Results 41 to 50 of about 12,684,378 (242)

Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]

, 2013
Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Himabindu Gali, Mórocz Mónika, Downes C. Stephen, Raskó István, Haracska Lajos, Downes, C. Stephen, Gali Himabindu, C Stephen Downes, Mórocz, Mónika, Lajos Haracska, Mónika Mórocz, Gali, Himabindu, Raskó, István, Haracska, Lajos, István Raskó   +14 more
core   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Sequence analysis of cell-free DNA derived from cultured human bone osteosarcoma (143B) cells

Tumor Biology, 2018
The true importance of cell-free DNA in human biology, together with the potential scale of its clinical utility, is tarnished by a lack of understanding of its composition and origin.
Abel Jacobus Bronkhorst, Johannes F Wentzel, Vida Ungerer, Dimetrie L Peters, Janine Aucamp, Etienne Pierre de Villiers, Stefan Holdenrieder, Piet J Pretorius   +7 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Adenylylation of Gyrase and Topo IV by FicT Toxins Disrupts Bacterial DNA Topology [PDF]

, 2015
Toxin-antitoxin (TA) modules are ubiquitous molecular switches controlling bacterial growth via the release of toxins that inhibit cell proliferation.
Gerdes, K., Glatter, Timo, Dehio, Christoph, Alexander Harms, Goepfert, A., Scheu, P., Kenn Gerdes, Glatter, T. ; https://orcid.org/, Schirmer, T., Harms, Alexander, Christoph Dehio, Harms, A., Timo Glatter, Stanger, Frédéric Valentin, Gerdes, Kenn, Dehio, C., Schirmer, Tilman, Harms, Alexander; id_orcid, de Jong, I., de Jong, Imke Greet, Stanger, F., Tilman Schirmer, Patrick Daniel Scheu, Arnaud Goepfert, Scheu, Patrick Daniel, Frédéric Valentin Stanger, Imke Greet de Jong, Goepfert, Arnaud, Harms A, de Jong IG, Gerdes K, Stanger FV, Dehio C, Glatter T, Schirmer T, Scheu PD, Goepfert A   +36 more
core   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.

PLoS Neglected Tropical Diseases, 2020
BACKGROUND:Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp.
Jingkai Ji, Bin Li, Jingzhong Li, Wangmu Danzeng, Jiandong Li, Yanping Zhao, Gezhen Qiangba, Qingda Zhang, Nibu Renzhen, Zhuoga Basang, Changlin Jia, Quzhen Gongsang, Jinmin Ma, Yicong Wang, Fang Chen, Hongcheng Zhou, Huasang, Jiefang Yin, Jiandan Xie, Na Pei, Huimin Cai, Huayan Jiang, Huanming Yang, Jian Wang, Asan, Xiumin Han, Junhua Li, Weijun Chen, Dong Yang   +28 more
doaj   +1 more source