Results 101 to 110 of about 652,107 (224)

Imaging and recording subventricular zone progenitor cells in live tissue of postnatal mice

Frontiers in Neuroscience, 2010
The subventricular zone (SVZ) is one of two regions where neurogenesis persists in the postnatal brain. The SVZ, located along the lateral ventricle, is the largest neurogenic zone in the brain that contains multiple cell populations including astrocyte ...
Benjamin Lacar, Benjamin Lacar, Stephanie Z Young, Stephanie Z Young, Jean-Claude Platel, Jean-Claude Platel, Angelique Bordey, Angelique Bordey   +7 more
doaj   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Electrical control of calcium oscillations in mesenchymal stem cells using microsecond pulsed electric fields

Stem Cell Research & Therapy, 2017
Background Human mesenchymal stem cells are promising tools for regenerative medicine due to their ability to differentiate into many cellular types such as osteocytes, chondrocytes and adipocytes amongst many other cell types.
Hanna Hanna, Franck M. Andre, Lluis M. Mir   +2 more
doaj   +1 more source

MDL‐800‐induced SIRT6 activation decreases age‐associated osteoarthritis in mice

Arthritis &Rheumatology, Accepted Article.
Objective SIRT6 is a nuclear‐localized histone deacetylase that regulates multiple pathways associated with aging. This study aimed to determine whether systemic administration of a small molecule activator of SIRT6, MDL‐800, decreases the severity of age‐associated osteoarthritis (OA) in mice.
Matheus Moreira Perez, Pranay Ramteke, Bahiyah Watson, Owen Haslam, Eirene Choi, Susan Chubinskaya, Mandy J. Peffers, William R. Davis, Jacqueline Shine, Brian O. Diekman, Richard F. Loeser, Theresa A. Freeman, Makarand V. Risbud, John A. Collins   +13 more
wiley   +1 more source

Extracellular Vesicles‐Based Biological Macromolecule Delivery Systems: Biogenesis, Characterization, and Emerging Clinical Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are nanoscale membrane‐bound structures that play a pivotal role in intercellular communication by transporting bioactive molecules, including proteins, lipids, nucleic acids, and organelles, between cells. Originating from the endosomal pathway, EVs reflect the physiological and pathological status of their parent
Emine İncilay Torunoğlu, Zeynep Betül Sarı, Muhammed Emin Sarı, Erdi Can Aytar   +3 more
wiley   +1 more source

Figure S4-S6 from Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation

, 2017
Figure S4. Effect on skin above tumors treated with calcium electroporation MDA-MB231 (human breast cancer) tumors treated with different doses of calcium electroporation: 168 mM CaCl2 injected in a volume equivalent to 20% - 80% of tumor volume and 100 -
Stine K. Frandsen (14927444), Trine Tramm (236900), Uma M. Mangalanathan (14927450), Mie B. Krüger (14927447), Ivana Novak (293544), Faisal Mahmood (815679), Julie Gehl (61817)   +6 more
core   +1 more source

Combinatorial Metabolic Engineering of Alkane Biosynthesis in the Osmotolerant Yeast Debaryomyces hansenii CBS 767

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Debaryomyces hansenii is a yeast with diverse potential applications like producing oleochemicals from biomass. Yet, combinatorial metabolic engineering in D. hansenii is limited because, like many nonconventional organisms, key genomic data and genetic elements are missing. Here, we report phenotypic characterization, genomic integration loci,
Zekun Li, Nilesh Kumar Sharma, Sarah Weintraub, Eric Young   +3 more
wiley   +1 more source

Effects of Electroporation on the Function of Sarco/Endoplasmic Reticulum Ca²⁺-ATPase and Na⁺,K⁺-ATPase in H9c2 Cells

Applied Sciences
Pulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to leak through the permeabilized membrane.
Vid Jan, Maida Jusović, Damijan Miklavčič   +2 more
doaj   +1 more source

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Numerical assessments of geometry, proximity and multi-electrode effects on electroporation in mitochondria and the endoplasmic reticulum to nanosecond electric pulses

Scientific Reports
Most simulations of electric field driven bioeffects have considered spherical cellular geometries or probed symmetrical structures for simplicity. This work assesses cellular transmembrane potential build-up and electroporation in a Jurkat cell that ...
C. Baker, A. Willis, W. Milestone, M. Baker, A. L. Garner, R. P. Joshi   +5 more
doaj   +1 more source