Results 51 to 60 of about 652,107 (224)

In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

STAR Protocols, 2022
Summary: We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ...
Masayuki Sakamoto, Keisuke Ota, Yayoi Kondo, Michiko Okamura, Hajime Fujii, Haruhiko Bito   +5 more
doaj   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Modelling single cell electroporation with bipolar pulse parameters and dynamic pore radii [PDF]

, 2010
We develop a model of single spherical cell electroporation and simulate spatial and temporal aspects of the transmembrane potential and pore radii as an effect of any form of applied electric field.
van Ekeran, Jethro, Talele, Sadhana, Gaynor, Paul, Cree, Michael J., Michael J. Cree, Jethro van Ekeran, Paul Gaynor, Sadhana Talele   +7 more
core   +1 more source

A simple and fast method to image calcium activity of neurons from intact dorsal root ganglia using fluorescent chemical Ca indicators

Molecular Pain, 2017
Chemical calcium indicators have been commonly used to monitor calcium (Ca 2+ ) activity in cell bodies, i.e., somata, of isolated dorsal root ganglion neurons.
Yong Chen, Li-Yen M Huang
doaj   +1 more source

Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications

Frontiers in Neural Circuits, 2014
The zebrafish has various advantages as a model organism to analyze the structure and function of neural circuits but efficient viruses or other tools for fast gene transfer are lacking.
Ming eZou, Ming eZou, Paul eDe Koninck, Paul eDe Koninck, Rachael L. Neve, Rainer W Friedrich, Rainer W Friedrich   +6 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The Influence of Calcium Ions on the Electrotransfer Efficiency of Plasmid DNA and Cell Viability

Applied Sciences, 2023
Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer.
Rūta Palepšienė, Martynas Maciulevičius, Paulius Ruzgys, Baltramiejus Jakštys, Saulius Šatkauskas   +4 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Selective gene expression by postnatal electroporation during olfactory interneuron neurogenesis. [PDF]

PLoS ONE, 2008
Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they integrate into circuits, and their role in coding remain mysteries.
Alexander T Chesler, Claire E Le Pichon, Jessica H Brann, Ricardo C Araneda, Dong-Jing Zou, Stuart Firestein   +5 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source