Results 71 to 80 of about 4,580,472 (190)
Effective in vivo RNA base editing via engineered cytidine deaminase APOBECs fused with PUF proteins
Nature CommunicationsBase editing stands at the forefront of genetic engineering, heralding precise genetic modifications with broad implications. While CRISPR-based DNA and RNA base editing systems capitalize on sgRNA-guided specificity and diverse deaminase functionalities,
Wenjian Han +10 more
doaj +1 more source
Seamlessly Editing the Web [PDF]
, 2010 The typical process of editing content on the web is strongly moded. Authors are forced to switch between editing and previewing and publishing modes before, during, and after the editing process. This thesis explores a new paradigm of editing content on
Novak, Brook Jesse
core
Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
, 2023 openLa distrofia muscolare di Duchenne (DMD) è una malattia genetica rara e mortale, causata dalla mancanza di distrofina. Le mutazioni nel gene DMD, localizzato nel cromosoma X, sono trasmesse in modo recessivo legato al sesso; in particolare, una ...
DE ANTONI, NOEMI
core
Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
doaj +1 more source
In the business of base editors: Evolution from bench to bedside
PLoS Biology, 2023 With the advent of recombinant DNA technology in the 1970s, the idea of using gene therapies to treat human genetic diseases captured the interest and imagination of scientists around the world.
Elizabeth M. Porto, Alexis C. Komor
doaj
DNA and RNA base editors can correct the majority of pathogenic single nucleotide variants
npj Genomic MedicineThe majority of human genetic diseases are caused by single nucleotide variants (SNVs) in the genome sequence. Excitingly, new genomic techniques known as base editing have opened efficient pathways to correct erroneous nucleotides.
Ariel Dadush +8 more
doaj +1 more source
, 2019 Adenosine deaminases acting on RNA (ADARs) catalyze the deamination of adenosine (A) to inosine (I). A-to-I RNA editing targets double-stranded RNA (dsRNA), and increases the complexity of gene regulation by modulating base pairing-dependent processes ...
Rintaro Ishii (5462111) +7 more
core +1 more source
Nature CommunicationsRNA base editing relies on the introduction of adenosine-to-inosine changes into target RNAs in a highly programmable manner in order to repair disease-causing mutations.
Karthika Devi Kiran Kumar +14 more
doaj +1 more source
An optimized toolkit for precision base editing
, 2018 CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single base pair resolution. By re-engineering both DNA and protein sequences, we developed a collection of constitutive and inducible base ...
Lukas E Dow +16 more
core +1 more source
Predicting adenine base editing efficiencies in different cellular contexts by deep learning
Genome BiologyBackground Adenine base editors (ABEs) enable the conversion of A•T to G•C base pairs. Since the sequence of the target locus influences base editing efficiency, efforts have been made to develop computational models that can predict base editing ...
Lucas Kissling +15 more
doaj +1 more source