Establishment and application of a quadruple target real-time PCR assay for detecting Yersinia pestis. [PDF]
PLoS ONEIn order to establish an efficient method for high-throughput detection of Yersinia pestis (Y. pestis), a quadruple target real-time polymerase chain reaction assay was developed based on the specific target genes of Y.
Wenfang Wang +3 more
doaj +2 more sources
The CAF1-NOT complex of trypanosomes [PDF]
Frontiers in Genetics, 2014 In African trypanosomes, there is no control of transcription initiation by RNA polymerase II at the level of individual protein-coding genes. Transcription is polycistronic, and individual mRNAs are excised by trans splicing and polyadenylation.
Esteban eErben +2 more
doaj +3 more sources
PLoS Pathogens, 2019 With relatively few known specific transcription factors to control the abundance of specific mRNAs, Plasmodium parasites may rely more on the regulation of transcript stability and turnover to provide sufficient gene regulation.
Kevin J Hart +7 more
doaj +2 more sources
The intrinsic structure of poly(A) RNA determines the specificity of Pan2 and Caf1 deadenylases [PDF]
Nature Structural & Molecular Biology, 2019 The 3′ poly(A) tail of messenger RNA is fundamental to regulating eukaryotic gene expression. Shortening of the poly(A) tail, termed deadenylation, reduces transcript stability and inhibits translation.
T. Tang +3 more
semanticscholar +3 more sources
TDP-43 accelerates deadenylation of target mRNAs by recruiting Caf1 deadenylase
FEBS Letters, 2019 TAR DNA‐binding protein 43 (TDP‐43) is an RNA‐binding protein, whose loss‐of‐function mutation causes amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration.
Shinichi Hoshino, Nao Hosoda
exaly +2 more sources
Development of a Multiplex TaqMan Real-Time PCR Assay for the Simultaneous Detection and Differentiation of Three Pathogenic Yersinia Species [PDF]
Veterinary SciencesThree pathogenic species of the genus Yersinia, including Plague-associated Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica, are commonly associated with human infection.
Xue Yang +14 more
doaj +2 more sources
Dietary cauliflower (Brassica oleracea var. botrytis) mitigates benzo[a]pyrene-induced oxidative stress, immune dysfunction, and tissue damage in Nile tilapia [PDF]
Scientific ReportsBenzo[a]pyrene (BaP) is a ubiquitous polycyclic aromatic hydrocarbon that has been reported to induce immunotoxicity and oxidative stress in fish. In the current study, the potential protective efficacy of dietary cauliflower powder (CAF; Brassica ...
Zeinab El-Bouhy +5 more
doaj +2 more sources
Single-Cell Sequencing Data Revealed Mechanisms of Interactions Between Tumor Cells and Cancer-Associated Fibroblasts in Metastatic Colorectal Cancer. [PDF]
Biomed Res IntColorectal cancer (CRC) is highly metastatic, yet the interaction between tumor cells and microenvironment components remains unclear, as does its impact on patient outcomes. Single‐cell data (GSE225857 and GSE166555) were collected for transformed and primary CRC to identify differing cell types and their subpopulations. Prognostic signature genes for
Tan Y +8 more
europepmc +2 more sources
Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2-arm mRNA Platform. [PDF]
Adv Sci (Weinh)We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Teng X +5 more
europepmc +2 more sources
Insight into Interface Engineering at TiO2/Dye through Molecularly Functionalized Caf1 Biopolymer [PDF]
ACS Sustainable Chemistry & Engineering, 2017 The fast charge recombination kinetics and poor sensitizing ability in dye-sensitized solar cells (DSSCs) result in a significant electron loss and performance degradation.
S. Akin +4 more
semanticscholar +5 more sources