Results 41 to 50 of about 3,251,205 (352)

Cell‐free DNA as a biomarker of aging [PDF]

Aging Cell, 2018
AbstractCell‐free DNA (cfDNA) is present in the circulating plasma and other body fluids and is known to originate mainly from apoptotic cells. Here, we provide the first in vivo evidence of global and local chromatin changes in human aging by analyzing cfDNA from the blood of individuals of different age groups.
Teo YV, Capri M, Morsiani C, Pizza G, Faria AMC, Franceschi C, Neretti N.   +6 more
openaire   +3 more sources

Circulating biomarkers to monitor cancer progression and treatment

Computational and Structural Biotechnology Journal, 2016
Tumor heterogeneity is a major challenge and the root cause of resistance to treatment. Still, the standard diagnostic approach relies on the analysis of a single tumor sample from a local or metastatic site that is obtained at a given time point. Due to
Suthee Rapisuwon, Eveline E. Vietsch, Anton Wellstein   +2 more
doaj   +1 more source

Analysis of Plasma Cell-Free DNA by Ultradeep Sequencing in Patients With Stages I to III Colorectal Cancer

JAMA Oncology, 2019
This cohort study investigates the use of longitudinal data from ultradeep sequencing of cell-free DNA in Danish patients with colorectal cancer.
T. Reinert, T. Henriksen, E. Christensen, Shruti Sharma, R. Salari, H. Sethi, M. Knudsen, I. Nordentoft, Hsin-Ta Wu, Antony S. Tin, Mads Heilskov Rasmussen, Søren Vang, S. Shchegrova, Amanda Frydendahl Boll Johansen, R. Srinivasan, Z. Assaf, M. Balcioğlu, A. Olson, S. Dashner, D. Hafez, S. Navarro, Shruti Goel, M. Rabinowitz, P. Billings, S. Sigurjonsson, L. Dyrskjøt, R. Swenerton, A. Aleshin, S. Laurberg, A. Husted Madsen, A. Kannerup, K. Stribolt, Søren Palmelund Krag, L. Iversen, K. Gotschalck Sunesen, C. J. Lin, B. Zimmermann, C. Lindbjerg Andersen   +37 more
semanticscholar   +1 more source

DNA methylation analysis explores the molecular basis of plasma cell-free DNA fragmentation

Nature Communications, 2023
Plasma cell-free DNA (cfDNA) are small molecules generated through a non-random fragmentation procedure. Despite commendable translational values in cancer liquid biopsy, however, the biology of cfDNA, especially the principles of cfDNA fragmentation ...
Yunyun An, Xin Zhao, Ziteng Zhang, Zhaohua Xia, Mengqi Yang, Li Ma, Yu Zhao, Gang Xu, S. Du, Xiangan Wu, Shuowen Zhang, Xin Hong, Xin Jin, Kun Sun   +13 more
semanticscholar   +1 more source

Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]

, 2013
Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Himabindu Gali, Mórocz Mónika, Downes C. Stephen, Raskó István, Haracska Lajos, Downes, C. Stephen, Gali Himabindu, C Stephen Downes, Mórocz, Mónika, Lajos Haracska, Mónika Mórocz, Gali, Himabindu, Raskó, István, Haracska, Lajos, István Raskó   +14 more
core   +1 more source

Multimodal analysis of cell-free DNA whole-genome sequencing for pediatric cancers with low mutational burden

Nature Communications, 2021
Sequencing of cell-free DNA in the blood of cancer patients (liquid biopsy) provides attractive opportunities for early diagnosis, assessment of treatment response, and minimally invasive disease monitoring. To unlock liquid biopsy analysis for pediatric
Peter Peneder, A. Stütz, D. Surdez, M. Krumbholz, S. Semper, Mathieu Chicard, Nathan C. Sheffield, G. Pierron, E. Lapouble, Marcus Tötzl, B. Ergüner, D. Barreca, André F. Rendeiro, A. Agaimy, H. Boztug, G. Engstler, M. Dworzak, M. Bernkopf, S. Taschner-Mandl, I. Ambros, O. Myklebost, P. Marec-Berard, S. Burchill, B. Brennan, S. Strauss, J. Whelan, G. Schleiermacher, C. Schaefer, U. Dirksen, C. Hutter, K. Boye, P. Ambros, O. Delattre, M. Metzler, C. Bock, E. Tomazou   +35 more
semanticscholar   +1 more source

Biochemical and clinical diagnostic aspects of circulating nucleic acids [PDF]

, 2005
We investigated the size distribution of fetal DNA using a combination of agorose gel electrophoresis, Southern blot and real time PCR assay. Our results showed plasma DNA presents apoptotic characteristics and the cell free fetal DNA is generally ...
Li, Ying
core   +1 more source

In silico Design of Linear DNA for Robust Cell-Free Gene Expression

Frontiers in Bioengineering and Biotechnology, 2021
Cell-free gene expression systems with linear DNA expression templates (LDETs) have been widely applied in artificial cells, biochips, and high-throughput screening.
Xinjie Chen, Yuan Lu
doaj   +1 more source

High-intensity sequencing reveals the sources of plasma circulating cell-free DNA variants

Nature Medicine, 2019
Accurate identification of tumor-derived somatic variants in plasma circulating cell-free DNA (cfDNA) requires understanding of the various biological compartments contributing to the cfDNA pool.
P. Razavi, Bob T. Li, David N. Brown, B. Jung, E. Hubbell, R. Shen, W. Abida, K. Juluru, Ino de Bruijn, C. Hou, O. Venn, R. Lim, A. Anand, T. Maddala, S. Gnerre, Ravi Vijaya Satya, Qinwen Liu, Ling Shen, N. Eattock, J. Yue, A. Blocker, M. Lee, A. Sehnert, Hui Xu, M. Hall, Angie Santiago-Zayas, W. Novotny, J. Isbell, V. Rusch, G. Plitas, A. Heerdt, M. Ladanyi, D. Hyman, David R. Jones, M. Morrow, Gregory Riely, H. Scher, C. Rudin, M. Robson, L. Diaz, D. Solit, A. Aravanis, J. Reis-Filho   +42 more
semanticscholar   +1 more source

Limitations and opportunities of technologies for the analysis of cell-free DNA in cancer diagnostics

Nature Biomedical Engineering, 2022
Cell-free DNA (cfDNA) in the circulating blood plasma of patients with cancer contains tumour-derived DNA sequences that can serve as biomarkers for guiding therapy, for the monitoring of drug resistance, and for the early detection of cancers.
P. Song, L. Wu, Yan Helen Yan, J. Zhang, T. Chu, Larry Kwong, Abhijit A. Patel, D. Zhang   +7 more
semanticscholar   +1 more source