Results 51 to 60 of about 654,231 (380)

Cytosolic‐enhanced dark Epac‐based FRET sensors allow for intracellular cAMP detection in live cells via FLIM

FEBS Letters, EarlyView.
We describe a novel set of Epac‐based FRET‐FLIM biosensors with improved fully cytosolic distribution, achieved without compromising the state‐of‐the‐art performance of our original designs, for detecting cAMP dynamics in real time in live cells with high precision and reliability.
Giulia Zanetti, Jeffrey B. Klarenbeek, Kees Jalink   +2 more
wiley   +1 more source

Multiscale studies of delayed afterdepolarizations II: Calcium-overload-induced ventricular arrhythmias [PDF]

arXiv, 2023
Disturbances in calcium homeostasis in a cardiac myocyte can lead to calcium-overload conditions and abnormal calcium releases, which occur primarily in the following two phases of the action potential (AP): (a) triggered or late calcium release (LCR) during the plateau phase; (b) spontaneous calcium release (SCR) during the diastolic interval (DI ...
arxiv  

The cDNA and deduced amino acid sequence of the γ subunit of the L-type calcium channel from rabbit skeletal muscle [PDF]

, 1990
Complementary DNAs for the γ subunit of the calcium channel of rabbit skeletal muscle were isolated on the basis of peptide sequences derived from the purified protein.
Biel, Cornwell, E. Bosse, Ellis, Endicott, F. Hofmann, Feinberg, Flockerzi, Flockerzi, Frohman, H.E. Meyer, Kyte, M. Biel, Mikami, Morton, P. Ruth, Perez-Reyes, Rö, Ruth, S. Regulla, Saiki, Sambrook, Sharp, Sieber, Striessnig, Takahashi, Tanabe, Tanabe, Tanabe, V. Flockerzi, Vilven   +30 more
core   +1 more source

Calcium Channel Engineering [PDF]

Biophysical Journal, 2015
High-voltage-activated calcium (CaV) channels convert electrical signals into Ca2+ ion influx to trigger biological responses in excitable cells. CaV channel dysfunction underlies many cardiovascular and neurological diseases, from cardiac arrhythmias to autism. Modulation of CaV channels by intracellular signaling proteins, Ca2+ ions and various small
openaire   +2 more sources

The [2Fe‐2S] cluster of mitochondrial outer membrane protein mitoNEET has an O2‐regulated nitric oxide access tunnel

FEBS Letters, EarlyView.
The mitochondrial outer membrane iron–sulphur ([Fe‐S]) protein mitoNEET is a target of the type‐2 diabetes drug pioglitazone. Its unknown molecular function is linked to respiratory complex activity and mitochondrial function. We discovered that O2 protects the mitoNEET [2Fe‐2S] cluster against NO oxidation and desensitization towards reduction by H2S.
Thao Nghi Hoang, Meritxell Wu‐Lu, Alberto Collauto, Peter‐Leon Hagedoorn, Madalina Alexandru, Maike Henschel, Shahram Kordasti, Maria Andrea Mroginski, Maxie M. Roessler, Kourosh H. Ebrahimi   +9 more
wiley   +1 more source

A small footprint couch‐top support device for image‐guided radiotherapy of heavy patients

Journal of Applied Clinical Medical Physics, Volume 23, Issue 12, December 2022., 2022
Abstract Purpose Patients with body weights close to or above 400 lbs present unique challenges in radiation therapy since the weight limit of most treatment couches decreases as the couch‐top extends toward the treatment gantry. The purpose of this work was to develop a small footprint couch‐top support platform to safely perform image‐guided ...
Huixiao Chen, Kevin Morley, Ronnie Rodriguez, Emily Draeger, Muhammad Shafiq ul Hassan, Zhe (Jay) Chen   +5 more
wiley   +1 more source

Auxiliary subunit regulation of high-voltage activated calcium channels expressed in mammalian cells [PDF]

, 2004
The effects of auxiliary calcium channel subunits on the expression and functional properties of high-voltage activated (HVA) calcium channels have been studied extensively in the Xenopus oocyte expression system, but are less completely characterized in
Ball S.L., Bangalore R., Barclay J., Canti C., Castellano A., De Waard M., Felix R., Gerster U., Jones O.T., Kamp T.J., Klugbauer N., Neely A., Qian J., Qin N., Rousset M., Shistik E., Stephens G.J., Wakamori M., Williams M.E.   +18 more
core   +1 more source

Calcium channels and migraine

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2013
Missense mutations in CACNA1A, the gene that encodes the pore-forming α1 subunit of human voltage-gated Ca(V)2.1 (P/Q-type) calcium channels, cause a rare form of migraine with aura (familial hemiplegic migraine type 1: FHM1). Migraine is a common disabling brain disorder whose key manifestations are recurrent attacks of unilateral headache that may be
openaire   +5 more sources

The apo LETM1 F‐EF‐hand adopts a closed conformation that underlies a multi‐modal sensory role in mitochondria

FEBS Letters, EarlyView.
We present the first solution structure of the Ca2+‐depleted LETM1 F‐EF‐hand through a D676A/N678A Ca2+ binding‐deficient mutant, revealing a closed hydrophobic cleft caused by a unique F1‐helix pivot. The apo LETM1 F‐EF‐hand exhibits regiospecific hot and cold unfolding, sensitivity to physiological pH changes and potential for promiscuous heterotypic
Qi‐Tong Lin, Danielle M. Colussi, Peter B. Stathopulos   +2 more
wiley   +1 more source

Clinical commissioning of an adaptive radiotherapy platform: Results and recommendations

Journal of Applied Clinical Medical Physics, Volume 23, Issue 12, December 2022., 2022
Abstract Online adaptive radiotherapy platforms present a unique challenge for commissioning as guidance is lacking and specialized adaptive equipment, such as deformable phantoms, are rare. We designed a novel adaptive commissioning process consisting of end‐to‐end tests using standard clinical resources.
Kelly Kisling, Timothy D. Keiper, Daniela Branco, Grace Gwe‐Ya Kim, Kevin L Moore, Xenia Ray   +5 more
wiley   +1 more source