Results 41 to 50 of about 20,949 (129)
STAR Protocols, 2022 Summary: We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ...
Masayuki Sakamoto +5 more
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Molecular Pain, 2017 Chemical calcium indicators have been commonly used to monitor calcium (Ca 2+ ) activity in cell bodies, i.e., somata, of isolated dorsal root ganglion neurons.
Yong Chen, Li-Yen M Huang
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Frontiers in Neural Circuits, 2014 The zebrafish has various advantages as a model organism to analyze the structure and function of neural circuits but efficient viruses or other tools for fast gene transfer are lacking.
Ming eZou +6 more
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The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability
Applied Sciences, 2023 Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer.
Rūta Palepšienė +4 more
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Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation [PDF]
Cancer Research, 2017 Abstract Calcium electroporation may offer a simple general tool for anticancer therapy. Transient permeabilization of cancer cell membranes created by applying short, high-voltage pulses in tumors enables high calcium influxes that trigger cell death. In this study, we compared the relative sensitivity of different human tumor models
Frandsen, Stine K. +6 more
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Selective gene expression by postnatal electroporation during olfactory interneuron neurogenesis. [PDF]
PLoS ONE, 2008 Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they integrate into circuits, and their role in coding remain mysteries.
Alexander T Chesler +5 more
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Frontiers in Bioengineering and Biotechnology, 2022 Electroporation-based technologies using microsecond pulsed electric field (µsPEF) exposures are established as laboratory and clinical tools that permeabilize cell membranes.
Zahra Safaei, Gary L. Thompson
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Bulk electroporation and population calcium imaging in the adult mammalian retina [PDF]
Journal of Neurophysiology, 2011 The optical recording of light-evoked activity in populations of neurons in the mammalian retina offers several benefits over the use of multielectrode arrays. However, population imaging has been hindered by the effective loading of synthetic fluorescent indicators, especially in the mature tissue.
Briggman, K., Euler, T.
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In vivo two-photon calcium imaging of cortical neurons in neonatal mice
STAR Protocols, 2023 Summary: In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level.
Takamitsu Egashira +7 more
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Effect of Moderate Electric Fields on the Physical and Chemical Characteristics of Cheese Emulsions
Gels, 2023 Cheese powder is a multifunctional ingredient that is produced by spray drying a hot cheese emulsion called cheese feed. Feed stability is achieved by manipulating calcium equilibrium using emulsifying salts.
Ipek Altay +6 more
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