Results 41 to 50 of about 26,410 (296)

Decellularised extracellular matrix-derived peptides from neural retina and retinal pigment epithelium enhance the expression of synaptic markers and light responsiveness of human pluripotent stem cell derived retinal organoids [PDF]

, 2019
Tissue specific extracellular matrices (ECM) provide structural support and enable access to molecular signals and metabolites, which are essential for directing stem cell renewal and differentiation.
Al-Aama, Jumana, Asfour, Hani Z., Ding, Yuchun, Doherty, Mary, Dorgau, Birthe, Felemban, Majed, Hallam, Dean, Hilgen, Gerrit, Hunt, Nicola Claire, Kiening, Martin, Krasnogor, Natalio, Lako, Majlinda, Sernagor, Evelyne, White, Kathryn, Whitfield, Phil, Zerti, Darin   +15 more
core   +2 more sources

Transcriptomic Mapping of Neural Diversity, Differentiation and Functional Trajectory in iPSC-Derived 3D Brain Organoid Models

Cells, 2021
Experimental models of the central nervous system (CNS) are imperative for developmental and pathophysiological studies of neurological diseases. Among these models, three-dimensional (3D) induced pluripotent stem cell (iPSC)-derived brain organoid ...
Kiavash Kiaee, Yasamin A. Jodat, Nicole J. Bassous, Navneet Matharu, Su Ryon Shin   +4 more
doaj   +1 more source

Human Brain Organoids as Models for Central Nervous System Viral Infection

Viruses, 2022
Pathogenesis of viral infections of the central nervous system (CNS) is poorly understood, and this is partly due to the limitations of currently used preclinical models. Brain organoid models can overcome some of these limitations, as they are generated
Josse A. Depla, Lance A. Mulder, Renata Vieira de Sá, Morgane Wartel, Adithya Sridhar, Melvin M. Evers, Katja C. Wolthers, Dasja Pajkrt   +7 more
doaj   +1 more source

3D-printed microplate inserts for long term high-resolution imaging of live brain organoids

BMC Biomedical Engineering, 2021
Background Organoids are a reliable model used in the study of human brain development and under pathological conditions. However, current methods for brain organoid culture generate tissues that range from 0.5 to 2 mm of size, which need to be ...
Mariana Oksdath Mansilla, Camilo Salazar-Hernandez, Sally L. Perrin, Kaitlin G. Scheer, Gökhan Cildir, John Toubia, Kristyna Sedivakova, Melinda N. Tea, Sakthi Lenin, Elise Ponthier, Erica C. F. Yeo, Vinay Tergaonkar, Santosh Poonnoose, Rebecca J. Ormsby, Stuart M. Pitson, Michael P. Brown, Lisa M. Ebert, Guillermo A. Gomez   +17 more
doaj   +1 more source

GSK3ß inhibitor CHIR 99021 modulates cerebral organoid development through dose-dependent regulation of apoptosis, proliferation, differentiation and migration.

PLoS ONE, 2021
Cerebral organoids generated from human pluripotent stem cells (hiPSCs) are unique in their ability to recapitulate human-specific neurodevelopmental events.
Chloe Delepine, Vincent A Pham, Hayley W S Tsang, Mriganka Sur   +3 more
doaj   +1 more source

Modeling neurological disorders using brain organoids

Seminars in Cell & Developmental Biology, 2021
Neurological disorders are challenging to study given the complexity and species-specific features of the organ system. Brain organoids are three dimensional structured aggregates of neural tissue that are generated by self-organization and differentiation from pluripotent stem cells under optimized culture conditions.
Daniel Y, Zhang, Hongjun, Song, Guo-Li, Ming   +2 more
openaire   +3 more sources

Buckling without bending: a new paradigm in morphogenesis

, 2018
A curious feature of organ and organoid morphogenesis is that in certain cases, spatial oscillations in the thickness of the growing "film" are out-of-phase with the deformation of the slower-growing "substrate," while in other cases, the oscillations ...
Engstrom, T. A., Joyner, A. L., Lawton, A. K., Schwarz, J. M., Zhang, Teng   +4 more
core   +1 more source

CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma. [PDF]

, 2020
BackgroundLong non-coding RNAs (lncRNAs) exhibit highly cell type-specific expression and function, making this class of transcript attractive for targeted cancer therapy.
Akeson, Mark, Birk, Harjus S, Gupta, Nalin, He, Daniel, Hong, Sung Jun, Horlbeck, Max A, Jain, Miten, Koontz, Mark, Lien, Brian V, Lim, Daniel A, Liu, S John, Malatesta, Martina, Monje, Michelle, Olsen, Hugh E, Pedraza, Leslie, Raleigh, David R, Saha, Parna, Seo, Kyounghee, Thombare, Shivani S, Ullian, Erik M, Weissman, Jonathan S   +20 more
core   +1 more source

Microglia innately develop within cerebral organoids

Nature Communications, 2018
Brain organoid models reported to date lack cells of mesodermal origin, such as microglia. Here, the authors demonstrate that mature microglia-like cells are generated within their cerebral organoid model, providing new avenues for studying human ...
Paul R. Ormel, Renata Vieira de Sá, Emma J. van Bodegraven, Henk Karst, Oliver Harschnitz, Marjolein A. M. Sneeboer, Lill Eva Johansen, Roland E. van Dijk, Nicky Scheefhals, Amber Berdenis van Berlekom, Eduardo Ribes Martínez, Sandra Kling, Harold D. MacGillavry, Leonard H. van den Berg, René S. Kahn, Elly M. Hol, Lot D. de Witte, R. Jeroen Pasterkamp   +17 more
doaj   +1 more source

Bioengineering an Artificial Human Blood–Brain Barrier in Rodents

Bioengineering, 2019
Our group has recently created a novel in-vivo human brain organoid vascularized with human iPSC-derived endothelial cells. In this review article, we discuss the challenges of creating a perfused human brain organoid model in an immunosuppressed rodent ...
Kimia Kamal, Ben Waldau
doaj   +1 more source