Results 11 to 20 of about 4,120 (216)
Comprehensive behavioral phenotyping of calpastatin-knockout mice
Molecular Brain, 2008 Background Calpastatin is an endogenous inhibitor of calpain, intracellular calcium-activated protease. It has been suggested to be involved in molecular mechanisms of long-term plasticity and excitotoxic pathways.
Nakajima Ryuichi +6 more
doaj +2 more sources
Differential regulation of the calpain–calpastatin complex by the L-domain of calpastatin
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2014 Here we demonstrate that the presence of the L-domain in calpastatins induces biphasic interaction with calpain. Competition experiments revealed that the L-domain is involved in positioning the first inhibitory unit in close and correct proximity to the calpain active site cleft, both in the closed and in the open conformation.
DE TULLIO, ROBERTA +6 more
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Isolation of Endogenous Calpastatin
, 2019 We here describe the purification of calpastatin from human erythrocytes. When calpastatin is purified from tissues, it is necessary to measure its inhibitory activity against calpain in the presence of Ca2+ to specifically identify the protein. Thus, the purification steps necessary to obtain the inhibitor protein were originally designed to obtain ...
De Tullio, Roberta, Averna, Monica
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Immunoblotting for Calpastatin Expression
, 2019 Immunoblotting is a procedure routinely used to analyze calpastatin expression. However, immunoblotting alone may not be adequate for this task, since calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications.
Averna, Monica, De Tullio, Roberta
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Properties of calpastatin forms in rat brain [PDF]
FEBS Letters, 1998 Four recombinant calpastatin forms, deduced from rat brain mRNAs and differing in the number of inhibitory repetitive domains from zero to four, were expressed and characterized for their inhibitory efficiency on μ‐ and m‐calpain. Although the most effective one is a truncated calpastatin form composed of the N‐terminal region (domain L) and a single ...
MELLONI, EDON +6 more
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Calpain‐calpastatin and fusion [PDF]
FEBS Letters, 1986 Rat erythrocytes fuse when treated with the membrane mobility agent, 2‐(2‐methoxyethoxy)ethyl‐cis‐8‐(2‐octylcyclopropyl) octanoate (A2C) and Ca2+, whereas human cells do not. Membrane proteolysis promoted by calpain is required for rat cell fusion [(1986) Eur. J. Biochem., in press].
Glaser, Tova, Kosower, Nechama S.
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Production and Purification of Recombinant Calpastatin
, 2019 The production of recombinant calpastatin in E. coli has become an efficient tool to obtain discrete amounts of a specific calpastatin species that can be present concomitantly with other calpastatin fragments/forms in the same tissue or cell type in a given condition.
De Tullio, Roberta, Averna, Monica
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Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters
Archives of Biochemistry and Biophysics, 2004 Calpastatin is the specific endogenous inhibitor of calpain proteinase that is encoded by a single gene. Transient transfection assays in both a non-fusing skeletal muscle and non-muscle cell-line demonstrated that the putative porcine calpastatin promoter regions 5' to exons 1xa, 1xb, and 1u were functional.
Tim, Parr +5 more
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Calpastatin level in spontaneously hypertensive rats
Biochemical and Biophysical Research Communications, 1991 We studied calpastatin activity in erythrocytes of Milan hypertensive and prehypertensive rats, in their normotensive controls, in F1 and F2 hybrids, and in two inbred strains derived from F2, one hypertensive and the other normotensive. Our results show that the decrease in calpastatin activity observed in Milan hypertensive rats was not caused by ...
L. Soldati +6 more
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Targeting NLRP3 Inflammasomes in Myocarditis: Potential Therapeutic Strategies and Clinical Translation. [PDF]
Scientifica (Cairo)Myocarditis can be caused by viral infections, systematic autoimmune diseases, and as a reaction to prescribed medications, and the nucleotide‐binding domain leucine‐rich repeat pyrin domain‐containing 3 (NLRP3) inflammasome is implicated in its pathophysiology.
Retnowati FD +3 more
europepmc +2 more sources