Results 121 to 130 of about 7,909 (199)

Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti

Scientific Reports, 2017
The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with ...
Najat Dzaki, Karima N. Ramli, Azali Azlan, I. Ishak, G. Azzam   +4 more
semanticscholar   +1 more source

Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae) [PDF]

, 2016
Citation: Barandoc-Alviar, K., Ramirez, G. M., Rotenberg, D., & Whitfield, A. E. (2016). Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae).
Barandoc-Alviar, K., Ramirez, G. M., Rotenberg, Dorith, Whitfield, Anna E.   +3 more
core   +1 more source

Identification of miR-16 as an endogenous reference gene for the normalization of urinary exosomal miRNA expression data from CKD patients

PLoS ONE, 2017
Chronic kidney disease (CKD) is a severe disorder with an increasing incidence worldwide. An early detection may help to prevent its progression and to minimize the risk of cardiovascular diseases as one of the major comorbidities.
Tim Lange, S. Stracke, R. Rettig, U. Lendeckel, Jana Kuhn, R. Schlüter, V. Rippe, K. Endlich, N. Endlich   +8 more
semanticscholar   +1 more source

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

International Journal of Molecular Sciences, 2018
Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used.
Yuning Hu, H. Fu, H. Qiao, Shengming Sun, Wenyi Zhang, S. Jin, Sufei Jiang, Y. Gong, Y. Xiong, Yan Wu   +9 more
semanticscholar   +1 more source

Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

BMC Research Notes, 2009
Background Reference genes are used as internal standards to normalize mRNA abundance in quantitative real-time PCR and thereby allow a direct comparison between samples.
Sommer Simone, Axtner Jan
doaj   +1 more source

Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine

Scientific Reports, 2018
Grapevine is among the fruit crops with high economic value, and because of the economic losses caused by abiotic stresses, the stress resistance of Vitis vinifera has become an increasingly important research area.
Meng Luo, Zhen Gao, Hui Li, Qin Li, Caixi Zhang, Wen-ping Xu, Shiren Song, Chao Ma, Shiping Wang   +8 more
semanticscholar   +1 more source

Evaluation of three reference genes of Escherichia coli for mRNA expression level normalization in view of salt and organic acid stress exposure in food [PDF]

, 2017
Escherichia coli can adapt to various stress conditions encountered in food through induction of stress response genes encoding proteins that counteract the respective stresses.
Hummerjohann, Jörg, Peng, Silvio, Stephan, Roger, Tasara, Taurai   +3 more
core  

Seleção de genes endógenos para análise de qRT-PCR em sementes de café. [PDF]

, 2014
A técnica quantitativa de PCR em tempo real, qRT-PCR, permite analisar a expressão de genes alvo em diferentes condições experimentais. No entanto, a análise precisa dos resultados gerados pela qRT-PCR está diretamente relacionada ao gene normalizador ...
CARDOSO, D. C., MALUF, M. P., PADILHA, L., SCHENK, J. C. M.   +3 more
core  

Expression stability of commonly used reference genes in canine articular connective tissues [PDF]

, 2007
Background The quantification of gene expression in tissue samples requires the use of reference genes to normalise transcript numbers between different samples.
Ayers, Duncan, Clements, Dylan N., Day, Philip, Day, Philip J R, Marriage, Fiona, Salway, Fiona   +5 more
core   +3 more sources

Selection of stable reference genes in Prunus persica fruit infected with Monilinia laxa for normalisation of RT-qPCR gene expression data

Scientific Reports
Reverse transcription-quantitative PCR (RT-qPCR) is a powerful tool for quantifying gene expression. However, reference genes (RGs) for gene expression analysis in peach (Prunus persica) during interactions with Monilinia laxa, a major fungal pathogen ...
Agata Lizzio, Valerio Battaglia, Ernesto Lahoz, Massimo Reverberi, Milena Petriccione   +4 more
doaj   +1 more source