Results 141 to 150 of about 71,391 (320)

Flux Sampling Suggests Metabolic Signatures of High Antibody‐Producing CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Chinese hamster ovary (CHO) cells remain the industry standard for producing numerous therapeutic proteins, particularly monoclonal antibodies (mAbs). However, achieving higher recombinant protein titers remains an ongoing challenge and a fundamental understanding of the cellular mechanism driving improved bioprocess performance remains ...
Kate E. Meeson, Joanne Watson, Susan Rosser, Ellie Hawke, Andrew Pitt, Tessa Moses, Leon Pybus, Magnus Rattray, Alan J. Dickson, Jean‐Marc Schwartz   +9 more
wiley   +1 more source

Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Acetobacterium woodii is known to produce mainly acetate from CO2 and H2, but the production of higher value chemicals is desired for the bioeconomy. Using chain‐elongating bacteria, synthetic co‐cultures have the potential to produce longer‐chained products such as caproic acid.
Jan Herzog, Alexander Mook, Lotta Guhl, Miriam Bäumler, Matthias H. Beck, Dirk Weuster‐Botz, Frank R. Bengelsdorf, An‐Ping Zeng   +7 more
wiley   +1 more source

Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, EarlyView.
The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale, Luisa Blöbaum, Alexander Grünberger, Lisbeth Olsson   +3 more
wiley   +1 more source

FAIR research data management as community approach in bioengineering

Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023
Abstract Research data management (RDM) requires standards, policies, and guidelines. Findable, accessible, interoperable, and reusable (FAIR) data management is critical for sustainable research. Therefore, collaborative approaches for managing FAIR‐structured data are becoming increasingly important for long‐term, sustainable RDM.
Martina Rehnert, Ralf Takors
wiley   +1 more source

Improvement of ribonucleic acid production in Cyberlindnera jadinii and optimization of fermentation medium

AMB Express
To enhance the ribonucleic acid (RNA) productivity for industrial applications, this study employed strain screening and medium optimization to improve the content of RNA in Cyberlindnera jadinii.
Mengting Li, Shuhong Gao, Pengcheng Yang, Hejin Li   +3 more
doaj   +1 more source

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source

A practical application of methodologies to determine asset criticality and work order prioritization

Engineering Reports, Volume 4, Issue 12, December 2022., 2022
The ability of a company to prioritise work orders is an essential part of managing work which in turn is vital when it comes to delivering maintenance services. The authors have drafted a tutorial paper that outlines a real‐world application of practices which determine asset criticality and work order prioritisation. Abstract The ability of a company
Lar English, Akilu Yunusa‐Kaltungo
wiley   +1 more source

A New Aerosol‐Based Photobioreactor for the Cultivation of Cyanobacteria on Luffa

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cyanobacteria are promising organisms for sustainable biotechnology due to their ability to grow photoautotrophically and their wide range of products. Many cyanobacteria grow in the form of biofilms, which is why the development of photobioreactors (PBR) for the cultivation of cyanobacteria in the form of biofilms is of great interest ...
Jonas Kollmen, Andreas Stiefelmaier, Ramtin Mofrad, Dorina Strieth   +3 more
wiley   +1 more source

Exploration of agricultural residue ash as a solid green heterogeneous base catalyst for biodiesel production

Engineering Reports, Volume 5, Issue 1, January 2023., 2023
Transesterification of vegetable oils with homogeneous catalyst for biodiesel synthesis is considered to have a promising future, but it is nonrenewable and hazardous. High production cost, in terms of feedstock oil and catalyst acquisition is one of the major challenges facing the global commercialization of biodiesel.
Christopher Tunji Oloyede, Simeon Olatayo Jekayinfa, Abass Olanrewaju Alade, Oyetola Ogunkunle, Nsikak‐Abasi Ubohon Otung, Opeyeolu Timothy Laseinde   +5 more
wiley   +1 more source

Temporal Galactose‐Manganese Feeding in Fed‐Batch and Perfusion Bioreactors Modulates UDP‐Galactose Pools for Enhanced mAb Glycosylation Homogeneity

Biotechnology and Bioengineering, EarlyView.
This study investigates the temporal effects of galactose and manganese on the N‐glycosylation of Trastuzumab (mAb) antibody during fed‐batch and perfusion bioprocessing modes to reveal the influence of feeding schedules on mAb N‐glycosylation patterns. These findings will aid in building process control schemes for mAb bioprocessing.
Aron Gyorgypal, Erica Fratz‐Berilla, Casey Kohnhorst, David N. Powers, Shishir P. S. Chundawat   +4 more
wiley   +1 more source