Results 201 to 210 of about 510,395 (260)

Noncanonical target-strand cytosine base editing via engineered Un1Cas12f1 platform. [PDF]

Nat Commun
Song Z, Guo J, Fan Z, Huang S, Li G, Zhao Z, Chen B, Huang S, Zheng W, Wei Y, Chen Y, Huang X, Liu J, Wu L, Wang X.   +14 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Coiled-coil heterodimer-mediated split base editing systems enable flexible and robust nucleotide substitutions. [PDF]

Nat Commun
Mu S, Li Q, Chen M, Li Z, Ma Y, Li Y, Song Y, Hou S, Ding Y, Ju J, Lin Y, Zhang J, Yang Y, Ren X, Li N, Jin Q, Lai L, Wang K, Shi H.   +18 more
europepmc   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs. [PDF]

Nat Chem Biol
Smargon AA, Pant D, Gomberg TA, Fagre C, Glynne S, Nguyen J, Naritomi JT, Gilbert WV, Yeo GW.   +8 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Correction: Targeted C-to-T Base Editing in the Arabidopsis Plastid Genome. [PDF]

Curr Protoc
Nakazato I, Arimura SI.
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Mini-Catalytically Inactive Cas13X-Derived RNA Base Editing of β-Catenin Attenuates Pulmonary Damage in a Murine Acute Lung Injury Model. [PDF]

MedComm (2020)
Liu W, Bi W, Hou S, Du J, Zeng L, Zhang A, Zhang H, Wen D, Cai Q, Gao C, Lin P, Wu M, Li L, Jiang J.   +13 more
europepmc   +1 more source

In vivo mitochondrial base editing restores genotype and visual function in a mouse model of LHON. [PDF]

Nat Commun
Kim S, Kim J, Cha S, Ju S, Lim CJ, Hong S, Bae J, Oh Y, Jung S, Kim SP, Shin HS, Yoon JH, Park J, Ryou S, Lim SY, Lee SB, Choi SH, Park SJ, Choi CG, Choi M, Kim LK, Park J, Lee S, Seo KY, Seong JK, Kim K, Kim JS, Lee H.   +27 more
europepmc   +1 more source