Results 51 to 60 of about 2,875 (178)

Effect of calcium chloride injection and freezing prior to wet aging on the meat quality of cull cows

Journal of the Science of Food and Agriculture, Volume 106, Issue 6, Page 3478-3486, April 2026.
Abstract BACKGROUND In Brazil, the slaughter of female cattle, particularly older animals, has increased substantially. However, meat from this category presents limitations in terms of quality, mainly because of its toughness resulting from a high concentration of cross‐links between collagen fibers.
Juliano CC Belmonte, Taynara S Santana, Micheline F Castro, Rosileide V Rohod, Marcelo Vedovatto, Aylpy RD Santos, Henrique J Fernandes, Dalton M Oliveira   +7 more
wiley   +1 more source

Reactive Oxygen Species: Molecular Mechanisms, Cellular Targets, and Implications for Genomic Stability

BioMed Research International, Volume 2026, Issue 1, 2026.
Reactive oxygen species (ROS) are highly reactive molecules generated through endogenous metabolic pathways and exogenous environmental exposures. While essential for physiological processes—including cell signaling, proliferation, differentiation, immune defense, and neurotransmission—dysregulated ROS production contributes to oxidative stress and ...
Alkhaddour Aziz, Zam Wissam, Poorani Gurumallesh Prabu   +2 more
wiley   +1 more source

Ex vivo measurement of calpain activation in human peripheral blood lymphocytes by detection of immunoreactive products of calpastatin degradation.

Folia Histochemica et Cytobiologica, 2008
Limited proteolysis of multiple intracellular proteins by endogenous Ca-dependent cysteine proteases--calpains--is an important regulatory mechanism for cell proliferation, apoptosis etc.
Jacek M Witkowski, Ewa Bryl, Andrzej Hellman, Andrzej Mital, Paulina Lopatniuk, Zaneta Smolenska, Agnieszka Daca, Zofia Puchalska, Anna Zaremba, Anna Mikosik   +9 more
doaj   +1 more source

Accurate Generation of Conformational Ensembles for Intrinsically Disordered Proteins with IDPFold

Advanced Science, Volume 12, Issue 48, December 29, 2025.
In brief: IDPFold predicts the conformational ensembles of intrinsically disordered proteins (IDPs) from their amino acid sequences. The atomic structures generated by IDPFold offer crucial insights into the intricate relationship between IDP structures and their diverse biological functions.2 Abstract Intrinsically disordered proteins (IDPs) play ...
Junjie Zhu, Zhengxin Li, Zhuoqi Zheng, Bo Zhang, Bozitao Zhong, Jie Bai, Xiaokun Hong, Taifeng Wang, Ting Wei, Jianyi Yang, Hai‐Feng Chen   +10 more
wiley   +1 more source

Peeling Skin, Leukonychia, Acral Punctate Keratoses, Cheilitis and Knuckle Pads (PLACK) Syndrome: An Updated Review of Cases and Identification of a Recurrent CAST Variant in Two Patients

Pediatric Dermatology, Volume 42, Issue 6, Page 1239-1247, November/December 2025.
ABSTRACT Peeling skin, leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK) syndrome (OMIM616295) is an exceptionally rare autosomal recessive genodermatosis caused by loss‐of‐function pathogenic variants in the CAST gene, encoding calpastatin.
Fiona Haxho, Richard M. Haber, Janan Mohamad, Ofer Sarig, Eli Sprecher, Renée Perrier, Dana Boctor, Michele Ramien   +7 more
wiley   +1 more source

Adipocyte Calpain‐2 Deficiency Reduces Obesity‐Accelerated Abdominal Aortic Aneurysm Formation in Mice

FASEB BioAdvances, Volume 7, Issue 10, October 2025.
Adipocyte‐specific deletion of calpain‐2 in mice decreases obesity‐accelerated abdominal aortic aneurysm formation, which is associated with reduced aortic medial elastin fragmentation and periaortic adipose tissue leukocyte accumulation. Targeted inhibition of calpain‐2 may offer a new therapeutic target to reduce obesity‐accelerated abdominal aortic ...
Ana Clara Frony, Aida Javidan, Weihua Jiang, Michihiro Okuyama, Lihua Yang, Haruhito A. Uchida, Venkateswaran Subramanian   +6 more
wiley   +1 more source

Dry‐Aged Beef: A Global Review of Meat Quality Traits, Microbiome Dynamics, Safety, and Sustainable Strategies

Journal of Food Science, Volume 90, Issue 10, October 2025.
ABSTRACT Dry‐aged beef is valued for its tenderness, complex aroma, and concentrated flavor. However, variability in aging protocols and limited understanding of underlying biological and technological processes can compromise consistent quality and safety.
Ana J. Ribeiro, Filipe Silva, Paula Teixeira, Cristina M. Saraiva   +3 more
wiley   +1 more source

Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin. [PDF]

PLoS ONE, 2017
Calpains are calcium-activated proteases that have biomedical and biotechnological potential. Their activity is tightly regulated by their endogenous inhibitor, calpastatin that binds to the enzyme only in the presence of calcium. Conventional approaches
Hung Huy Nguyen, Mihaly Varadi, Peter Tompa, Kris Pauwels   +3 more
doaj   +1 more source

Effect of Ca2+ on binding of the calpains to calpastatin

Journal of Biological Chemistry, 1989
Autolyzed mu-calpain, unautolyzed mu-calpain, autolyzed m-calpain, and unautolyzed m-calpain (mu-calpain is the micromolar Ca2+-requiring proteinase, m-calpain is the millimolar Ca2+-requiring proteinase) were passed through a calpastatin-affinity column at different free Ca2+ concentrations, and binding of the calpains to calpastatin was compared with
H P, Kapprell, D E, Goll
openaire   +2 more sources

Extracellular Vesicles Derived From the Feces of Pregnant Women Modulate T Cells Toward a Pregnancy‐Supportive Phenotype In Vitro

European Journal of Immunology, Volume 55, Issue 9, September 2025.
Fecal extracellular vesicles (fEVs) were isolated from preserved stool samples in DNA/RNA shield and characterized. In an in vitro model with PBMC, fEVs of pregnant women modulate T cells toward a regulatory phenotype favorable for pregnancy compared to fEVs of non‐pregnant women. ABSTRACT Pregnancy requires immune tolerance to a semi‐allogeneic fetus,
Stefanie Dietz‐Ziegler, Samantha Kewitz, Gabriele Kaiser, Jessica Rühle, Alexander Marmé, Alexander Dalpke, Bachar Cheaib, Jan Pauluschke‐Fröhlich, Melanie Henes, Ana Velic, Andreas Pich, Anneli Vollert, Martin Schaller, Felix Knab, Trim Lajqi, Christian F. Poets, Christian Gille, Natascha Köstlin‐Gille   +17 more
wiley   +1 more source