Brain organoid protocols and limitations [PDF]
Frontiers in Cellular NeuroscienceStem cell-derived organoid technology is a powerful tool that revolutionizes the field of biomedical research and extends the scope of our understanding of human biology and diseases.
Helen H. Zhao +3 more
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Deciphering brain organoid heterogeneity by identifying key quality determinants [PDF]
Communications BiologyBrain organoids derived from human pluripotent stem cells (hPSCs) hold immense potential for modeling neurodevelopmental processes and disorders. However, their experimental variability and undefined organoid selection criteria for analysis hinder ...
Tom Boerstler +19 more
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Japanese attitudes toward cell donation in human brain organoid research: many oppose broad consent [PDF]
Frontiers in GeneticsIntroductionUnder broad consent, donors are not informed about the specific research projects using their cells; this may lead to the use of cells in ways that conflict with donors' moral beliefs.
Masanori Kataoka +4 more
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Single-cell analysis reveals shared and distinct molecular signatures in brain organoid models of neurodegeneration and neuroinflammation [PDF]
Alzheimer’s Research & TherapyBackground Alzheimer’s disease (AD) and Parkinson’s disease (PD) are complex neurodegenerative disorders with common pathological features, but the molecular mechanisms underlying their early stages remain poorly understood.
Sophie Le Bars +4 more
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A large and diverse brain organoid dataset of 1,400 cross-laboratory images of 64 trackable brain organoids [PDF]
Scientific DataBrain organoids represent a useful tool for modeling of neurodevelopmental disorders and can recapitulate brain volume alterations such as microcephaly. To monitor organoid growth, brightfield microscopy images are frequently used and evaluated manually ...
Julian Schröter +6 more
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Neuroligin-3 R451C induces gain-of-function gene expression in astroglia in an astroglia-enriched brain organoid model [PDF]
Cell RegenerationAstroglia are integral to brain development and the emergence of neurodevelopmental disorders. However, studying the pathophysiology of human astroglia using brain organoid models has been hindered by inefficient astrogliogenesis.
Rui Dang +8 more
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A review of protocols for brain organoids and applications for disease modeling
STAR Protocols, 2023 Summary: Recent breakthroughs in human stem cell technologies have enabled the generation of 3D brain organoid platforms for modeling human neurodevelopment and disease.
Christopher N. Mayhew, Richa Singhania
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How well do brain organoids capture your brain?
iScience, 2021 Summary: Brain organoids closely recapitulate many features and characteristics of in vivo brain tissue. This technology in turn allows unprecedented possibilities to investigate brain development and function in the dish.
Jonghun Kim +2 more
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A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review
Stem Cell Research & Therapy, 2023 Background The first human brain organoid protocol was presented in the beginning of the previous decade, and since then, the field witnessed the development of many new brain region-specific models, and subsequent protocol adaptations and modifications.
Lance A. Mulder +5 more
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Electrophysiological Analysis of Brain Organoids: Current Approaches and Advancements
Frontiers in Neuroscience, 2021 Brain organoids, or cerebral organoids, have become widely used to study the human brain in vitro. As pluripotent stem cell-derived structures capable of self-organization and recapitulation of physiological cell types and architecture, brain organoids ...
Austin P. Passaro +4 more
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