Fortification and bioaccessibility of saffron apocarotenoids in potato tubers
Carotenoids are C40 isoprenoids with well-established roles in photosynthesis, pollination, photoprotection, and hormone biosynthesis. The enzymatic or ROS-induced cleavage of carotenoids generates a group of compounds named apocarotenoids, with an ...
Lourdes Gómez Gómez +20 more
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Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis [PDF]
Sarah Frusciante +2 more
exaly +2 more sources
Characterization of a Novel Carotenoid Cleavage Dioxygenase from Plants [PDF]
The plant hormone abscisic acid is derived from the oxidative cleavage of a carotenoid precursor. Enzymes that catalyze this carotenoid cleavage reaction, nine-cis epoxy-carotenoid dioxygenases, have been identified in several plant species. Similar proteins, whose functions are not yet known, are present in diverse organisms.
S H, Schwartz, X, Qin, J A, Zeevaart
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Natural overexpression of CAROTENOID CLEAVAGE DIOXYGENASE 4 in tomato alters carotenoid flux
Abstract Carotenoids and apocarotenoids function as pigments and flavor volatiles in plants that enhance consumer appeal and offer health benefits. Tomato (Solanum lycopersicum.) fruit, especially those of wild species, exhibit a high degree of natural variation in carotenoid and apocarotenoid contents.
Hee Ju Yoo +6 more
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Preparation of carotenoid cleavage dioxygenases for X-ray crystallography
Carotenoid cleavage dioxygenases (CCDs) constitute a superfamily of enzymes that are found in all domains of life where they play key roles in the metabolism of carotenoids and apocarotenoids as well as certain phenylpropanoids such as resveratrol. Interest in these enzymes stems not only from their biological importance but also from their remarkable ...
Daruwalla, Anahita +2 more
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Enabling technologies in synthetic biology now present the opportunity to engineer wine yeast for enhanced novel aromas. In doing so, improved wine products will increase the desirability of wine for the consumer and add value to the winemaker.
John J. B. Timmins +4 more
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Evidence for distinct rate-limiting steps in the cleavage of alkenes by carotenoid cleavage dioxygenases [PDF]
Carotenoid cleavage dioxygenases (CCDs) use a nonheme Fe(II) cofactor to split alkene bonds of carotenoid and stilbenoid substrates. The iron centers of CCDs are typically five-coordinate in their resting states, with solvent occupying an exchangeable site.
Nimesh Khadka +5 more
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Genomewide Analysis of Carotenoid Cleavage Dioxygenases in Unicellular and Filamentous Cyanobacteria [PDF]
Carotenoid cleavage dioxygenases (CCDs) are a group of enzymes that catalyze the oxidative cleavage steps from carotenoids to various carotenoid cleavage products. Someccdgenes have been identified and encoded enzymes functionally characterized in many higher plants, but little in cyanobacteria.
Cui, Hongli, Wang, Yinchu, Qin, Song
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New insight into the cleavage reaction of Nostoc sp. strain PCC 7120 carotenoid cleavage dioxygenase in natural and nonnatural carotenoids. [PDF]
ABSTRACT Carotenoid cleavage dioxygenases (CCDs) are enzymes that catalyze the oxidative cleavage of carotenoids at a specific double bond to generate apocarotenoids. In this study, we investigated the activity and substrate preferences of NSC3, a CCD of Nostoc sp. strain PCC 7120, in vivo
Heo J, Kim SH, Lee PC.
europepmc +4 more sources
Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes [PDF]
Although a number of plant carotenoid cleavage dioxygenase (CCD) genes have been functionally characterized in different plant species, little is known about the biochemical role and enzymatic activities of members of the subclass 4 (CCD4). To gain insight into their biological function, CCD4 genes were isolated from apple (Malus x domestica, MdCCD4 ...
Huang, Fong-Chin +2 more
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