Results 81 to 90 of about 6,321 (156)

Selection and Validation of Appropriate Reference Genes for qRT‐PCR Analysis of Iris germanica L. Under Various Abiotic Stresses

Food Science &Nutrition, Volume 13, Issue 1, January 2025.
In this research, we evaluated the expression stability of 9 candidate reference genes by four different algorithms (GeNorm, Normfinder, BestKeeper, and RefFinder). ACT and F3H appeared to be the most reliable reference genes under different abiotic stresses.
Yuan Yuan, Chungui Liu, Jianzhong Bao, Fengtong Li   +3 more
wiley   +1 more source

Plasma miR‐1‐3p levels predict severity in hospitalized COVID‐19 patients

British Journal of Pharmacology, Volume 182, Issue 2, Page 451-467, January 2025.
Background and Purpose Accumulating evidence suggests circulating microRNAs (miRNAs) are important regulators of biological processes involved in COVID‐19 complications. We sought to assess whether circulating miRNAs are associated with COVID‐19 clinical phenotype and outcome.
Paola Di Pietro, Angela Carmelita Abate, Carmine Izzo, Anna Laura Toni, Maria Rosaria Rusciano, Veronica Folliero, Federica Dell'Annunziata, Giovanni Granata, Valeria Visco, Benedetta Maria Motta, Alfonso Campanile, Carolina Vitale, Valeria Prete, Cristina Gatto, Giuliana Scarpati, Paolo Poggio, Gennaro Galasso, Pasquale Pagliano, Ornella Piazza, Gaetano Santulli, Gianluigi Franci, Albino Carrizzo, Carmine Vecchione, Michele Ciccarelli   +23 more
wiley   +1 more source

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source

Insulin and leptin acutely modulate the energy metabolism of primary hypothalamic and cortical astrocytes

Journal of Neurochemistry, Volume 169, Issue 1, January 2025.
Astrocytes are a heterogeneous population of brain cells which adapt their properties to the requirements of the local neural circuitry. Using metabolic nanosensors, this study shows distinct differences between the metabolic state of astrocytes from the cortex and hypothalamus under basal conditions and after stimulation with insulin and leptin ...
Christopher Wolff, Dorit John, Ulrike Winkler, Luise Hochmuth, Johannes Hirrlinger, Susanne Köhler   +5 more
wiley   +1 more source

Identification of Suitable Reference Genes for Gene Expression Normalization in Jatropha curcas L During Development and Under Stress Conditions Using Real Time Quantitative PCR

Brazilian Archives of Biology and Technology
Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues.
Antonio José Rocha, Paulo Abraão Maranhão, Rafaela Oliveira Silva, Simone Pohl, Cristiane S.R Fonteles   +4 more
doaj   +1 more source

Cloning, Expression and Stability Analysis of Candidate Reference Genes in the Glacial Relict Hucho bleekeri Under Immune Stress

Journal of Applied Ichthyology, Volume 2025, Issue 1, 2025.
To find reference genes (RGs) and establish a screening method for RGs in Sichuan taimen (Hucho bleekeri), six candidate RGs—β-actin, b2m, β-tubulin, ef1α, gapdh and hprt — were cloned, and the stability of expression of the six candidate RGs were analysed. The six RG sequences in H.
Xiaoyun Wu, Jiansheng Lai, Yeyu Chen, Huanchao Yang, Yanling Chen, Zhao Liu, Quanyu Tu, Yi Yu, Hua Li, Poulami Jha   +9 more
wiley   +1 more source

NormiRazor: tool applying GPU-accelerated computing for determination of internal references in microRNA transcription studies

BMC Bioinformatics, 2020
Background Multi-gene expression assays are an attractive tool in revealing complex regulatory mechanisms in living organisms. Normalization is an indispensable step of data analysis in all those studies, since it removes unwanted, non-biological ...
Szymon Grabia, Urszula Smyczynska, Konrad Pagacz, Wojciech Fendler   +3 more
doaj   +1 more source

Selection of reference genes for expression studies with fish myogenic cell cultures [PDF]

, 2009
Indi
Ian A Johnston, Neil I Bower
core   +3 more sources

Duplicated membrane estrogen receptors in the European sea bass (Dicentrarchus labrax): Phylogeny, expression and regulation throughout the reproductive cycle [PDF]

, 2018
The numerous estrogen functions reported across vertebrates have been classically explained by their binding to specific transcription factors, the nuclear estrogen receptors (ERs).
Alvarado, M. Victoria, Andrade, André, Estêvão, M. Dulce, Felip, Alicia, Pinto, Patricia IS, Power, Deborah   +5 more
core   +1 more source

Selection and validation of reference genes desirable for gene expression analysis by qRT-PCR in MeJA-treated ginseng hairy roots.

PLoS ONE, 2019
Ginseng is a valuable herb of traditional Chinese medicine and ginsenosides, the main bioactive components of ginseng, have been proven to have multiple functions in human therapies and health.
Li Li, Kangyu Wang, Mingzhu Zhao, Shaokun Li, Yue Jiang, Lei Zhu, Jing Chen, Yanfang Wang, Chunyu Sun, Ping Chen, Jun Lei, Meiping Zhang, Yi Wang   +12 more
doaj   +1 more source