Results 41 to 50 of about 26,568 (204)

Accumulation of human full-length tau induces degradation of nicotinic acetylcholine receptor α4 via activating calpain-2

Scientific Reports, 2016
Cholinergic impairments and tau accumulation are hallmark pathologies in sporadic Alzheimer’s disease (AD), however, the intrinsic link between tau accumulation and cholinergic deficits is missing.
Yaling Yin, Yali Wang, Di Gao, Jinwang Ye, Xin Wang, Lin Fang, Dongqin Wu, Guilin Pi, Chengbiao Lu, Xin-Wen Zhou, Ying Yang, Jian-Zhi Wang   +11 more
doaj   +1 more source

Traumatic mechanical injury to the hippocampus in vitro causes regional caspase-3 and calpain activation that is influenced by NMDA receptor subunit composition

Neurobiology of Disease, 2006
Apoptotic or necrotic cell death in the hippocampus is a major factor underlying the cognitive impairments following traumatic brain injury. In this study, we examined if traumatic mechanical injury would produce regional activation of calpain and ...
Michael N. DeRidder, Melissa J. Simon, Robert Siman, Yves P. Auberson, Ramesh Raghupathi, David F. Meaney   +5 more
doaj   +1 more source

A 3D Human Neuron‐on‐Chip Platform to Monitor Neuronal Injury Responses

Advanced Healthcare Materials, EarlyView.
This study presents a novel 3D Neuron‐on‐Chip model that can maintain human PSC‐derived excitatory prefrontal cortex neurons in 3D hydrogels and can be used to monitor neuronal injury responses over time. Results show injury‐induced acute neuronal excitotoxicity, declining neuronal connectivity, and the activation of a neurodegenerative, SASP‐like ...
Ruiping Tang, Charles‐Francois Latchoumane, Avi Chopra, Md Marzan Sarkar, Chunki Kim, Nathan Gonsalves, Hsueh‐Fu Wu, John C. Sentmanat, Alan Liu, Isha Mhatre‐Winters, Aditya Mishra, Andrei G. Fedorov, Jay M. Patel, Nadja Zeltner, Steven L. Stice, Jason R. Richardson, Lohitash Karumbaiah   +16 more
wiley   +1 more source

Mechanical Activation of Piezo1 Drives Osteoarthritis Through Kdm5c‐Mediated Epigenetic Silencing

Advanced Science, EarlyView.
Excessive mechanical stress activates Piezo1, triggering Ca2+‐dependent cytoskeletal forces that deform the nucleus and reduce H3K4me3. Kdm5c demethylates H3K4me3 at Col2a1 and Runx3 promoters. Kdm5c knockout rescues degradation. Repurposed telmisartan directly inhibits Kdm5c, blocking this axis and showing disease‐modifying efficacy in mouse OA models
Tianyou Kan, Xuran Li, Han Wang, Lin Sun, Yao Wang, Jiangdong Wu, Junqi Cui, Yiqi Yang, Kai Yuan, Linyang Chu, Liao Wang, Hanjun Li, Mengning Yan, Zhifeng Yu   +13 more
wiley   +1 more source

Deconstruction of Human Age‐Related Cataract Capsules Defines Aging

Advanced Science, EarlyView.
We generate a comprehensive atlas of age‐related cataracts (ARC) at a single‐cell resolution, encompassing three disease states—mild cataract group (Mild), severe cortical cataract group (Severe_C), and severe nuclear cataract group (Severe_N). We find that ARC involves seven distinct lens capsule cell types, with notable differences in cellular ...
Qiaomei Tang, Ziyang Tong, Chunmei Fan, Silong Chen, Jiarui Guo, Jianghua Hu, Ke Yao, Zi Yin, Xiao Chen, Yibo Yu   +9 more
wiley   +1 more source

Calpain inhibition promotes the rescue of F(508)del-CFTR in PBMC from cystic fibrosis patients.

PLoS ONE, 2013
A basal calpain activity promotes the limited proteolysis of wild type (WT) cystic fibrosis conductance regulator (CFTR), inducing the internalization of the split channel. This process contributes to the regulation in the level of the active CFTR at the
Monica Averna, Marco Pedrazzi, Laura Minicucci, Roberta De Tullio, Federico Cresta, Franca Salamino, Sandro Pontremoli, Edon Melloni   +7 more
doaj   +1 more source

Peripheral Blood DNA Methylation Changes Precede Lymphoma Diagnosis in Primary Sjögren's Disease

Arthritis &Rheumatology, EarlyView.
Objective Primary Sjögren's disease (SjD) is a systemic autoimmune disease associated with an increased risk of lymphoma. The molecular mechanisms underlying lymphomagenesis remain poorly understood, and sensitive biomarkers for early identification of patients at high risk of developing lymphoma are lacking.
Hanna Lidberg, Cecilia Fugmann, Juliana Imgenberg‐Kreuz   +2 more
wiley   +1 more source

Mutations in Calpain 3 Associated with Limb Girdle Muscular Dystrophy: Analysis by Molecular Modeling and by Mutation in m-Calpain [PDF]

Biophysical Journal, 2001
Limb-girdle muscular dystrophy type 2A (LGMD2A) is an autosomal recessive disorder characterized by selective atrophy of the proximal limb muscles. Its occurrence is correlated, in a large number of patients, with defects in the human CAPN3 gene, a gene that encodes the skeletal muscle-specific member of the calpain family, calpain 3 (or p94).
Jia, Zongchao, Petrounevitch, Vitali, Wong, Andrew, Moldoveanu, Tudor, Davies, Peter L., Elce, John S., Beckmann, Jacques S.   +6 more
openaire   +2 more sources

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

Regulation of Cell Adhesion and Migration by Kindlin-3 Cleavage by Calpain [PDF]

Journal of Biological Chemistry, 2012
Integrin activation on hematopoietic cells is essential for platelet aggregation, leukocyte adhesion, and transmigration through endothelium and extracellular matrix into inflamed tissues. To migrate through matrix, leukocyte integrin adhesion complexes undergo dynamic changes.
Yongzhong, Zhao, Nikolay L, Malinin, Julia, Meller, Yi, Ma, Xiaoxia Z, West, Kamila, Bledzka, Jun, Qin, Eugene A, Podrez, Tatiana V, Byzova   +8 more
openaire   +2 more sources