Results 171 to 180 of about 2,118,837 (302)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Cell-Free DNA-Derived Immune Cell Ratios Uncover Cancer-Associated Systemic Changes. [PDF]

Cancer Res Commun
Andersen L, Lindskrog SV, Nordentoft I, Frydendahl A, Nors J, Henriksen TV, Rasmussen MH, Iversen LH, Gotschalck KA, Jensen JB, Dyrskjøt L, Andersen CL, Birkbak NJ.   +12 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

cfGWAS reveal genetic basis of cell-free DNA end motifs. [PDF]

Nat Commun
Zhu H, Zhang Y, Li L, Zeng S, Zhang X, Lin Y, Ou R, Wang L, Li X, Wang Y, Zeng J, Lin Y, Xu C, Zeng G, Li L, Zhao R, Jia Y, Luo F, Yang M, Hu Y, Xiao H, Xu X, Wang J, Zhou A, Zhang H, Jin X.   +25 more
europepmc   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

DNAvi: integration, statistics, and visualization of cell-free DNA fragment traces. [PDF]

Bioinformatics
Hess A, Seelow D, Kretzmer H.
europepmc   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcoma. [PDF]

EMBO Mol Med
Richardson SA, Safrastyan A, Karimpour M, Gulati G, Harker PJB, Redfern A, Pearce SP, Makeev VJ, Brennan B, Lee ATJ, Clipson A, Hill SM, Dive C, Rothwell DG, McCabe MG, Mouliere F.   +15 more
europepmc   +1 more source

Circulating Cell-Free DNA

, 2019
Yingli, Sun,, Ke, An,, Caiyun, Yang,
openaire   +3 more sources

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source