Results 131 to 140 of about 3,391 (172)
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BIOPAN-flight experiment "card"
IEEE Transactions on Nuclear Science, 1994As part of the BIOPAN-O test flight payload, ESA/ESTEC together with University of Siegen, designed, an experiment called CARD, for flight on the first BIOPAN model. The CARD experiment, consisting of commercially available 128 K-bit EEPROM cards and CR-39 plastic nuclear track detector foils, was flown in order to assess the EEPROMs sensitivity to ...
R. Harboe-Sorensen +6 more
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Biopanning for the Characterization of Allergen Mimotopes
2008Proper understanding of the pathogenesis of type I allergy relies on the identification of allergen epitopes. The phage display technique is a relatively new one to define peptide structures that mimic natural epitopes, including conformational B-cell epitopes.
Isabella, Pali-Schöll +1 more
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Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands [PDF]
Methods in Molecular Biology, 2009 One limitation in the development of biosensors for the early detection of disease is the availability of high specificity and affinity ligands for biomarkers that are indicative of a pathogenic process. Within the past 10 years, biopanning of phage displayed peptide libraries on intact cells has proven to be a successful route to the identification of
Kathlynn C Brown +2 more
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Biopanning and rapid analysis of selective interactive ligands
Nature Medicine, 2001Here we introduce a new approach for the screening, selection and sorting of cell-surface-binding peptides from phage libraries. Biopanning and rapid analysis of selective interactive ligands (termed BRASIL) is based on differential centrifugation in which a cell suspension incubated with phage in an aqueous upper phase is centrifuged through a non ...
R J, Giordano +4 more
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Esa's biopan 1 — “Vitamin” experiment preliminary results
Acta Astronautica, 1995The purpose of "Vitamin" experiment is to study the efficiency of protective substances on three biological acellular systems aqueous solutions exposed to cosmic radiation in space. The first system "LDL" is a low density lipoprotein. The second is "E2-TeBG complexe" in which estradiol (E2) is bound to its plasmatic carrier protein, testosterone ...
J P, Moatti +6 more
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Auto-Panning: a highly integrated and automated biopanning platform for peptide screening
Lab on a Chip, 2021Based on digital microfluidics, a highly-automated and integrated phage display platform (Auto-Panning) was developed for peptide screening.
Junxia Wang +9 more
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Monitoring Phage Biopanning by Next-Generation Sequencing
2017Phage display has enabled the rapid isolation of antigen-specific antibodies from combinatorial libraries of the variable heavy chain gene (VH) and variable light chain gene (VL). The method is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection by phage biopanning.Next-Generation Sequencing (NGS) coupled with
Anna, Vaisman-Mentesh, Yariv, Wine
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Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN
Biochemical and Biophysical Research Communications, 2021In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization.
Yibing, Wang +4 more
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Chapter Seven Biopanning by activation tagging
2002Publisher Summary This chapter focuses on biopanning by activation tagging. Extensive gene duplication in plants and the potential of functional redundancy among homologous sequences represent a hurdle in understanding physiological and biochemical functions of a vast number of plant genes by using conventional loss-of-function mutant screen ...
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Bacteria-based in vivo peptide library screening using biopanning approach
The Journal of Microbiology, 2011Traditionally, library screening has been performed to identify biologically active agents including small molecules or peptides that inhibit target proteins or molecules with therapeutic interests. Due to its chemical nature, library screening is usually performed under in vitro environments using purified proteins and molecules.
Ji-Hyeon, Choi, Sang-Hyun, Park
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