Results 51 to 60 of about 20,949 (129)

Improving the Repeatability and Efficacy of Intradermal Electroporated Self-Replicating mRNA

Molecular Therapy: Nucleic Acids, 2019
Local administration of naked self-replicating mRNA (sr-mRNA) in the skin or muscle using electroporation is effective but hampered by low repeatability.
Hanne Huysmans, Joyca De Temmerman, Zifu Zhong, Séan Mc Cafferty, Francis Combes, Freddy Haesebrouck, Niek N. Sanders   +6 more
doaj   +1 more source

Starting a Fire Without Flame: The Induction of Cell Death and Inflammation in Electroporation-Based Tumor Ablation Strategies

Frontiers in Oncology, 2020
New therapeutic strategies and paradigms are direly needed for the treatment of cancer. While the surgical removal of tumors is favored in most cancer treatment plans, resection options are often limited based on tumor localization.
Rebecca M. Brock, Natalie Beitel-White, Natalie Beitel-White, Rafael V. Davalos, Irving C. Allen, Irving C. Allen   +5 more
doaj   +1 more source

Calcium indicator loading of neurons using single-cell electroporation [PDF]

Pflügers Archiv - European Journal of Physiology, 2007
Studies of subcellular Ca(2+) signaling rely on methods for labeling cells with fluorescent Ca(2+) indicator dyes. In this study, we demonstrate the use of single-cell electroporation for Ca(2+) indicator loading of individual neurons and small neuronal networks in rat neocortex in vitro and in vivo.
Nevian, T., Helmchen, F.
openaire   +3 more sources

Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging. [PDF]

PLoS ONE, 2014
The spinal dorsal horn comprises heterogeneous populations of interneurons and projection neurons, which form neuronal circuits crucial for processing of primary sensory information.
Kazuhiko Nishida, Shinji Matsumura, Wataru Taniguchi, Daisuke Uta, Hidemasa Furue, Seiji Ito   +5 more
doaj   +1 more source

Milk of calcium renal cyst and Liesegang structures

Human Pathology: Case Reports, 2019
Liesegang rings are rare and very few cases reported in literature in association with malignancy. Although they have no biologic or clinical significance, unique morphology and unfamiliarity with these structures are often confused with malignancy ...
Prashant Bavi, Jagdish Butany, Korosh Khalili, Runjan Chetty   +3 more
doaj   +1 more source

Direct Therapeutic Applications of Calcium Electroporation to Effectively Induce Tumor Necrosis [PDF]

Cancer Research, 2012
Abstract Electroporation of cells with short, high-voltage pulses causes a transient permeabilization of cell membranes that permits passage of otherwise nonpermeating ions and molecules. In this study, we illustrate how electroporation with isotonic calcium can achieve highly effective cancer cell kill in vivo.
Frandsen, Stine Krog, Gissel, Hanne, Hojman, Pernille, Tramm, Trine, Eriksen, Jens, Gehl, Julie   +5 more
openaire   +4 more sources

Calcium electroporation in primary cutaneous marginal zone lymphoma [PDF]

JDDG: Journal der Deutschen Dermatologischen Gesellschaft, 2021
Stranzenbach, René (Dr. med.), Dörler, Martin (Dr. med.), Scholl, Lisa (Dr. med.), Bechara, Falk Georges (Prof. Dr. med.)   +3 more
openaire   +3 more sources

Enhanced non-viral gene delivery via calcium phosphate/DNA co-precipitates with low-voltage pulse electroporation in NK-92 cells for immunocellular therapy

APL Bioengineering
Achieving high cell transfection efficiency is essential for various cell types in numerous disease applications. However, the efficient introduction of genes into natural killer (NK) cells remains a challenge.
Che-Yung Kuan, I-Hsuan Yang, Chia-Ting Chang, Zhi-Yu Chen, Jhih-Ni Lin, Wei-Ting Kuo, Yu-Ying Lin, Andrew Yueh, Feng-Huei Lin   +8 more
doaj   +1 more source

Primary Neuronal Culture and Transient Transfection

Bio-Protocol
Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro.
Shun- Tseng, Peng- Chen, Eric Hwang
doaj   +1 more source

Electrochemotherapy for the treatment of basal cell tumours of the nasal planum in three cats

Journal of Feline Medicine and Surgery Open Reports
Case series summary Malignant basal cell tumours may be seen on or near the nasal planum in cats, and include basal cell carcinomas, which are common, and basosquamous carcinomas, which are rare.
Alvaro Salmoral, Chiara Penzo, Stephen Baines   +2 more
doaj   +1 more source