Results 61 to 70 of about 652,107 (224)

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Histone deacetylase 4 and 5 translocation elicited by microsecond pulsed electric field exposure is mediated by kinase activity

Frontiers in Bioengineering and Biotechnology, 2022
Electroporation-based technologies using microsecond pulsed electric field (µsPEF) exposures are established as laboratory and clinical tools that permeabilize cell membranes.
Zahra Safaei, Gary L. Thompson
doaj   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

Figure S3 from Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation

, 2017
Figure S3. Tumor necrosis after treatment with different doses of calcium electroporation MDA-MB231 (human breast cancer) tumors treated with different doses of calcium electroporation: 168 mM CaCl2 injected in a volume equivalent to 20% - 80% of tumor ...
Stine K. Frandsen (14927444), Trine Tramm (236900), Uma M. Mangalanathan (14927450), Mie B. Krüger (14927447), Ivana Novak (293544), Faisal Mahmood (815679), Julie Gehl (61817)   +6 more
core   +1 more source

Model of cell electrofusion Membrane electroporation, pore coalescence and percolation [PDF]

, 1987
Sugar IP, Förster W, Neumann E. Model of cell electrofusion Membrane electroporation, pore coalescence and percolation. Biophysical Chemistry.
Förster, Walter, Istvan P. Sugar, Eberhard Neumann, Sugar, Istvan P., Walter Förster, Neumann, Eberhard   +5 more
core   +1 more source

Dual‐Gene Edited Extracellular Vesicles Remodel the Redox Homeostasis to Inhibit Ferroptosis in Intervertebral Disc Degeneration

Advanced Science, EarlyView.
ABSTRACT Intervertebral disc degeneration (IDD) is driven by ferroptosis of nucleus pulposus cells (NPCs) as a core pathological mechanism. Nucleus pulposus progenitor cells (NPPCs), exhibiting stem cell‐like properties, yield extracellular vesicles (PEVs) with high affinity for NPCs and enable targeted phenotypic regulation.
Jing Yan, Yazhou Lin, Shuo Miao, Minghao Chao, Chengbai Dai, Ziwen Hao, Rui Chen, Ming Guan, Bin Pan, Fenglei Gao, Quan Zhou   +10 more
wiley   +1 more source

In vivo two-photon calcium imaging of cortical neurons in neonatal mice

STAR Protocols, 2023
Summary: In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level.
Takamitsu Egashira, Nao Nakagawa-Tamagawa, Elvira Abzhanova, Yuzuki Kawae, Ayami Kohara, Ryoko Koitabashi, Hiromi Mizuno, Hidenobu Mizuno   +7 more
doaj   +1 more source

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Dynamic Electroporation Modelling [PDF]

, 2009
In this thesis, modelling and simulation of the effects of electric fields on a single spherical cell are undertaken. Of interest is the effect that different electric field waveforms have on the induced transmembrane potential of the cell and by ...
Talele, Sadhana
core  

Bulk electroporation and population calcium imaging in the adult mammalian retina [PDF]

Journal of Neurophysiology, 2011
The optical recording of light-evoked activity in populations of neurons in the mammalian retina offers several benefits over the use of multielectrode arrays. However, population imaging has been hindered by the effective loading of synthetic fluorescent indicators, especially in the mature tissue.
Briggman, K., Euler, T.
openaire   +4 more sources