Results 71 to 80 of about 652,107 (224)

Effect of Moderate Electric Fields on the Physical and Chemical Characteristics of Cheese Emulsions

Gels, 2023
Cheese powder is a multifunctional ingredient that is produced by spray drying a hot cheese emulsion called cheese feed. Feed stability is achieved by manipulating calcium equilibrium using emulsifying salts.
Ipek Altay, Lucas Sales Queiroz, Naaman F. Nogueira Silva, Aberham Hailu Feyissa, Federico Casanova, Jens J. Sloth, Mohammad Amin Mohammadifar   +6 more
doaj   +1 more source

Layer‐Specific Dermal and Subcutaneous Delivery via Modular LNP–Hydrogel‐Integrated Threaded Microneedles

Advanced Science, EarlyView.
Guided by spatial transcriptomics and clinical validation, a threaded microneedle platform enables precise layer‐specific delivery across the dermis and subcutaneous tissue. By addressing distinct pathogenic remodeling in different skin layers, this strategy coordinates compartment‐specific intervention, restores tissue architecture, and offers a ...
Fangzhou Xie, Yixiang Zhang, Shifeng Ling, Yuxuan Huang, Yingying Wei, Rao Fu, Jiahao He, Baikun Liu, Lucia Zhou, Kaifeng Huo, Yida Wang, Qingfeng Li, Yawei Du, Wenguo Cui, Yun Xie   +14 more
wiley   +1 more source

Improving the Repeatability and Efficacy of Intradermal Electroporated Self-Replicating mRNA

Molecular Therapy: Nucleic Acids, 2019
Local administration of naked self-replicating mRNA (sr-mRNA) in the skin or muscle using electroporation is effective but hampered by low repeatability.
Hanne Huysmans, Joyca De Temmerman, Zifu Zhong, Séan Mc Cafferty, Francis Combes, Freddy Haesebrouck, Niek N. Sanders   +6 more
doaj   +1 more source

Starting a Fire Without Flame: The Induction of Cell Death and Inflammation in Electroporation-Based Tumor Ablation Strategies

Frontiers in Oncology, 2020
New therapeutic strategies and paradigms are direly needed for the treatment of cancer. While the surgical removal of tumors is favored in most cancer treatment plans, resection options are often limited based on tumor localization.
Rebecca M. Brock, Natalie Beitel-White, Natalie Beitel-White, Rafael V. Davalos, Irving C. Allen, Irving C. Allen   +5 more
doaj   +1 more source

Upadacitinib Restrains the Pathogenic Fitness of CD4+ T Cells and Aberrant B Cell Programming in Optic Neuritis

Advanced Science, EarlyView.
Single‐cell profiling and functional perturbation reveal coordinated JAK1‐pSTAT3 downstream programs in optic neuritis, including MCL1‐dependent fitness of pathogenic CD4+ Tem cells and glycolysis‐linked, cholesterol‐sensitive B‐cell responses associated with RORA. Upadacitinib disrupts this reciprocal T‐B‐cell circuit and alleviates neuroinflammation,
Gengchen Jiang, Qi Jiang, Lian Han, Yue Peng, Xingyu Liu, Tong Yang, Hao Huang, Jiaying Liu, Yuan Gao, Hui Yang, Wenjun Zou, Zhaohuai Li, Wenru Su   +12 more
wiley   +1 more source

Calcium indicator loading of neurons using single-cell electroporation [PDF]

Pflügers Archiv - European Journal of Physiology, 2007
Studies of subcellular Ca(2+) signaling rely on methods for labeling cells with fluorescent Ca(2+) indicator dyes. In this study, we demonstrate the use of single-cell electroporation for Ca(2+) indicator loading of individual neurons and small neuronal networks in rat neocortex in vitro and in vivo.
Nevian, T., Helmchen, F.
openaire   +3 more sources

Design and Construction of a Programmable Electroporation system for Biological Applications

, 2007
Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control
Beeby, Steve, Rodamporn, Somphop, Chad, John, Harris, Nick, Brown, Andrew   +4 more
core   +1 more source

CRISPR and Gene Augmentation Rescue Trabecular Meshwork Dysfunction in iPSC Models of Lowe Syndrome

Advanced Science, EarlyView.
By modeling Lowe syndrome using patient‐derived iPSCs, this study establishes a human disease model that faithfully recapitulates OCRL deficiency‐associated ciliary and cytoskeletal defects. The model enables evaluation of both mutation‐agnostic DNA augmentation and CRISPR‐mediated mutation correction strategies, both of which restore OCRL function and
Siyu Chen, Zhiquan Liu, Wenmin Wang, Qing Wang, Tia J. Kowal, Fan Zhang, Grzegorz Walkiewicz, Faruk Hossen, Herbert M. Lachman, Jinqiong Zhou, Yiting Wang, Yang Sun   +11 more
wiley   +1 more source

Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging. [PDF]

PLoS ONE, 2014
The spinal dorsal horn comprises heterogeneous populations of interneurons and projection neurons, which form neuronal circuits crucial for processing of primary sensory information.
Kazuhiko Nishida, Shinji Matsumura, Wataru Taniguchi, Daisuke Uta, Hidemasa Furue, Seiji Ito   +5 more
doaj   +1 more source

Detaching Photosensitive Nanoparticles from Cells Prevents Them from Remaining in Cells after Photoporation

Advanced Science, EarlyView.
Phenylboronic acid‐functionalized gold nanoparticles reversibly attach to cell‐surface glycans, enhancing photoporation‐mediated intracellular delivery. After irradiation, ATP competitively disrupts the boronic ester interactions and removes cell‐associated nanoparticles, markedly reducing residual gold.
Tao Lu, Mina Nikolić, Baihao Huang, Deep Punj, Herlinde De Keersmaecker, Thibaut Van Acker, Wenjia Xie, Florian Vanlauwe, Phillip Blondeel, Liesl De Graeve, Félix Sauvage, Ranhua Xiong, Chaobo Huang, Frank Vanhaecke, Kevin Braeckmans, Stefaan C. De Smedt   +15 more
wiley   +1 more source