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Purification of Axonemal Dyneins and Dynein-Associated Components from Chlamydomonas
Methods in Cell Biology, 2009Axonemal dyneins are responsible for generating the force required to power ciliary and flagellar motility. These highly complex enzymes form the inner and outer arms associated with the outer doublet microtubules. They are built around one or more ~520kD heavy chains that exhibit motor activity and also include additional components that are required ...
Stephen M KING
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Axonemal dynein from Tetrahymena
Journal of Cell Science, 1991ABSTRACT Axonemal dynein from Tetrahymena cilia can be separated on a sucrose gradient into two fractions, at least one of which appears to be polymorphic. We have been using immuno-electron microscopy in order to try and locate the different types of dynein molecules within the axonemal structure.
E M, Crossley, S C, Hyman, C, Wells
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Glycylation regulates axonemal dyneins
Science, 2021Cell Biology Physiological functions of the microtubule cytoskeleton are expected to be regulated by a variety of posttranslational tubulin modifications. For instance, tubulin glycylation is almost exclusively found in cilia and flagella, but its role in the function of these organelles remains unclear. Gadadhar et al.
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Biophysical Measurements on Axonemal Dyneins
2009In recent decades, the development of technologies such as optical trap nanometry and advanced fluorescence microscopy have provided tools for studying the dynamics of single protein molecules in vitro and in vivo with nanometer precision over timescales from milliseconds to seconds.
Hiroaki, Kojima +3 more
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Cytoskeleton, 2014
The Ephemeroptera sperm axoneme is devoid of outer dynein arms (ODA) and exhibits a pronounced modification of the central pair complex (CPC), which is substituted by the central sheath (CS): a tubular element of unknown molecular composition. We performed a detailed ultrastructural analysis of sperm axonemes in the genera Cloeon and Ecdyonurus using ...
MENCARELLI, CATERINA +3 more
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The Ephemeroptera sperm axoneme is devoid of outer dynein arms (ODA) and exhibits a pronounced modification of the central pair complex (CPC), which is substituted by the central sheath (CS): a tubular element of unknown molecular composition. We performed a detailed ultrastructural analysis of sperm axonemes in the genera Cloeon and Ecdyonurus using ...
MENCARELLI, CATERINA +3 more
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Cytoplasmic preassembly and trafficking of axonemal dyneins
2018David R Mitchell
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Axonemal dyneins winch the cilium
Nature Structural & Molecular Biology, 2010In this issue, Ishikawa and colleagues provide direct evidence to support the 'winch' hypothesis for the dynein motor mechanism, in which the AAA domain motor unit is displaced parallel to the doublet microtubule long axis rather than undergoing a rotary motion.
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