Results 81 to 90 of about 6,430 (223)

Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.

Scientific Reports
The selection of stable reference genes for the normalization of reverse transcription quantitative real-time PCR (RT-qPCR) is generally overlooked despite being the crucial element in determining the accuracy of the relative expression of genes.
Saranya Vijayakumar, Manjula Sakuntala
semanticscholar   +1 more source

BestKeeper stability analysis.

, 2014
Evaluation of gene expression stability by BestKeeper for individual brain regions. The Pearson correlation coefficient (R) of all HKG candidates. All HKG except 18S in the temporal cortex, presented significant correlation (P0.05 HC, hippocampus; HT ...
Juliana Cini Perry (640841), Jair Ribeiro Chagas (640842), Guilherme Silva Julian (640839), Renato Watanabe de Oliveira (640840), Sergio Tufik (74924)   +4 more
core   +1 more source

Crataegus oxyacantha Extract Enhances Cardiac Adaptation and Reduces Oxidative‐Inflammatory Stress in Broiler Chickens Raised at High Altitude Under Cold Stress

Veterinary Medicine and Science, Volume 12, Issue 4, July 2026.
Crataegus oxyacantha extract at 0.4 mL/L improved cardiac function in broilers under cold stress and high altitude by increasing nitric oxide synthase gene expression, boosting nitric oxide, reducing endothelin‐1 gene expression, and lowering oxidative‐inflammatory stress, offering a natural solution to mitigate pulmonary hypertension syndrome ...
Behnam Ahmadipour, Hossein Hassanpour, Samira Abaszadeh, Majid Kalantar   +3 more
wiley   +1 more source

Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation in Camellia impressinervis

International Journal of Molecular Sciences
Real-time quantitative PCR (qRT-PCR) is a pivotal technique for gene expression analysis. To ensure reliable and accurate results, the internal reference genes must exhibit stable expression across varied experimental conditions.
Peilan Zhang, Shu-Ying Chen, Si-Yu Chen, Yuanming Zhu, Yuqing Lin, Xinyu Xu, Zhong-Jian Liu, Shuangquan Zou   +7 more
semanticscholar   +1 more source

Expression stability of candidate reference genes as determined by BestKeeper.

, 2022
Expression stability of candidate reference genes as determined by BestKeeper.
María Cristina Romero-Rodríguez (13205687), Belén Gaete Humada (13205681), Julio C. M. Iehisa (3947534), Aníbal Arévalos (13205675), Amiliana Pineda-Fretez (13205684), María Eugenia Flores Giubi (13205690), Man Mohan Kohli (12989922), Belén Ortíz (13205678), Adriana Orrego (13205669), María Cecilia Gavilán (13205672)   +9 more
core   +1 more source

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Frontiers in Physiology
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited ...
Chen-Hui Shen, Min Tang, Xiaofei Li, Li Zhu, Wei Li, Pan Deng, Qing Zhai, Gang Wu, Xiaohong Yan   +8 more
semanticscholar   +1 more source

Type I Interferon Gene Expression and Its Association With Ocular Involvement in Systemic Lupus Erythematosus

ACR Open Rheumatology, Volume 8, Issue 2, February 2026.
Objective Severalstudies have highlighted the role of Type I interferons (IFN‐I) in activating inflammatory pathways in lupus. However, no previous research focused on investigating the role of IFN‐I gene expression in the ophthalmologic involvement of systemic lupus erythematosus (SLE).
Raphael Teixeira Costa, Werbson Lima Guaraná, Braziliano Miguel da Silva Júnior, Jaciel Clementino, Luís Sousa Inês, Paula Sandrin‐Garcia, Thiago Sotero Fragoso   +6 more
wiley   +1 more source

Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta)

BMC Plant Biology
Taro is a widely utilized starch resource plant. It is essential to quantify the expression levels of functional genes associated with taro growth using real-time quantitative polymerase chain reaction (RT-qPCR).
Weiqing Dong, Qi Chen, Fanglian He
semanticscholar   +1 more source

Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR

Scientific Reports, 2018
For reliable results, Reverse Transcription Quantitative real-time Polymerase Chain Reaction (RT-qPCR) analyses depend on stably expressed reference genes for data normalization purposes.
A. E. I. Gomes, Leonardo P Stuchi, Nathália Maria Gonçalves Siqueira, João Henrique, R. Vicentini, M. Ribeiro, M. Darrieux, L. Ferraz   +7 more
semanticscholar   +1 more source

Rank of 19 CIRGs calculated by NormFinder and BestKeeper.

, 2016
Rank of 19 CIRGs calculated by NormFinder and BestKeeper.
Qiankun Zhu (755358), Yi Liang (110469), Nan Lv (798889), Wei Pei (390028), Jing Fan (149601), Wanjun Wang (755360), Hongqiang An (3332835), Yihui Cui (169693)   +7 more
core   +1 more source