Results 161 to 170 of about 1,031,105 (176)

Deadenylation of cytoplasmic mRNA by the mammalian Ccr4–Not complex

Biochemical Society Transactions, 2012
The Ccr4–Not complex is one of the major deadenylase factors present in eukaryotic cells. This multi-subunit protein complex is composed of at least seven stably associated subunits in mammalian cells including two enzymatic deadenylase subunits: one DEDD (Asp-Glu-Asp-Asp)-type deadenylase (either CNOT7/human Caf1/Caf1a or CNOT8/human Pop2/Caf1b/Calif)
Rachel, Doidge, Saloni, Mittal, Akhmed, Aslam, G Sebastiaan, Winkler   +3 more
openaire   +2 more sources

Dissecting the Role of the Ccr4–Not Deadenylase Complex in Pluripotency and Differentiation

2023
In this chapter, we describe methods and assays to examine the role of the Ccr4-Not mRNA deadenylase complex in regulating mouse embryonic stem cell (ESC) pluripotency and differentiation. We present the following procedures: sgRNA design and cloning, the culture of mouse ESCs, transfection and colony picking, genotyping, generation of Cnot3 ...
Xiukun, Wang, Qing, Chen, Brad, Lackford, Guang, Hu   +3 more
openaire   +2 more sources

The CCR4–NOT Complex Plays Diverse Roles in mRNA Metabolism

2003
It is increasingly clear that the synthesis of eukaryotic mRNA involves an integrated series of events involving large multisubunit protein complexes. The evolutionarily conserved CCR4-NOT complex of proteins has been found to be involved in several aspects of mRNA formation, including repression and activation of mRNA initiation, control of mRNA ...
Clyde L, Denis, Junji, Chen
openaire   +2 more sources

Etude structurale du complexe CCR4-NOT

Le recyclage des ARN débute par un étape de déadenylation ou la queue poly (A) est enzymatiquement clivée. La deadenylation est l’étape limitante dans le processus de dégradation des ARN. In vivo la deadenylation s’effectue successivement par les complexes multi-protéiques Pan2-Pan3 et Ccr4-Not.
openaire   +1 more source

Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex 1 1Edited by D. Draper

Journal of Molecular Biology, 2001
Junji Chen, Juri Rappsilber, Yueh-Chin Chiang   +2 more
exaly  

Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex

EMBO Journal, 2002
Hiroyuki Hanzawa, Martine A Collart, Rolf Boelens   +2 more
exaly  

Multifunctional roles of the mammalian CCR4–NOT complex in physiological phenomena

Frontiers in Genetics, 2014
Yo-Taro Shirai, Toru Suzuki, Masahiro Morita   +2 more
exaly  

PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay

Molecular Cell, 2018
Juhyuk Park, Minju Ha, JAECHUL Lim
exaly  

The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively

EMBO Journal, 1998
Juri Rappsilber, Matthias Mann, Clyde L Denis   +2 more
exaly  

Characterization of Mutations in NOT2 Indicates that it Plays an Important Role in Maintaining the Integrity of the CCR4–NOT Complex

Journal of Molecular Biology, 2002
Pamela J Russell, Clyde L Denis
exaly