Results 191 to 200 of about 17,822 (241)

Increased campesterol synthesis by improving lipid content in engineered Yarrowia lipolytica

Applied Microbiology and Biotechnology, 2020
Sterols attract increasing attention due to their important bioactivities. The oleaginous yeast Yarrowia lipolytica has large lipid droplets, which provide storage for the accumulated steroid compounds. In this study, we have successfully constructed a campesterol biosynthetic pathway by modifying the synthetic pathway of ergosterol in Y.
Y. Qian, Si Yuan Tan, G. Dong, Y. Niu, C. Hu, Y. Meng   +5 more
semanticscholar   +3 more sources

Improved campesterol production in engineered Yarrowia lipolytica strains

Biotechnology Letters, 2017
To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids).By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis.
Yu Zhang, Ying Wang, Mingdong Yao, Hong Liu, Xiao Zhou, Wenhai Xiao, Yingjin Yuan   +6 more
semanticscholar   +3 more sources

Campesterol Semi‐Synthetic Derivatives as Potential Antibacterial: in vitro and in silico Evaluation

Chemistry & Biodiversity, 2023
In this study, twelve campesterol derivatives (2–13) were prepared by esterification reaction at the hydroxy group in C‐3 and catalytic hydrogenation at the carbon‐carbon double bond in C‐5(6). All obtained compounds were characterized by IR, 1H‐NMR, 13C‐
Francisco Erivaldo Freitas da Silva, Francisco das Chagas Lima Pinto, Otília Deusdênia Loiola Pessoa, Aluísio Marques da Fonseca, José Galberto Martins da Costa, Gilvandete Maria Pinheiro Santiago   +5 more
semanticscholar   +3 more sources

Combining Metabolic Engineering and Lipid Droplet Assembly to Achieve Campesterol Overproduction in Saccharomyces cerevisiae.

Journal of Agricultural and Food Chemistry
Campesterol is a kind of important functional food additive. Therefore, stable and efficient campesterol biosynthesis is significant. Herein, we first knocked out the sterol 22-desaturase gene in Saccharomyces cerevisiae and expressed sterol Δ7-reductase
Zhijie Qin, Yunliang Zhang, Song Liu, Weizhu Zeng, Jingwen Zhou, Sha Xu   +5 more
semanticscholar   +3 more sources

Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities

Phytotherapy Research, 2007
AbstractCampesterol, a plant sterol in nature, is known to have cholesterol lowering and anticarcinogenic effects. Since angiogenesis is essential for cancer, it was surmised that an antiangiogenic effect may be involved in the anticancer action of this compound. This study investigated the effect of campesterol on basic fibroblast growth factor (bFGF)‐
J. Choi, Eun-ok Lee, Hyo-Jung Lee, Kwan-Hyun Kim, K. Ahn, B. Shim, Nam-il Kim, Myoung‐Chong Song, N. Baek, Sung-Hoon Kim   +9 more
semanticscholar   +3 more sources

Biological variation of β-sitosterol, campesterol, and lathosterol as cholesterol absorption and synthesis biomarkers.

Clinica Chimica Acta, 2014
The analysis of blood for β-sitosterol and campesterol is the measures of cholesterol absorption while lathosterol is a measure of cholesterol synthesis.The biological variability of β-sitosterol, campesterol, and lathosterol was measured using liquid-chromatography tandem mass spectrometry from a cohort of 25 apparently healthy subjects, where blood ...
A. Wu, Weiming Ruan, J. Todd, K. Lynch
semanticscholar   +3 more sources

Increased flux of the plant sterols campesterol and sitosterol across a disrupted blood brain barrier

Steroids, 2015
The intact blood-brain barrier in mammalians prevents exchange of cholesterol loaden particles between periphery and brain and thus nearly all cholesterol in this organ originates from de novo synthesis. Dietary cholesterol homologues from plants, campesterol and sitosterol, are known to get enriched to some extent in the mammalian brain.
A. Saeed, G. Genové, Tian-hang Li, Frank Hülshorst, C. Betsholtz, I. Björkhem, D. Lütjohann   +6 more
semanticscholar   +3 more sources

A Virtual Drug Discovery Screening Illuminates Campesterol as a Potent Estrogen Receptor Alpha Inhibitor in Breast Cancer.

Journal of Medicinal Chemistry
Breast cancer remains a global health challenge, and innovative strategies are required to target estrogen receptor α (ERα), a key player in its development. This study investigates the potential of campesterol, a natural phytosterol, as an ERα inhibitor
Ranabir Majumder, Shreya Banerjee, Madhurima Mandal, Sucharita Patra, Subhasis Das, Mahitosh Mandal   +5 more
semanticscholar   +3 more sources

Detection of Argan Oil Adulteration Using Quantitative Campesterol GC-Analysis

Journal of the American Oil Chemists' Society, 2007
AbstractDetection of edible oil adulteration is of utmost important to ensure product quality and customer protection. Campesterol, a sterol found in seed oils, represents less than 0.4% of argan oil total sterol content. Quantitative analysis of campesterol by gas chromatography of argan oil and of a mixture of argan oil and readily commercially ...
M. Hilali, Z. Charrouf, A. Soulhi, L. Hachimi, D. Guillaume   +4 more
semanticscholar   +2 more sources

β-Sitosterol and campesterol stabilisation by natural and synthetic antioxidants during heating

Food Chemistry, 2011
Abstract The aim of this study was to estimate the effect of natural and synthetic antioxidants in protecting phytosterols during heating at 180 °C. Green tea extract, rosemary extract, a mix of tocopherols from rapeseed oil, a mix of synthetic tocopherols, phenolic compounds extracted from rapeseed meal, sinapic acid and BHT were used.
D. Kmiecik, J. Korczak, M. Rudzińska, J. Kobus‐Cisowska, A. Gramza-Michałowska, M. Hęś   +5 more
semanticscholar   +2 more sources