Results 81 to 90 of about 136,222 (342)

Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

BMC Biotechnology, 2011
Background Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications.
Kulomaa Markku S, Hinterdorfer Peter, Ebner Andreas, Määttä Juha AE, Nordlund Henri R, Rangl Martina, Hiltunen Soili, Riihimäki Tiina A, Takkinen Kristiina, Hytönen Vesa P   +9 more
doaj   +1 more source

USP11‐PGAM5 Axis Promotes Neurotoxic Astrocyte Reactivity by Aggravating the mtDNA‐cGAS‐STING Pathway After Intracerebral Hemorrhage

Advanced Science, EarlyView.
This study reveals that the deubiquitinase USP11 stabilizes PGAM5, triggering neurotoxic astrocyte transformation after intracerebral hemorrhage. PGAM5 promotes mPTP opening and Drp1 dephosphorylation, synergistically amplifying mtDNA leakage into the cytosol and leading to cGAS–STING hyperactivation.
Jiaqing He, Zijuan Qin, Yaning Cai, Haixiao Liu, Tinghao Wang, Qing Hu, Yanni Xu, Pan Yang, Xun Wu, Yan Qu, Wei Guo   +10 more
wiley   +1 more source

Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes [PDF]

, 1987
The existence of four subpopulations in resting porcine T lymphocytes is documented. In addition to the two known subpopulations which are typified by a mutually exclusive expression of either the CD8 or the CD4 differentiation antigen, CD4-CD8+ and CD4 ...
Bevan, Bielefeldt Ohmann, Blue, Blue, Davignon, Fazekas de St. Groth, Goverman, Jonjić, Lanier, Lanier, MacDonald, Mackay, Pescovitz, Pescovitz, Reinherz, Reinherz, Saalmüller, Scollay, Swain, Tumbleson   +19 more
core   +3 more sources

Stromal Cell‐Mast Cell Communication Orchestrates Anti‐Viral Immunity in the Meninges

Advanced Science, EarlyView.
This study delineates the spatial distribution and postnatal maturation of meningeal mast cells and uncovers their essential role in antiviral immunity. Using genetic models, mast cells are shown to promote CD8⁺ T cell infiltration and viral clearance via IL‐33 receptor signaling and synergistic activation by IL‐33 and ATP from infected stromal cells ...
Qingqing Li, Weijia Chen, Mengxue Sun, Xinbo Ni, Qishan Ran, Xiaoyu Hu, Fang Cao, Wenwen Zeng   +7 more
wiley   +1 more source

Biomimetic Nanomedicine for Senescence‐Modulated Immune Activation Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma

Advanced Science, EarlyView.
A multifunctional mPDZM nanoplatform is developed in this study. mPDZM integrates chemotherapy‐induced senescence, selective senolysis, and STING‐mediated immune activation. mPDZM effectively clears senescent tumor cells, remodels the tumor immune microenvironment, and enhances antitumor T‐cell responses.
Shiji Fang, Liyun Zheng, Bin Lin, Jiale Chen, Dehai Hou, Yiming Ding, Mengzhu Han, Pan Qin, Mengyuan Wang, Xiaoju Guo, Yeyu Zhang, Gaofeng Shu, Fazong Wu, Jianfei Tu, Minjiang Chen, Zhongwei Zhao, Zhuang Liu, Jiansong Ji   +17 more
wiley   +1 more source

Chemical linkage to injected tissues is a distinctive property of oxidized avidin.

PLoS ONE, 2011
We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups.
Rita De Santis, Anna Maria Anastasi, Angela Pelliccia, Antonio Rosi, Claudio Albertoni, Antonio Verdoliva, Fiorella Petronzelli, Valeria D'Alessio, Serenella Serani, Carlo Antonio Nuzzolo   +9 more
doaj   +1 more source

Deuterium Depletion Inhibits Cell Proliferation, RNA and Nuclear Membrane Turnover to Enhance Survival in Pancreatic Cancer

Cancer Control, 2021
The effects of deuterium-depleted water (DDW) containing deuterium (D) at a concentration of 25 parts per million (ppm), 50 ppm, 105 ppm and the control at 150 ppm were monitored in MIA-PaCa-2 pancreatic cancer cells by the real-time cell impedance ...
László G. Boros, Ildikó Somlyai, Beáta Zs. Kovács, László G. Puskás, Lajos I. Nagy, László Dux, Gyula Farkas, Gábor Somlyai   +7 more
doaj   +1 more source

A critical evaluation of direct electrical protein detection methods [PDF]

, 1991
During the last decennia many protein-related electrical phenomena have been studied and applied in a variety of measuring systems, from simple metal electrodes with adsorbed proteins to sophisticated systems with lipid bilayers.
Bergveld, P.
core   +2 more sources

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Biosensors
L-Lactate is an important bioanalyte in the food industry, biotechnology, and human healthcare. In this work, we report the development of a new L-lactate electrochemical biosensor based on the use of multiwalled carbon nanotubes non-covalently ...
Alejandro Tamborelli, Michael López Mujica, Marilla Amaranto, José Luis Barra, Gustavo Rivas, Agustina Godino, Pablo Dalmasso   +6 more
doaj   +1 more source

Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter

BioTechniques, 2004
Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide
Arthur Karlin, Chaojian Wang, Jing Li, Qiang Xu   +3 more
doaj   +1 more source