Results 21 to 30 of about 100,345 (222)

A light-switching pyrene probe to detect phase-separated biomolecules

iScience, 2021
Summary: Biomolecules may undergo liquid–liquid phase separation (LLPS) to spatiotemporally compartmentalize and regulate diverse biological processes. Because the number of tools to directly probe LLPS is limited (ie. FRAP, FRET, fluorescence microscopy,
Masaharu Hazawa, Shogo Amemori, Yoshio Nishiyama, Yoshihiro Iga, Yuki Iwashima, Akiko Kobayashi, Hirohisa Nagatani, Motohiro Mizuno, Kenji Takahashi, Richard W. Wong   +9 more
doaj   +1 more source

Multiplexed protein profiling reveals spatial subcellular signaling networks

iScience, 2022
Summary: Protein-protein interaction networks are altered in multi-gene dysregulations in many disorders. Image-based protein multiplexing sheds light on signaling pathways to dissect cell-to-cell heterogeneity, previously masked by the bulk assays ...
Shuangyi Cai, Thomas Hu, Mythreye Venkatesan, Mayar Allam, Frank Schneider, Suresh S. Ramalingam, Shi-Yong Sun, Ahmet F. Coskun   +7 more
doaj   +1 more source

SkewC: Identifying cells with skewed gene body coverage in single-cell RNA sequencing data

iScience, 2022
Summary: The analysis and interpretation of single-cell RNA sequencing (scRNA-seq) experiments are compromised by the presence of poor-quality cells. For meaningful analyses, such poor-quality cells should be excluded as they introduce noise in the data.
Imad Abugessaisa, Akira Hasegawa, Shuhei Noguchi, Melissa Cardon, Kazuhide Watanabe, Masataka Takahashi, Harukazu Suzuki, Shintaro Katayama, Juha Kere, Takeya Kasukawa   +9 more
doaj   +1 more source

Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age

iScience, 2023
Summary: Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (12 m.o.).
Collyn M. Kellogg, Kevin Pham, Sunghwan Ko, Jillian E.J. Cox, Adeline H. Machalinski, Michael B. Stout, Amanda L. Sharpe, Michael J. Beckstead, Ana J. Chucair-Elliott, Sarah R. Ocañas, Willard M. Freeman   +10 more
doaj   +1 more source

Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids

iScience, 2022
Summary: Cell-free DNA (cfDNA) has become the predominant analyte of liquid biopsy; however, recent studies suggest the presence of subnucleosomal-sized DNA fragments in circulation that are likely single-stranded.
Lauren Y. Cheng, Peng Dai, Lucia R. Wu, Abhijit A. Patel, David Yu Zhang   +4 more
doaj   +1 more source

Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy

iScience, 2021
Summary: Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of complex, dynamic, and heterogeneous biological systems.
Jingwen Shou, Robert Oda, Fanghao Hu, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Bruce Shiramizu, Wei Min, Yasuyuki Ozeki   +8 more
doaj   +1 more source

3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast

iScience, 2022
Summary: The placental syncytiotrophoblast, a syncytium without cell-cell junctions, is the primary barrier between the mother and the fetus. Despite no apparent anatomical pathway for paracellular diffusion of solutes across the syncytiotrophoblast ...
Rohan M. Lewis, Harikesan Baskaran, Jools Green, Stanimir Tashev, Eleni Palaiologou, Emma M. Lofthouse, Jane K. Cleal, Anton Page, David S. Chatelet, Patricia Goggin, Bram G. Sengers   +10 more
doaj   +1 more source

Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7”

iScience
Summary: Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and “OP7”, a new type of influenza ...
Daniel Rüdiger, Julita Piasecka, Jan Küchler, Carolina Pontes, Tanja Laske, Sascha Y. Kupke, Udo Reichl   +6 more
doaj   +1 more source

Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus

iScience, 2022
Summary: The choroid plexus (CP) is a highly vascularized structure containing endothelial and epithelial cells located in the ventricular system of the central nervous system (CNS).
Walter Muranyi, Christian Schwerk, Rosanna Herold, Carolin Stump-Guthier, Marko Lampe, Petra Fallier-Becker, Christel Weiß, Carsten Sticht, Hiroshi Ishikawa, Horst Schroten   +9 more
doaj   +1 more source