Results 81 to 90 of about 7,909 (199)

Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera

Molecular Ecology, Volume 34, Issue 15, August 2025.
Abstract Quantitative real‐time polymerase chain reaction (qPCR) is a method widely used to determine changes and differences in gene expression. As target gene expression is most often quantified relative to the expression of reference genes, the validation of suitable reference genes is of critical importance.
Anja Buttstedt, Christian W. W. Pirk, Abdullahi A. Yusuf   +2 more
wiley   +1 more source

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes

Current Issues in Molecular Biology, 2021
Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez, Leticia Melgar-Vilaplana, Elizabeth Córdoba-Lanús, Ricardo Fernández-de-Misa   +3 more
doaj   +1 more source

Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta)

BMC Plant Biology
Taro is a widely utilized starch resource plant. It is essential to quantify the expression levels of functional genes associated with taro growth using real-time quantitative polymerase chain reaction (RT-qPCR).
Weiqing Dong, Qi Chen, Fanglian He
semanticscholar   +1 more source

Highly Targeted Metabolomics Coupled With Gene Expression Analysis by RT–qPCR Improves Beef Separation Based on Grass, Grain, or Grape Supplemented Diet

Food Frontiers, Volume 6, Issue 3, Page 1483-1497, May 2025.
ABSTRACT The objective of this study was to use a multi‐omics (i.e., gene expression quantification, metabolomics, and fatty acid [FA] profiling) approach to separate and authenticate beef from three different dietary groups. In this 2‐year study, Red Angus steers (n = 54) were randomly allocated to one of three treatments: (1) complex biodiverse ...
Lucas Krusinski, Chloe Castanon, Rosalee S. Hellberg, Isabella C. F. Maciel, Muhammad Ahsin, Stephan van Vliet, Jason E. Rowntree, Jenifer I. Fenton   +7 more
wiley   +1 more source

Study of Some Reference Genes Expression Stability in Rice Using Real-Time PCR Method Under Biotic Stress [PDF]

مجله بیوتکنولوژی کشاورزی, 2019
Objective Gene expression studies by Real-Time PCR constitute a powerful tool to analyze the mechanisms underlying plant biotic-stress tolerance. One of the crucial steps of this technique is the selection and validation of reference genes to normalize ...
Hamidreza Ghorbani, Seyyed Hamidreza Hashemi-petroudi, Mehdi Rostami Rostami   +2 more
doaj   +1 more source

Determination of internal controls for quantitative gene expression of Spodoptera litura under microbial pesticide stress

Scientific Reports
Quantitative real-time polymerase chain reaction (qRT-PCR) has become a commonly used method for the quantification of gene expression. However, accurate qRT-PCR analysis requires a valid internal reference for data normalization.
Shuang Wu, Yunmi Luo, Zhihong Zeng, Ying Yu, Shicai Zhang, Yan Hu, Lei Chen   +6 more
semanticscholar   +1 more source

Validation of reference genes for normalization of qPCR mRNA expression levels in Staphylococcus aureus exposed to osmotic and lactic acid stress conditions encountered during food production and preservation [PDF]

, 2017
Staphylococcus aureus represents the most prevalent cause of food-borne intoxications worldwide. While being repressed by competing bacteria in most matrices, this pathogen exhibits crucial competitive advantages during growth at high salt concentrations
Johler, Sophia, Sihto, Henna-Maria, Stephan, Roger, Tasara, Taurai   +3 more
core  

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source

Pathogen and Gene Expression Profiles of Atlantic Salmon From an Endangered Population

Aquatic Conservation: Marine and Freshwater Ecosystems, Volume 35, Issue 4, April 2025.
ABSTRACT The role of pathogens in impacting the behaviour and fate of salmonids has been studied extensively for some selected pathogens such as sea lice. However, the whole pathobiome of the fish are seldom considered and may confound and influence the study species in situ.
Robert J. Lennox, Angela D. Schulze, Kristi M. Miller, Trond Einar Isaksen, Eirik Straume Normann, Cecilie Iden Nilsen, Lotte S. Dahlmo, Bjørn T. Barlaup, Elisabeth Stöger, Sindre H. Eldøy, Knut Wiik Vollset   +10 more
wiley   +1 more source

Selection and Validation of Reference Genes for Gene Expression Studies in Euonymus japonicus Based on RNA Sequencing

Genes
Euonymus japonicus is one of the most low-temperature-tolerant evergreen broad-leaved tree species in the world and is widely used in urban greening. However, there are very few molecular biology studies on its low-temperature tolerance mechanism. So far,
Wei Guo, Yihui Yang, Bo Ma, Wenbo Wang, Zenghui Hu, Ping-Sheng Leng   +5 more
semanticscholar   +1 more source