synaptic Pathways to Ameliorate ALS Pathology

Advanced Science, EarlyView.
Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease driven by genetic and molecular disruptions affecting energy balance, protein homeostasis, and stress responses in nerve cells. Studies using human and rodent models identified convergent defects in mitochondria and synaptic function.
Federica Pilotto, Tristan Dellazizzo Toth, Silvano Bond, Alexander Schmitz, Rim Diab, Sara Y. Ngo Tenlep, Brian Mooney, Silvia Erni, Martina Schobesberger, Olivier Scheidegger, Camille Peitsch, Smita Saxena   +11 more
wiley   +1 more source

Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

BMC Biotechnology, 2011
Background Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications.
Kulomaa Markku S, Hinterdorfer Peter, Ebner Andreas, Määttä Juha AE, Nordlund Henri R, Rangl Martina, Hiltunen Soili, Riihimäki Tiina A, Takkinen Kristiina, Hytönen Vesa P   +9 more
doaj   +1 more source

Exosome-Based Cartilage-Targeted Delivery System: Strategies and Applications. [PDF]

Small Sci
Construction strategies and applications of cartilage‐targeting exosomes. With the ageing of the global population, cartilage‐related diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IVDD), have increasingly become significant social problems threatening human health. Therefore, targeted therapy for cartilage is becoming more
Wang Y, Chen B, Zhu H, Sun Z.
europepmc   +2 more sources

Leishmania Antigen Nonspecific Binding to Avidin in Biotin-Avidin Immunoassays [PDF]

Clinical Diagnostic Laboratory Immunology, 2000
One of the most prevalent uses of avidin-biotin technology in recent years has been their use in immunoassays. In many cases, the signal is enhanced 10-fold or more, owing to the four biotin-binding sites of avidin and the multiple biotinyl groups on the derivatized antibody ([4][1], [5][2 ...
S, Agudelo, M, Portús
openaire   +2 more sources

Islet Amyloid Polypeptide Modelled to Simulate Diabetes Co‐Oligomerized with β‐Amyloid 1‐42 Reproducing the Pathological Cascade of Alzheimer's Disease in Human Cerebral Organoids

Advanced Science, EarlyView.
Human islet amyloid polypeptide (hIAPP) was used to mimic T2DM, and Aβ42‐hIAPP co‐oligomers were delivered into the human mature cerebral organoids (COs), which reproduce typical AD pathology and significant neuronal death more closely resembling that of AD patients.
Jin Yan, Zhimeng Tang, Yanyu Luo, Bin Liu, Xinxin Huang, Jun Wei, Feng Yue   +6 more
wiley   +1 more source

Adhesion-Induced Lateral Phase Separation in Membranes [PDF]

, 2000
Adhesion between membranes is studied using a phenomenological model, where the inter-membrane distance is coupled to the concentration of sticker molecules on the membranes.
Andelman, David, Komura, Shigeyuki
core   +2 more sources

Biomimetic Nanomedicine for Senescence‐Modulated Immune Activation Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma

Advanced Science, EarlyView.
A multifunctional mPDZM nanoplatform is developed in this study. mPDZM integrates chemotherapy‐induced senescence, selective senolysis, and STING‐mediated immune activation. mPDZM effectively clears senescent tumor cells, remodels the tumor immune microenvironment, and enhances antitumor T‐cell responses.
Shiji Fang, Liyun Zheng, Bin Lin, Jiale Chen, Dehai Hou, Yiming Ding, Mengzhu Han, Pan Qin, Mengyuan Wang, Xiaoju Guo, Yeyu Zhang, Gaofeng Shu, Fazong Wu, Jianfei Tu, Minjiang Chen, Zhongwei Zhao, Zhuang Liu, Jiansong Ji   +17 more
wiley   +1 more source