Results 101 to 110 of about 515,050 (293)

CRISPR base editors: genome editing without double-stranded breaks

, 2018
The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 adaptive immunity system has been harnessed for genome editing applications across eukaryotic species, but major drawbacks, such as the inefficiency of precise base editing and ...
Magdy M. Mahfouz, Ayman Eid, Sahar Alshareef   +2 more
core   +1 more source

An improved SNAP-ADAR tool enables efficient RNA base editing to interfere with post-translational protein modification

Nature Communications
RNA base editing relies on the introduction of adenosine-to-inosine changes into target RNAs in a highly programmable manner in order to repair disease-causing mutations.
Karthika Devi Kiran Kumar, Shubhangi Singh, Stella Maria Schmelzle, Paul Vogel, Carolin Fruhner, Alfred Hanswillemenke, Adrian Brun, Jacqueline Wettengel, Yvonne Füll, Lukas Funk, Valentin Mast, J. Josephine Botsch, Philipp Reautschnig, Jin Billy Li, Thorsten Stafforst   +14 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Therapeutic Base Editing of Human Hematopoietic Stem Cells

, 2022
Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to permanently remedy blood disorders, although its application in engrafting hematopoietic stem cells (HSCs) remains unexplored.
Zeng, Jing, Pinello, Luca, Gehrke, Jason M., Yao, Qiuming, Luk, Kevin, Wu, Yuxuan, Bonanno, Jasmine, Bauer, Daniel, Manis, John, Shen, Anne H., Ren, Chunyan, Kim, Rachel, Joung, Keith, Shea, Devlin, Wolfe, Scot A., Clement, Kendell   +15 more
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Efficient Generation of Pathogenic A-to-G Mutations in Human Tripronuclear Embryos via ABE-Mediated Base Editing

Molecular Therapy: Nucleic Acids, 2019
Base editing systems show their power in modeling and correcting the pathogenic mutations of genetic diseases. Previous studies have already demonstrated the editing efficiency of BE3-mediated C-to-T conversion in human embryos.
Guanglei Li, Xinyi Liu, Shisheng Huang, Yanting Zeng, Guang Yang, Zongyang Lu, Yu Zhang, Xu Ma, Lisheng Wang, Xingxu Huang, Jianqiao Liu   +10 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Predicting adenine base editing efficiencies in different cellular contexts by deep learning

Genome Biology
Background Adenine base editors (ABEs) enable the conversion of A•T to G•C base pairs. Since the sequence of the target locus influences base editing efficiency, efforts have been made to develop computational models that can predict base editing ...
Lucas Kissling, Amina Mollaysa, Sharan Janjuha, Nicolas Mathis, Kim F. Marquart, Yanik Weber, Woohyun J. Moon, Paulo J. C. Lin, Steven H. Y. Fan, Hiromi Muramatsu, Máté Vadovics, Ahmed Allam, Norbert Pardi, Ying K. Tam, Michael Krauthammer, Gerald Schwank   +15 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source