Results 221 to 230 of about 515,050 (293)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Therapeutic base editing to generate a gain-of-function F9 variant for hemophilia B.

Blood
Baatartsogt N, Kashiwakura Y, Hiramoto T, Ito R, Sato R, Nagao Y, Naruoka H, Takata H, Hayakawa M, Batjargal K, Togashi T, Hoshino A, Shimizu T, Sato Y, Ishida T, Nureki O, Ohmori T.   +16 more
europepmc   +1 more source

Cas9-Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A-To-G Base Editing in Rice. [PDF]

Plant Biotechnol J
Hu J, Li X, Gao Y, Guo Y, Liu Y, Wang C, Xu G, Du C, Liu S, Zhao Z, Wang Y, Wu Y, Dong X, Li C, Wan J.   +14 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Functional correction and genome integrity with duplex base editing of β-thalassemic hematopoietic stem cells. [PDF]

Genome Biol
Papaioannou NY, Patsali P, Klermund J, Papasavva PL, Andrieux G, Koniali L, Naiisseh B, Christou S, Sitarou M, Kleanthous M, Cathomen T, Lederer CW.   +11 more
europepmc   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Base Editing-Mediated Targeted Evolution of Populus CesA8 Boosts Cellulose Production. [PDF]

Plant Biotechnol J
Jia X, Zhang M, Zhu P, Zhong Y, Gui J.
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

CAR-adapted PIK3CD base editing enhances T cell anti-tumor potency. [PDF]

Nat Cancer
Bucher P, Brückner N, Kortendieck J, Grimm M, Schleicher JT, Bartels K, Hardy S, Rausch M, Wurzer H, Thiemann M, May C, Mitstorfer M, Letzgus D, Quach J, Schneider C, Ispan DA, Gonzalez-Menendez I, Jain N, Ho YJ, Chen J, Sánchez-Rivera FJ, Sun J, Quintanilla-Martinez L, Trautwein C, Weigelin B, Claassen M, Sadelain M, Feucht J, Leibold J.   +28 more
europepmc   +1 more source