Whole genome deep sequencing analysis of cell-free DNA in samples with low tumour content
BMC Cancer, 2022 Background Circulating cell-free DNA (cfDNA) in the plasma of cancer patients contains cell-free tumour DNA (ctDNA) derived from tumour cells and it has been widely recognized as a non-invasive source of tumour DNA for diagnosis and prognosis of cancer ...
Devika Ganesamoorthy +9 more
doaj +1 more source
Endogenous cell-free DNA in fetal bovine serum introduces artifacts to in vitro cell-free DNA models
BioTechniques, 2022 Cell-free DNA (cfDNA) is of growing clinical and research significance. In vitro cfDNA models are a useful tool in cfDNA research; however, artifacts in these models may have implications for the interpretation of new and published data.
Bonnita Werner +2 more
doaj +1 more source
Circulating cell-free DNA levels correlate with postresuscitation survival rates in out-of-hospital cardiac arrest patients [PDF]
, 2014 Early prediction of prognosis is helpful in cardiac arrest patients. Plasma cell-free DNA, which increases rapidly after cell death, is a novel biomarker for the prognosis of critical ill patients.
Huang, Chien-Hua;Tsai, Min-Shan;Hsu, Chiung-Yuan;Chen, Huei-Wen;Wang, Tzung-Dau;Chang, Wei-Tien;Ma, Matthew Huei-Ming;Chien, Kuo-Liong;Chen, Shyr-Chyr;Chen, Wen-Jone +1 more
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Balkan Medical Journal, 2020 Background: Cell-free DNA, which may be considered as “liquid” biopsy, may serve as a diagnostic and prognostic marker not only in hematological malignancies but in solid tumors as well.
Zeynep Arzu Yegin +6 more
doaj +1 more source
Nature Communications, 2018 Methylation patterns of circulating cell-free DNA (cfDNA) contain rich information about recent cell death events in the body. Here, we present an approach for unbiased determination of the tissue origins of cfDNA, using a reference methylation atlas of ...
J. Moss +22 more
semanticscholar +1 more source
Noninvasive prenatal diagnosis of fetal RhD status using cell-free fetal DNA in maternal plasma with TaqMan® real-time PCR assay [PDF]
, 2007 Prenatal diagnosis is now part of established obstetric practice in many countries. However, conventional methods of prenatal diagnosis of obtaining fetal tissues for genetic analysis, including amniocentesis and chorionic villus sampling, are invasive
Rekhviashvili, Tea
core +1 more source
Study the Role of Cell Free DNA in the Diagnosis of Hepatocellular Carcinoma, an Egyptian Study
Asian Pacific Journal of Cancer Biology, 2022 Background and objective: Hepatocellular carcinoma (HCC) is a primary malignancy of the liver. Since the conventional tissue biopsy and AFP have limited value, a new promising diagnostic method” liquid biopsy” has emerged.
Noha Hassan Radwan +3 more
doaj +1 more source
CELL-FREE DNA TO DETECT HEART ALLOGRAFT ACUTE REJECTION
Circulation, 2021 Background: After heart transplantation, Endomyocardial biopsy (EMBx) is used to monitor for acute rejection (AR). Unfortunately, EMBx is invasive and its conventional histologic interpretation has limitations.
S. Agbor-Enoh +21 more
semanticscholar +1 more source
Cell-free DNA in the circulation as a non-invasive biomarker for breast cancer [PDF]
, 2013 Breast cancer remains the most common cancer among women worldwide. Although standard screening methods such as clinical breast examination (CBE) and Full-Field Digital Mammography (FFDM) are non-invasive itself, their sensitivity and specificity is ...
Kohler, Corina
core +1 more source
Cell-free DNA comprises an in vivo nucleosome footprint that informs its tissues-of-origin
Cell, 2016 Summary Nucleosome positioning varies between cell types. By deep sequencing cell-free DNA (cfDNA) isolated from circulating blood plasma, we generated maps of genome-wide in vivo nucleosome occupancy, and found that short cfDNA fragments harbor ...
M. Snyder +4 more
semanticscholar +1 more source