Results 1 to 10 of about 12,382 (217)

Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfection

Molecular Therapy: Methods & Clinical Development
Recombinant adeno-associated virus (rAAV)-based gene therapy is entering clinical and commercial stages at an unprecedented pace. Triple transfection of HEK293 cells is currently the most widely used platform for rAAV manufacturing.
Hao Liu, Yue Zhang, Mitchell Yip, Lingzhi Ren, Jialing Liang, Xiupeng Chen, Nan Liu, Ailing Du, Jiaming Wang, Hao Chang, Hyejin Oh, Chen Zhou, Ruxiao Xing, Mengyao Xu, Peiyi Guo, Dominic Gessler, Jun Xie, Phillip W.L. Tai, Guangping Gao, Dan Wang   +19 more
doaj   +1 more source

Advances and Challenges in Biomanufacturing of Glycosylation of Natural Products

Fermentation
Glycosylation is one of the most common and important modifications in natural products (NPs), which can alter the biological activities and properties of NPs, effectively increase structural diversity, and improve pharmacological activities.
Shunyang Hu, Bangxu Wang, Liang Pei, Jisheng Wang, Ya Gan, Liangzhen Jiang, Bingliang Liu, Jie Cheng, Wei Li   +8 more
doaj   +1 more source

Cascade Dielectrophoretic Separation for Selective Enrichment of Polyhydroxybutyrate (PHB)-Producing Cyanobacterium Synechocystis sp. PCC 6803

Micromachines
Maintaining favorable biological productivities in photosynthetic biomanufacturing systems, especially when the risk of contamination with competing microbes is high, remains a challenge to achieve while maintaining economic feasibility.
Songyuan Yan, Sara Louise Pacheco, Asa K. Laskie, Cesar Raul Gonzalez Esquer, Lawrence Kulinsky   +4 more
doaj   +1 more source

Challenges and Opportunities in Lentivirus Viral Vector Manufacturing for In Vivo Applications

Biomedicines
The clinical success of chimeric antigen receptor (CAR) T-cell therapies has revolutionized oncology, yet the high costs and logistical complexities of ex vivo manufacturing remain significant barriers to global patient access.
Eduardo Barbieri, Caryn L. Heldt
doaj   +1 more source

The application of tissue engineering in cartilage regeneration: technological advances and future challenges

Frontiers in Bioengineering and Biotechnology
Cartilage tissue, owing to its avascular, aneural, and alymphatic nature, possesses a highly limited capacity for self-repair. Damage caused by trauma, degenerative diseases, or congenital malformations seldom heals spontaneously, constituting a long ...
Hongyu Zhang, Jingwei Feng, Jiajun Zhi, Yiwen Deng, Ronghua Zou, Liulin Fu, Haiyue Jiang   +6 more
doaj   +1 more source

3D Bioprinting- An Advanced Manufacturing Process for Healthcare Applications

Innovation of Chemistry & Materials for Sustainability
3D bioprinting is a cutting-edge technique used to create intricate mechanical and biological structures. It was developed to impart few advanced features to the process of biomanufacturing mainly for healthcare applications.
Prosenjit Saha, Shrabani Bhunia, Gouripriya D. A, Anish Deb, Poonam Debnath, Sneha Banerjee, Subhajit Ghosh, Pooja Ghosh   +7 more
doaj   +1 more source

Natural polysaccharides as immune adjuvants to overcome immune checkpoint inhibitor resistance: mechanistic insights, clinical evidence, and translational challenges

Frontiers in Immunology
Immune checkpoint inhibitors (ICIs) have redefined the treatment landscape of advanced malignancies, yet primary and acquired resistance severely limits their therapeutic efficacy across most solid tumors.
Yuhua Li, Yuhua Li, Jiao Qian, Shurong Zhou, Chunyan Wang, De Cai   +5 more
doaj   +1 more source

A Self-Consistent, High-Fidelity Adsorption Model for Chromatographic Process Predictions: Low-to-High Load Density and Charge Variants in a Preparative Cation Exchanger

Separations
The development of ion exchange chromatography to polish biopharmaceuticals requires extensive experimental benchmarking. As part of the Design of Experiments (DoE), statistical models increased efficiency somewhat and are still state of the art; however,
Gregor M. Essert, Marko Tesanovic, Sonja Berensmeier, Isabell Hagemann, Peter Schwan   +4 more
doaj   +1 more source

Enzyme Catalysis for Sustainable Value Creation Using Renewable Biobased Resources

Molecules
Enzyme catalysis was traditionally used by various human cultures to create value long before its basic concepts were uncovered. This was achieved by transforming the raw materials available from natural resources into useful products.
Roland Wohlgemuth
doaj   +1 more source

A unifying equation for fermentation sustainability across the titer-rate-yield landscape. [PDF]

Nat Commun
Bhagwat SS, Rao CV, Zhao H, Singh V, Guest JS.   +4 more
europepmc   +1 more source