Results 81 to 90 of about 136,886 (329)

Three-dimensional structures of avidin and the avidin-biotin complex. [PDF]

Proceedings of the National Academy of Sciences, 1993
The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight binding of biotin.
O, Livnah, E A, Bayer, M, Wilchek, J L, Sussman   +3 more
openaire   +2 more sources

Antibody‐Empowered Nanomedicine for Precise Biomedical Applications

Advanced Science, EarlyView.
This review explores strategies for functionalizing nanoparticles with antibodies to construct antibody‐empowered nanomedicine. It discusses the classification of these nanomedicines based on antibody structure, with a specific focus on their biomedical applications in diagnostics, bioimaging, and therapeutics for various diseases.
Chen Chen, Xinglin Chen, Zhenhao Gao, Meng Sun, Yaozong Yu, Fang Lv, Qiujun Wang, Jinfeng Zhang   +7 more
wiley   +1 more source

Chemical linkage to injected tissues is a distinctive property of oxidized avidin.

PLoS ONE, 2011
We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups.
Rita De Santis, Anna Maria Anastasi, Angela Pelliccia, Antonio Rosi, Claudio Albertoni, Antonio Verdoliva, Fiorella Petronzelli, Valeria D'Alessio, Serenella Serani, Carlo Antonio Nuzzolo   +9 more
doaj   +1 more source

Deuterium Depletion Inhibits Cell Proliferation, RNA and Nuclear Membrane Turnover to Enhance Survival in Pancreatic Cancer

Cancer Control, 2021
The effects of deuterium-depleted water (DDW) containing deuterium (D) at a concentration of 25 parts per million (ppm), 50 ppm, 105 ppm and the control at 150 ppm were monitored in MIA-PaCa-2 pancreatic cancer cells by the real-time cell impedance ...
László G. Boros, Ildikó Somlyai, Beáta Zs. Kovács, László G. Puskás, Lajos I. Nagy, László Dux, Gyula Farkas, Gábor Somlyai   +7 more
doaj   +1 more source

Selbstassemblierte (Aza‐)BODIPY Farbstoffe für Biomedizinische In Vivo Bildgebung

Angewandte Chemie, EarlyView.
Dieses Minireview klassifiziert (Aza‐)BODIPY‐Farbstoffe anhand ihrer molekularen Stapelungsmodi und Aggregattypen (H‐, oblique‐ oder J‐Typ) in wässrigen Medien und setzt diese Merkmale in Beziehung zu ihrer multimodalen Bildgebungsleistung. Durch die Verknüpfung von molekularem Design, Exzitonenkopplung und pfadabhängiger Assemblierung stellen wir ...
Antonia Albers, Max Masthoff, Gustavo Fernández   +2 more
wiley   +1 more source

L-Lactate Electrochemical Biosensor Based on an Integrated Supramolecular Architecture of Multiwalled Carbon Nanotubes Functionalized with Avidin and a Recombinant Biotinylated Lactate Oxidase

Biosensors
L-Lactate is an important bioanalyte in the food industry, biotechnology, and human healthcare. In this work, we report the development of a new L-lactate electrochemical biosensor based on the use of multiwalled carbon nanotubes non-covalently ...
Alejandro Tamborelli, Michael López Mujica, Marilla Amaranto, José Luis Barra, Gustavo Rivas, Agustina Godino, Pablo Dalmasso   +6 more
doaj   +1 more source

Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter

BioTechniques, 2004
Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide
Arthur Karlin, Chaojian Wang, Jing Li, Qiang Xu   +3 more
doaj   +1 more source

Self‐Assembled (Aza)BODIPY Dyes for Biomedical in Vivo Imaging

Angewandte Chemie International Edition, EarlyView.
This minireview classifies (aza)BODIPY dyes according to their molecular stacking modes and aggregate types (H‐, oblique‐, or J‐type) in aqueous media, correlating these features with their multimodal imaging performance. By linking molecular design, exciton coupling, and pathway‐dependent assembly, we provide a framework for engineering next ...
Antonia Albers, Max Masthoff, Gustavo Fernández   +2 more
wiley   +1 more source

ISOLATION AND PURIFICATION OF AVIDIN FROM EGG WHITE BY IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY

The Iraqi Journal of Veterinary Medicine, 1996
Lysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme in pure form by immobilized metal affinity chromatography column (1.5 x 10cm) loaded with copper ions ...
Shalan Alwan Al-Mashikhi
doaj   +1 more source

Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery

MethodsX, 2020
Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues.
Chenzhen Zhang, Tengfei He, Armin Vedadghavami, Ambika G. Bajpayee   +3 more
doaj   +1 more source