Results 21 to 30 of about 13,668 (163)

Ccr4-Not complex reduces transcription efficiency in heterochromatin. [PDF]

Nucleic Acids Res, 2022
Abstract Heterochromatic silencing is thought to occur through a combination of transcriptional silencing and RNA degradation, but the relative contribution of each pathway is not known. In this study, we analyzed RNA Polymerase II (RNA Pol II) occupancy and levels of nascent and steady-state RNA in different mutants of ...
Monteagudo-Mesas P, Brönner C, Kohvaei P, Amedi H, Canzar S, Halic M.   +5 more
europepmc   +4 more sources

The CCR4-NOT complex is a tumor suppressor in Drosophila melanogaster eye cancer models [PDF]

Journal of Hematology & Oncology, 2018
Background The CNOT3 protein is a subunit of the CCR4-NOT complex, which is involved in mRNA degradation. We recently identified CNOT3 loss-of-function mutations in patients with T-cell acute lymphoblastic leukemia (T-ALL). Methods Here, we use different
Carmen Vicente, Rocco Stirparo, Sofie Demeyer, Charles E. de Bock, Olga Gielen, Mardelle Atkins, Jiekun Yan, Georg Halder, Bassem A. Hassan, Jan Cools   +9 more
doaj   +2 more sources

Translation efficiency driven by CNOT3 subunit of the CCR4-NOT complex promotes leukemogenesis [PDF]

Nature Communications
Protein synthesis is frequently deregulated during tumorigenesis. However, the precise contexts of selective translational control and the regulators of such mechanisms in cancer is poorly understood.
Maryam Ghashghaei, Yilin Liu, James Ettles, Giuseppe Bombaci, Niveditha Ramkumar, Zongmin Liu, Leo Escano, Sandra Spencer Miko, Yerin Kim, Joseph A. Waldron, Kim Do, Kyle MacPherson, Katie A. Yuen, Thilelli Taibi, Marty Yue, Aaremish Arsalan, Zhen Jin, Glenn Edin, Aly Karsan, Gregg B. Morin, Florian Kuchenbauer, Fabiana Perna, Martin Bushell, Ly P. Vu   +23 more
doaj   +2 more sources

Translational Capacity of a Cell Is Determined during Transcription Elongation via the Ccr4-Not Complex [PDF]

Cell Reports, 2016
The current understanding of gene expression considers transcription and translation to be independent processes. Challenging this notion, we found that translation efficiency is determined during transcription elongation through the imprinting of mRNAs ...
Ishaan Gupta, Zoltan Villanyi, Sari Kassem, Christopher Hughes, Olesya O. Panasenko, Lars M. Steinmetz, Martine A. Collart   +6 more
doaj   +2 more sources

The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.

PLoS Genetics, 2012
To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell.
Yoshie Tange, Atsushi Kurabayashi, Bunshiro Goto, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Jacqueline Hayles, Yuji Chikashige, Chihiro Tsutumi, Yasushi Hiraoka, Fumiaki Yamao, Paul Nurse, Osami Niwa   +12 more
doaj   +3 more sources

The CCR4-NOT complex maintains liver homeostasis through mRNA deadenylation. [PDF]

Life Sci Alliance, 2020
The biological significance of deadenylation in global gene expression is not fully understood. Here, we show that the CCR4–NOT deadenylase complex maintains expression of mRNAs, such as those encoding transcription factors, cell cycle regulators, DNA damage response–related proteins, and metabolic enzymes, at appropriate levels in the liver.
Takahashi A, Suzuki T, Soeda S, Takaoka S, Kobori S, Yamaguchi T, Mohamed HMA, Yanagiya A, Abe T, Shigeta M, Furuta Y, Kuba K, Yamamoto T.   +12 more
europepmc   +4 more sources

The CCR4-NOT complex suppresses untimely translational activation of maternal mRNAs.

Development, 2023
ABSTRACT Control of mRNA poly(A) tails is essential for regulation of mRNA metabolism, specifically translation efficiency and mRNA stability. Gene expression in maturing oocytes relies largely on post-transcriptional regulation, as genes are transcriptionally silent during oocyte maturation.
Soeda S, Oyama M, Kozuka-Hata H, Yamamoto T.   +3 more
europepmc   +3 more sources

The Ccr4-Not complex monitors the translating ribosome for codon optimality. [PDF]

Science, 2020
Coupling translation and mRNA decay Gene expression requires messenger RNAs (mRNAs)—DNA-derived blueprints of genes—to be translated by protein-producing ribosomes. The levels of mRNAs are tightly regulated, in part by controlling their half-lives.
Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R.   +16 more
europepmc   +4 more sources

Human Ccr4–Not complexes contain variable deadenylase subunits [PDF]

Biochemical Journal, 2009
The Ccr4–Not complex is evolutionarily conserved and important for regulation of mRNA synthesis and decay. The composition of the yeast complex has been well described. Orthologues of the yeast Ccr4–Not components have been identified in human cells including multiple subunits with mRNA deadenylase activity.
Lau, N.C., Kolkman, A., van Schaik, F.M.A., Mulder, K.W., Pijnappel, W.W.M., Heck, A.J.R., Timmers, H.T.M.   +6 more
openaire   +3 more sources

The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome. [PDF]

PLoS ONE, 2009
BACKGROUND:Ccr4-Not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including Not5 and the major yeast deadenylase Ccr4.
Nowel Azzouz, Olesya O Panasenko, Geoffroy Colau, Martine A Collart   +3 more
doaj   +1 more source