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Purification of Axonemal Dyneins and Dynein-Associated Components from Chlamydomonas

Methods in Cell Biology, 2009
Axonemal dyneins are responsible for generating the force required to power ciliary and flagellar motility. These highly complex enzymes form the inner and outer arms associated with the outer doublet microtubules. They are built around one or more ~520kD heavy chains that exhibit motor activity and also include additional components that are required ...
Stephen M KING
exaly   +3 more sources

Axonemal dynein from Tetrahymena

Journal of Cell Science, 1991
ABSTRACT Axonemal dynein from Tetrahymena cilia can be separated on a sucrose gradient into two fractions, at least one of which appears to be polymorphic. We have been using immuno-electron microscopy in order to try and locate the different types of dynein molecules within the axonemal structure.
E M, Crossley, S C, Hyman, C, Wells
openaire   +2 more sources

Glycylation regulates axonemal dyneins

Science, 2021
Cell Biology Physiological functions of the microtubule cytoskeleton are expected to be regulated by a variety of posttranslational tubulin modifications. For instance, tubulin glycylation is almost exclusively found in cilia and flagella, but its role in the function of these organelles remains unclear. Gadadhar et al.
openaire   +1 more source

Biophysical Measurements on Axonemal Dyneins

2009
In recent decades, the development of technologies such as optical trap nanometry and advanced fluorescence microscopy have provided tools for studying the dynamics of single protein molecules in vitro and in vivo with nanometer precision over timescales from milliseconds to seconds.
Hiroaki, Kojima   +3 more
openaire   +2 more sources

Ultrastructure of the sperm axoneme and molecular analysis of axonemal dynein in ephemeroptera (Insecta)

Cytoskeleton, 2014
The Ephemeroptera sperm axoneme is devoid of outer dynein arms (ODA) and exhibits a pronounced modification of the central pair complex (CPC), which is substituted by the central sheath (CS): a tubular element of unknown molecular composition. We performed a detailed ultrastructural analysis of sperm axonemes in the genera Cloeon and Ecdyonurus using ...
MENCARELLI, CATERINA   +3 more
openaire   +4 more sources

Axonemal dyneins winch the cilium

Nature Structural & Molecular Biology, 2010
In this issue, Ishikawa and colleagues provide direct evidence to support the 'winch' hypothesis for the dynein motor mechanism, in which the AAA domain motor unit is displaced parallel to the doublet microtubule long axis rather than undergoing a rotary motion.
openaire   +2 more sources

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