The Ccr4-Not Complex Regulates Skn7 through Srb10 Kinase [PDF]
Eukaryotic Cell, 2007 ABSTRACT The Ccr4-Not complex is a multifunctional regulatory platform composed of nine subunits that controls diverse cellular events including mRNA degradation, protein ubiquitination, and transcription. In this study, we identified the yeast Saccharomyces cerevisiae osmotic and oxidative stress ...
Lenssen, Eve +4 more
openaire +6 more sources
The CCR4 and CAF1 Proteins of the CCR4-NOT Complex Are Physically and Functionally Separated from NOT2, NOT4, and NOT5 [PDF]
Molecular and Cellular Biology, 1999 The CCR4-NOT complex (1 mDa in size), consisting of the proteins CCR4, CAF1, and NOT1 to NOT5, regulates gene expression both positively and negatively and is distinct from other large transcriptional complexes in Saccharomyces cerevisiae such as SNF/SWI, TFIID, SAGA, and RNA polymerase II holoenzyme.
Bai, Yongli +5 more
openaire +4 more sources
The multifunctional Ccr4–Not complex directly promotes transcription elongation [PDF]
Genes & Development, 2011 The Ccr4–Not complex has been implicated in the control of multiple steps of mRNA metabolism; however, its functions in transcription remain ambiguous. The discovery that Ccr4/Pop2 is the major cytoplasmic mRNA deadenylase and the detection of Not proteins within mRNA processing bodies have raised questions about the roles of the Ccr4–Not complex in ...
Jennifer A, Kruk +4 more
openaire +3 more sources
Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization [PDF]
Genome Biology, 2021 Background Regulation of protein output at the level of translation allows for a rapid adaptation to dynamic changes to the cell’s requirements. This precise control of gene expression is achieved by complex and interlinked biochemical processes that ...
Sarah L. Gillen +5 more
doaj +2 more sources
RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
Scientific Reports, 2022 Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization.
Aude Guénolé +7 more
doaj +2 more sources
Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation [PDF]
RNA, 2010 The CCR4-NOT complex is the main enzyme catalyzing the deadenylation of mRNA. We have investigated the composition of this complex in Drosophila melanogaster by immunoprecipitation with a monoclonal antibody directed against NOT1.
Temme, C. +7 more
openaire +3 more sources
The Not5 subunit of the ccr4-not complex connects transcription and translation. [PDF]
PLoS Genetics, 2014 Recent studies have suggested that a sub-complex of RNA polymerase II composed of Rpb4 and Rpb7 couples the nuclear and cytoplasmic stages of gene expression by associating with newly made mRNAs in the nucleus, and contributing to their translation and ...
Zoltan Villanyi +8 more
doaj +9 more sources
Architecture of the ubiquitylation module of the yeast Ccr4-Not complex. [PDF]
Structure, 2015 The Ccr4-Not complex regulates eukaryotic gene expression at multiple levels, including mRNA turnover, translational repression, and transcription. We have studied the ubiquitylation module of the yeast Ccr4-Not complex and addressed how E3 ligase binds cognate E2 and how it is tethered to the complex.
Bhaskar V, Basquin J, Conti E.
europepmc +6 more sources
The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.
PLoS Genetics, 2012 To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell.
Yoshie Tange +12 more
doaj +3 more sources
Reconstitution of Targeted Deadenylation by the Ccr4-Not Complex and the YTH Domain Protein Mmi1
Cell Reports, 2016 SummaryCcr4-Not is a conserved protein complex that shortens the 3′ poly(A) tails of eukaryotic mRNAs to regulate transcript stability and translation into proteins. RNA-binding proteins are thought to facilitate recruitment of Ccr4-Not to certain mRNAs,
James A W Stowell +2 more
exaly +3 more sources